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KAPIL
KAPIL
Introduction:
The organ bath is a traditional experimental set-up that is commonly
used to investigate the physiology and pharmacology of in vitro tissue
preparations. Perfused tissues can be maintained for several hours in a
temperature controlled organ bath.
Some of the tissues that may be studied with an organ bath system
include:
Smooth Muscle:
Guinea-pig ileum
Uterine
Colon
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STUDENT ORGAN BATH
Skeletal Muscle:
Mammalian Diaphragm
Cardiac Muscle:
Atrium
Ventricle
Schemat
ic diagram of a custom-made mounting system for tissue rings.
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Panlab Organ Baths:
A heating rod is built into each of the organ bath units and a thermostat
controller is included to control the water temperature surrounding the
tissue chambers (Figure 2). Pre-heating reservoir coils are also
positioned within the water bath to heat the perfusate before entering the
tissue chamber. A highly efficient, magnetic water pump is built into the
water bath to circulate the heated water and ensure even heat
distribution throughout the entire system. Manipulators/micropositioners
are supplied for accurate positioning of the transducers and applying
pre-tension to the tissue preparations prior to experimentation.
Figure 2.
Key Benefits
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This unit incorporates a high wattage heater for rapid warming of the
water bath and a two-stage force and suction pump that provides water
circulation. Circulation of the water is necessary to maintain a constant
temperature in the reservoir and tissue chamber jackets.
Specifications
2L water-jacketed reservoir
Transducer Positioners
Oxygenating Bubblers
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STUDENT ORGAN BATH
Support Stand & Hardware Kit
Force Transducers (Isometric)
Cable & Connector Package
High-Tech Tissue Organ Baths (5-300 ml; User must specify size
when ordering)
Bridge Amplifiers
Thermo Bath/Heater
PowerLab Data Acquisition System
LabChart Pro (including Dose Response)
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The above product codes are for combination universal support
rods/stimulating electrodes; however, the electrodes may be purchased
separately to be interchanged on a universal support rod.
Key Benefits
Large range: of tissue/organ baths volumes (5,10, 25, 50, 100, 200, 300
ml)
Transducers:
Isometric Transducers
Isotonic Transducers
Stimulation and Electrode Selection
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Field stimulation may be performed using ring or plate electrodes that
are placed near (but do not touch) the tissue and have a current passed
between them. This method is effective provided that the tissue remains
within the field of stimulation and is the most common technique used in
organ bath preparations.
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strength by observing
kymograph paper.
15. Release the clamp of organ
bath to let the PSS flow
outside the organ tube if you
want to reduce the strength of
dose, if you want to increase
the dose, no need to
change the PSS. (It is for same
drug)
16. For every new drug
injection the old PSS must be
change.
17. Ensure the temperature
of water bath; it should not
increase than optimum
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temperature.
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strength by observing
kymograph paper.
15. Release the clamp of organ
bath to let the PSS flow
outside the organ tube if you
want to reduce the strength of
dose, if you want to increase
the dose, no need to
change the PSS. (It is for same
drug)
16. For every new drug
injection the old PSS must be
change.
17. Ensure the temperature
of water bath; it should not
increase than optimum
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temperature.
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STUDENT ORGAN BATH
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6. Thermostat
7. Stirrer or mixer
8. Oxygen tube
9. Aerator
10. Leaver
11. Load
12. Sherrington rotating drum
(Kymograph Drum)
13. Kymograph Paper
Use- When we have to
perform the invitro studies of
any given drug, we need to
isolate
the organ from suitable
experimental animal. Once
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5. Heater
6. Thermostat
7. Stirrer or mixer
8. Oxygen tube
9. Aerator
10. Leaver
11. Load
12. Sherrington rotating drum
(Kymograph Drum)
13. Kymograph Paper
Use- When we have to
perform the invitro studies of
any given drug, we need to
isolate
the organ from suitable
experimental animal. Once
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Gas flow to the buffer is normally controlled with a two-stage regulator
designed for the gas mixture utilized. Flow rates of 0.5-2 liters per minute
and/or pressures of 1-2 psi are adequate for most preparations. The
aeration stream can be controlled with a needle valve and should be a
steady line of fine bubbles that do not cause a pronounced “boiling”
effect in the bath. Higher pressures will only serve to increase
evaporation, jostle the preparation and create noise in the force trace
and may perforate glass frits used for aeration. For thin tissues with a
large surface area and low metabolic activity (such as blood vessel strips
or rings), aeration using room air may be sufficient; in these cases
simple aquarium pumps are a cost effective replacement for compressed
air tanks.
Advantages:
The tissue must be isolated from the body, which can alter
physiological responses.
The tissue can only be studied for a limited time before it
deteriorates.
The results obtained may not be directly applicable to in vivo
physiology.
Materials:
Safety:
Always wear personal protective equipment (PPE) when handling
the isolated organs.
Avoid contact with the bath solution to prevent skin irritation.
Do not exceed the recommended temperature range for the organ
of interest to prevent damage to the tissue.
Follow proper procedures for disposal of biological materials.
Data Analysis and Interpretation:
Analyze the collected data according to the specific protocol and
report the results.
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Compare the results to the expected outcomes and interpret any
discrepancies.
References:
Provide any relevant references or protocols used in the
development of the SOP.
Include any guidelines or recommendations from the manufacturer
of the organ bath instrument.
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Experiment (1)
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After the experiment, carefully remove the heart from the bath and dispose of
the used solution according to established protocols.
Result:
The results of the experiment should show an increase in the heart rate and
contractility in response to increasing concentrations of epinephrine.
The experiment should also show that epinephrine causes an increase in the
strength and frequency of cardiac contractions, as predicted by the theory.
The result can be analyzed using graphs and statistical analysis.
Experiment (2)
Aim: To investigate the effect of different temperature on the contractile
activity of isolated frog skeletal muscle.
Requirement:
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Procedure:
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The result can be analyzed using graphs and statistical analysis.
Conclusion:
The student organ bath is a useful tool for studying the physiology
of isolated organs. It allows researchers to conduct experiments on
isolated organs in a controlled environment, which can provide
valuable insights into the physiological mechanisms of the organ in
question. The SOP for a student organ bath provides a clear and
detailed guide on how to set up and conduct an experiment, as
well as how to analyze and interpret the data collected.
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