Professional Documents
Culture Documents
Research Notes
Research Notes
*Select a study
-Narrow down your research problem and questions the best you can.
*Narrow down your search
Tips:
1. Focus only on the terms found in your title
E.g.
Autibacterial Activity of Hexatoxic Extract of Cynthilium cenereum on E.coli.
2. Make an outline of information you are going to gather
3. Create a list of possible search terms you are going to write in the search engine
*Search and choose literature
Tips:
1. Start looking for general references
2. Look for at least 5 primary sources on a certain topic
-to look on internet, type “Scholarly articles on… *type search term here*”
I- Introduction
M- Materials and methods
R- result
A- and
D- Discussion
*Try your best to look for recent studies(5 years or younger)
IV. Bibliography
V. Appendices
A. Duration and schedule of activities
B. Proposed budget plan
C. Forms
D. Data gathering instrument(for applied)
D. preliminary tests( for life)
Introduction
Introduction is the part where you are going to answer the questions:
- why did I conduct this study?
- why is it worth studying?
- what did we know about it before I did this study?
Orient the reader with the rationale behind the work, with the intention of defending it. It places your
work in the theoretical context, and enables the reader to understand and appreciate your objectives.
Types of SOTP
- General SOTP
-Specific SOTP -->Descriptive, Inferential
Example:
Termiticidal Property of Citrus maxima peeling extract on Coptotermes formunatus
General SOTP
This study aims to determine the termiticidal property of Citrus maxima peeling extract on
Coptotermes formunatus
Specific SOTP
Specifically, this study aims to answer the ff. questions:
Descriptive
What is the termiticidal property of formunatus in terms of mortality rate (in %) 24 hours after
application of treatments?
Inferential
Does the Citrus maxima peeling extract have a significant termiticidal property at Coptotermes
formunatus in terms of mortality rate (in %) 24 hours after application of treatments?
1. What is the tremiticidal property of the different concentrations (100%, 50%,& 25%) of C. maxima
extract of C. formunatus in terms of mortality rate (in %) 24 hours after application of treatments?
2. Is there a significant difference on the tremiticidal property of the different concentrations (100%,50%,&
25%) of C. maxima peeling extract on C. formunatus in terms of mortality rate (in %) 24 hours after
application of treatments?
3. Which concentration (100%,50%,& 25%) of C. maxima peeling extract has the same sig. Highest
termiticidal property on C. formunatus in terms of mortality rate (in %) 24 hours after application of
treatments?
Engineering:
Example:
Ocean Wave Generator
SOTP:
Generally, this study aims to design & create an ocean powered generator
Specifically, this study aims to answer the ff. Questions
1. What is the capability of OWG in producing electricity in terms of a)power rating (in W),b)voltage
output(in V) & time (in hrs) to fully charge a 3.7 V Li-ion battery
2. What is the acceptability of OWG in terms of a) operationability, b) ease of use, c) uniqueness ass
rated by 10 engineers
Methodology
Purpose:
This portion describes in sufficient detail the procedures employed in the research so that it can be
evaluated and repeated (if necessary)
Answers the question:”how did you solve the problem?”
Valid- the procedure and the data gathered are relevant and appropriate to the problem investigated
Reliable- the data gathered can be reproduced
2. Control
Types of control:
Negative control(-)
A control group or treatments which is expected to be not effective.
Positive control(+)
A control group or treatments which is expected to be effective. another term that’s used is standard
treatment
3. Replication
-Number of times a setup was conducted
-replications aims to increase the accuracy of treatment comparison, and give the experimenter valid
estimate of the magnitude of the experimental error
-The minimum number of replications per treatment is three, but the more the number of replications,
the better.
4. Randomization
Randomization is a precaution against unforseen disturbance or for damage control, if unforseen
disturbances occur. In every stage, randomization must be produced unless bias will be introduced.
-can be introduced when assigning the treatments aand replication to the setups
-there are varied randomiazation techniques, but the most common one is the use of lottery method
5. Experimantal layout design
Method of assigning the treatments and replicates to test organism, materials, or setup
B. Randomized Complete Block Design- A desgn to use when the test organism or media is not entirely
homogeneous
Example of RCBD
I II III
A B A
B A B
Legend:
A- Quassia indica Leaf extract
B- Water (negative control)
Experimental Layout
1. How many treatments & replications?<-----first sentence
2. What experimental design will you use?<-----second sentence Passive Voice
3. How will you assign treatments to your test organism/setup?
Experimental Layout
_______________________________________________________________________________________
_______________________________________________________________________________________
____________________________________________________________________________________
Applied------------------------------------------------------------------------------------------------------------------------------------
Design and specification:
This portion describes what are the part of the device and its characteristics or specifications.
In describing each part and the device has plenty of parts, it’s better to group the parts based on its
purposes.
The first paragraph just generally state its parts. Proceeding paragraphs would describe each part in
detail.
----------------------------------------------------------------------------------------------------------------------------------------------
Life-----------------------------------------------------------------------------------------------------------------------------------------
DATA COLLECTION PROTOCOL
Experimental proper:
This refer to the assays or the description of how experimental procedures are carried out.
If you had a basis In the assay you are going to use, better state first the name of the assay(if it is a
standard one or has a name) or which related study did you based you procedure[example: Based on the
procedure used in the study of Tantiado (2014)]
If you are going to not fully follw the suggestions of the standard procedure mention it(“e.g. adapted
from that of WHO(2015)protocol” or “based on the WHO (2015) protocol with some modifications”)
Just to make sure that you are going to detail those modifications.
