Name: Raevon Roopchan Class: 4 West Teacher: Miss Mohammed-Khan

Line:4L4 Subject: Biology (2021-2023) Date Given: 03/02/2022

Page number: Lab # 4

Skills Assessed: Observation, Recording, Reporting(ORR), Analysis and Interpretation(AI)

Title: Enzymes

Aim: To identify the effect pH and temperature have on enzyme activity

Apparatus and Materials:

 A small box of matches

 Masking tape
 A block of ice in a cooler
 Timer

Procedure:

1. Obtain a small box of matches, masking tape, a block of ice and a timer
2. Record in 10 second intervals, noting how many matches placed. Do this for a total of 60
seconds. That will be “Control”
3. After that, wrap hand with masking tape. Again, see how many matches can be placed in 60
seconds, using 10 second intervals. That will be “pH”
4. For the final part, place hand in cooler containing block of ice for one minute. See how many
matches can be placed every 10 seconds. Record for a total of 60 seconds. This will be
“temperature”
5. Record number of matches placed in each and place in table
6. Make a graph where seconds will be x axis and control/pH/temperature will be y axis

Drawing of experimental setup:

Results: The “control” has the greatest number of matches laid. The “pH” should have less than the
control. The “temperature” should have the least

Trends: When temperature and pH decrease, the enzyme activity will decrease as a result.

Discussion: