Manzanillo, Sheena Lhane B.

BSN2H

M3 - Exercise 3. Pre-lab

1. Click this link http://learn.chm.msu.edu/vibl/content/antimicrobial.html# (Links to an

external site.) and do the activity in Antimicrobial Susceptibility.
2. Determine and compare the sensitivity of the 3 bacteria, E. coli, P. aeruginosa, and S.
aureus, to the different antibiotics used, as to whether: Resistant, Intermediate, or
Susceptible.
3. In the measurement of the Zone of Inhibition, follow the procedure in this video,
Microbiology resources - How to Measure Zones of Inhibition (ZOI)
E. coli

After completing the activity, I discovered that E. coli isolates were highly resistant to
amoxicillin, erythromycin, clindamycin, and other antibiotics. Meanwhile, the only intermediate to
E is tetracycline. coli. Finally, cephalothin, chloramphenicol, ciprofloxacin, streptomycin,
tobramycin, and trimethoprim sulfa are all effective against it.
P. aeruginosa

Meanwhile, in P. aeruginosa, I've noticed that most antibiotics are resistant, with only
chloramphenicol being intermediate and ciprofloxacin and tobramycin being susceptible.
 S. aureus

I've noticed that the majority of the bacteria isolated with S. aureus are resistant, and the
only susceptible antibiotic is vancomycin.

So, I conclude that S. aureus strains are penicillin-resistant but can still be sensitive to
penicillin such as oxacillin and methicillin, and E. coli bacteria can develop resistance to beta-
lactam antibiotics by producing extended spectrum beta-lactamase (ESBL). Last but not least, P.
aeruginosa is intrinsically resistant to a variety of antimicrobial treatments due to its
impermeability.