Describe the procedures for your study in sufficient detail that other scientists could repeat your work
to verify your findings. Do not forget: Arrange your assay chronologically.
There will be some data that all you need is to observe, count, or measure. There will be also data that
still need computations. In this case, indicate what formula you are going to use.
Data Analysis
You will indicate how the data will be summarized and analyzed. This will also include standards that
you may use(that do NOT employ inferential statistical procedure).
This includes
1. Qualitative analysis ir scale(with citatons)
2. Statistical software used
3. How the data were summarized(means, percent, etc.)
4. How you are reporting measures or variability(SD, SEM, 95% confidence levels, etc.)
5. Which data transformations were used (to correct normal distributions or equalize variances)
6. Inferential statistical tests used with reference to the particular questions or kinds of questions they
address
7. Any other numerical(e.g. normalizing data) or graphical techniques used to analyze data.
8. What probability (a prori) was used to decide significance; usually reported as the Greek Symbol alpha
(α)
This may include sex, age, typical size(height, weight, length) whether they are fresh or dried. For
genetic studies, the strain or genetic stocks maybe used.
Example:
Cyanthillium cinerium leaves. One kilogram of fresh, both young and mature leaves of Cyanthillium
cinerium will be used in the study. It wll be ensured than the leaves to be used are free from insect bites,
discoloration and molds which are signs of insect and microbial infestation
Escerichia coli. K12 strain of E. coli which was cultured at West Visayas State University will be used in
the study.
Gathering of materials
Indicate when, where, and how the organisms will be gathered
Tips:
In purhcasing bacteria or organisms, indicate the source (e.g. Philippine National Culture of
Microorganisms or PNCM Los Baños, Laguna or the name of the Laboratory Shops)
In gathering plant materials, it was advised to gather the plant prior to sunset
Extraction procedure
Indicates the detailed procedure employed in extracting substances
On the first sentence, indicate already the name of the method of extraction used in the study
if the method to be used has no known name but used former researchers, just type “… were based
according to the procedures employed bu author last name (year)”
Use standard procedures. If you are going to use other methods of extraction, you must have a valid
reason of doing so
Look for the internet for step by step procedures in doing extraction procedures
----------------------------------------------------------------------------------------------------------------------------------------------
Ethanolic
Methanolic
Aqueous
Hexanoic
----------------------------------------------------------------------------------------------------------------------------------------------
Hypothesis
*alternative hypothesis[research hypothesis(H a)]
*null hypothesis (Ho)
SOTP
1. What is the moluscicidal activity of the diff. conc.(20mg/mL, 40mg/mL, 60mg/mL, 80mg/mL,) of M.
charantia leaf exract on pomacea canaliculata in terms of mortality rate (%) 20 hrs. After application of
treatments?
2. Is there a sig. Diff. On the moluscicidal activity of the diff. conc.(20mg/mL, 40mg/mL, 60mg/mL,
80mg/mL,) of M. charantia leaf exract on pomacea canaliculata in terms of mortality rate (%) 20 hrs. After
application of treatments?
3. Which among the diff. Concentrations (20mg/mL, 40mg/mL, 60mg/mL, 80mg/mL,) M. charantia leaf
exract is the most effective as an molluscicidal on pomacea canaliculata in the terms of mortality rate (%)
24 hrs. After application of treatments?
Ha
1. 1.The diff. conc. (20mg/mL, 40mg/mL, 60mg/mL, 80mg/mL,) of M. charantia leaf exract has molluscicidal
activity on P. canaliculata in terms of mortality rate(%) 24 hours after application of treatments.
----------------------------------------------------------------------------------------------------------------------------------------------
Ho
1. The diff. conc. (20mg/mL, 40mg/mL, 60mg/mL, 80mg/mL,) of M. charantia leaf exract has no
molluscicidal activity on P. canaliculata in terms of mortality rate(%) 24 hours after application of
treatments.
----------------------------------------------------------------------------------------------------------------------------------------------
Disposal procedure
Indicate what will happen to the setups, materials and organism you used after the experiment has
been conducted.
Preliminary tests
This will refer to the tests you will have to conduct first prior to main testing to help you determin
whether you should proceed to main testing, helps you formulate research design (e.g. treatments) or to
dry run your main experiment to forsee possible difficulties in conducting the experiment
Examples of preliminary tests
Phytochemical screening. Phytochemical screening is also a simple test used to indicate a plant extract’s
pharmaceutical and insecticidal potential.
Preliminary tests
Put it in Appendix and place it in laboratory manual form (directive, step by step form)
Research plan
First part:
1. TITLE- bold
2. Researchers- bold, last name first
A. Question of problem being adressed (bold)
Intro(bold)
B. Goals/expected outcomes/hypothesis(bold)
SOTP & hypothesis
Null inferential hypothesis
GRADE 10---------------------------------------------------------
Statistical Guide and Interpretation
(What Statistical Tool to Use)
What is Statistics?
Statistics is a discipline of study dealing with the collection, analysis, interpretation and presentation of
data(Stephens, 1998)
Branches of Statistics
Descriptive Statistics- concerned with the gathering classification & presentation of data and
summarizing of values to describe group characteristics of data. Its purpose is to reduce information
into manageable size and put it into focus.
Inferential Statistics- is concerned with the analysis of subset of data leading to predictions of
inferences about the entire set of data.
Variable vs Constant