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Biochemistry 215 Lab report 6-7

Molar calculations, stock preparation and dilutions
pH measurement and buffer preparation
10 marks
1. Determining the pH and the identity of three unknowns (1.5)

Unknown pH Value Obtained Acidic, Basic or Neutral? Give an example of a solution

solution or anything you know with a
similar pH
A
B
C

2. Answer the following questions

a. You are given 1 L of 1M stock solution. Do the calculation for 100 mL of the following
concentrations (1.5)

Final Concentration Stock Solution (ml) Water or Buffer (ml)

needed (M)
0.8

0.5

0.25
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For the table above: Show your work below for ONE CALCULATION ONLY and include units.
b. DESCRIBE STEPWISE How would you prepare 100 mL of 0.25 M KNO3 solution? (Molar
mass of KNO3= 101.1032 g/mol) (2)

c. A chemist dissolves 98.4 g of FeSO4 in enough water to make 2.000 L of solution. What
is the molarity of the solution? (Molar mass of FeSO4 = 151.9076 g/mol) (2)

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d. Battery acid is generally 3M H2SO4. Roughly how many grams of H2SO4 are in 400 mL of
this solution? (Molar mass of H2SO4 = 98.07848 g/mol) (2)

e. Which of the following can act like a buffer and why? (1)
CH 3 COOH
1. A solution containing equimolar quantities of acetic acid and sodium acetate
2. A solution containing equimolar quantities of sulphuric acid and sodium
sulphate

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