SpecGuardTM GCPH

 Rapid sterilization
 High efficiency and broad spectrum
 Mild and non-irritant

Spec-Chem
2020.3.20
 Contents

1 Development and Background of SpecGuardTM GCPH

2 Information of SpecGuardTM GCPH

3 Rapid Sterilization of SpecGuardTM GCPH

4 Application of SpecGuardTM GCPH

 1 Development and Background

Microbial threats from nature: Pyococcus and Bacillus, Enteric

pathogen, Skin pathogen, Fungi and Mycotoxin, Viruses, etc.

The outer structure of bacteria: the cell wall components include

peptidoglycan, phospholipic acid, lipoprotein, and
lipopolysac7309ride, etc. The cell membrane is an elastic semi-
permeable membrane composed of phospholipid bilayer and
immobilized protein.
 1 Development and Background

Coronavirus (an enveloped virus)

Structure of viruses: some viruses have an outer envelope, which
is mainly derived from host cells, but also embedded with
proteins, mainly composed of proteins, lipids and
polysaccharides. The inner layer is nucleocapsid, which is
composed of shell pellet units and arranged regularly. The main
components are capsid protein and genetic material.

Envelope viruses: HIV, EB virus, Herpes zoster virus, etc.

Envelopeless viruses: Poliovirus, Coxsackie virus, Echo virus, etc.
 1 Development and Background

Bacterial cell walls and viral envelope have very similar composition, high concentration of
alcohols can directly inactivate bacteria and viruses, and a low concentration of alcohols will
also destroy the spatial structure of proteins and their derivatives to a certain extent, these
include specific protein derivatives on the surface of the virus, which can not kill the virus
directly, but effectively destroy the way of virus invasion.

 SpecKare® 7309 has a very strong ability to chelate metal ions, it can block the way for microorganisms to obtain metal
ions. At the same time, the lipophilicity of the alkane chain helps to break through the microbial membrane，and
compared with other acid products，SpecKare® 7309 is suitable for the pH range of 5.0~6.5, consistent with the pH of
the skin。
 As a natural plant extract, the fruit extract of CITRUS PARADISI, rich in flavonoids, not only has anti-inflammatory and
antioxidant effects, but also destroys the integrity of plasma membrane by inhibiting the activity of microbial enzymes,
After the action of grapefruit fruit extract, the cell membrane was
killing a variety of bacteria and viruses, thus playing a role of multiple regulation and synergism in the formula. It is safe damaged and the cytoplasm flowed out
and non-toxic to human body. Environmentally friendly and biodegradable. Good compatibility, can be compatible with
other natural medicine, and also has synergistic effect.
 As a kind of auxiliary sterilizing agent , diol solvent is very helpful to reduce the surface tension of the system and even
the microbial cell membrane, so as to facilitate the penetration of the microbial membrane.
1 Development and Background

Viral infection pathways:

• The virus needs receptors to enter the host cell to complete
its replication
• The coronavirus receptor is angiotensin-converting enzyme
Ⅱ
• The human body's mucosal cells (mouth, nose, etc.) are
distributed with the coronavirus receptor - angiotensin-
converting enzyme Ⅱ

Commercially available products:

• Alcohol and chlorinated products in contact with mucosal
cells can cause intense irritation and even tingling.
Meanwhile, the ethanol concentration is often less than 75%
in lots of Gel Sanitizer, dose it really work?
• Severe cases include allergic reactions
• Not suitable for daily skin contact and sterilization
• Surfactants and antioxidants claim to be antibacterial and
have weak bacteriostatic properties
• Single component, not broad spectrum, easy to develop drug
resistance

Customer needs: a very gentle, suitable for daily skin care,

efficient, broad spectrum, compound collaboration, a safe
sterilization product !
 2 Information of SpecGuardTM GCPH

Product Name： SpecGuardTM GCPH Specification

INCI Name： 1,2-HEXANEDIOL，PENTYLENE GLYCOL，CITRUS
Items Specification
PARADISI (GRAPEFRUIT) FRUIT EXTRACT, SpecKare®
7309 ，ETHYLHEXYLGLYCERIN Appearance Pale yellow transparent liquid
Usage： Add in the last step, both high and low temperature ,but avoid
Odor 7309racteristic
prolonged high temperature.
Dosage： 1.2-5.0%. Refractive Index（20℃） 1.350-1.550
Package: Customized.
Storage： Keep in dark and cool place. Specific Gravity（20℃） 0.900-1.050

Shelf life： 2 years.

pH（10%，20℃） 2.0-4.5
Application： Water formulations, various emulsifying systems.
Preservative Free： YES
 2 Information of SpecGuardTM GCPH

SpecKare® 7309

Ionization rates of organic acid at different pH values

 It's made up of two parts: octyl and hydroxamic acid. It has the
best length of antibacterial carbon chain (C8) and a strong pH value 3 4 5 6 7
chelating group. SpecKare® 7309(7309) 100 100 100 100 100
 The length of this chain effectively penetrates cell membranes,
altering permeability. On the other hand, hydroxamic acid has Dehydroacetic Acid 99.5 94.9 65.1 15.7 1.8
good chelation with Fe2+ and Fe3+, preventing the growth of mold. Benzoic Acid 94.1 61.3 13.7 1.6 0.2
 In addition, ocoxiamic acid is an extremely weak organic acid and
can act in neutral to alkaline environments. This characteristic Sorbic Acid 98.3 84.9 36 5.3 0.6
makes it stand out among many acid preservatives.
Salicylic Acid 48.3 8.5 0.9 0.1 0

Anisic Acid 96.9 76.0 24.0 3.1 0.3

Levulinic Acid 97.5 79.9 28.5 3.8 0.4

pKa of SpecKare® 7309=9.56±0.20，and even at pH 8, more than

70% of the 7309 is in the form of conjugate acid, acid preservatives
work only in this form. Compared with the extremely lower effective pH
range of other acids, 7309 has a wider range of pH applicability and is
more gentle and non-irritating to the skin.
The Structure of SpecKare® 7309(7309)
 2 Information of SpecGuardTM GCPH

SpecKare® 7309—Safety

No clinically significant
potential for dermal irritation
or allergic contact sensitization！

Safety Assessment of SpecKare® 7309 as

Used in Cosmetics-Cosmetic Ingredient
Review in USA-May 10 2019
 2 Information of SpecGuardTM GCPH

SpecKare® 7309—Minimal Inhibitory Concentration(MIC)

Minimal Inhibitory Concentration(MIC) Test of SpecKare® 7309

Method: Microtubule Broth Method.
Sample Treatment: 0.8w% propylene glycol solution (initial concentration), dilution gradient is twice.
Inoculation: Bacteria(105~106CFU); Fungus(104~105CFU).
Escherichia coli Staphylococcus aureus Pseudomonas aeruginosa Candida albicans Aspergillus niger Propionibacterium acnes
Microorganism (E. coli) (S. aureus) (P. aeruginosa) (C. albicans) (A. niger) ATCC16404 （P. acnes）
ATCC8739 ATCC6538 ATCC9027 ATCC10231 ATCC11827

ppm 310 1250 1250 160 80 310

MIC
w% 0.031 0.125 0.125 0.016 0.008 0.031

SpecKare® 7309 has a broad spectrum, particularly strong against mould.

SpecKare® 7309 has a strong inhibitory effect on P. acnes.
 2 Information of SpecGuardTM GCPH

Glycols： PrzvFree® HLG ，PrzvFree® PTG and PrzvFree® EHG

PrzvFree® HLG：1,2-Hexanediol is a very pure moisturizer, solvent. At the same

time, it has relatively good antibacterial ability, which is very mild and widely used
in the world.

PrzvFree® PTG：Pentylen glycol is a multi-functional ingredient, it can

Ethylhexylglycerin CAS No.:70445-33-9

moisturize the skin, reduce the irritation and allergy caused by preservatives. At IECSC--Inventory of Existing Chemical Substances in China
the same time, it has certain bacteriostatic ability. EINECS—European Inventory of Existing Commercial Chemical Substances
DSL- Canada Domestic Substances List

PrzvFree® EHG：Ethylhexylglycerin, it has a moisturizing effect, improve the

AICS—Australia Inventory of Chemical Substances

skin feeling. Przvfree® EHG can effectively inhibit the growth and reproduction of NZloC—New Zealand Inventory of Chemicals
odor-causing bacteria without affecting the beneficial skin flora. Particularly ECL—Korean Existing Chemicals List
important, Przvfree® EHG can reduce the surface tension of the microbial TCSI—Taiwan Chemical Substance Inventory
membrane, can play a synergizing and antibacterial role in the formulation. PICCS—Philippine Inventory of Chemicals and Chemical Substances
 3 Rapid Sterilization of SpecGuardTM GCPH

Test Sample：wipes solution+1.2% SpecGuardTM GCPH，10mL

Test method：according to the CTFA 7309llenge test method, the modified details include microbial inoculation amount and
detection method.

Test Strains: (Gram Negative）Escherichia coli ATCC 8739; Pseudomonas aeruginosa ATCC 9027; (Yeast) Candida albicans
ATCC 10231; (Mould) Aspergillus niger ATCC 16404; (Gram Positive) Staphylococcus aureus ATCC 6538;

Concentration of bacterial & fungi suspension and inoculation amount

Escherichia coli Staphylococcus Pseudomonas Candida albicans Aspergillus niger

Strains
aureus aeruginosa

Concentration of
1.7×109 3.2×109 1.0×109 2.4×106 2.0×107
bacterial suspension
(CFU/mL)

Inoculation
100 100 100 200 100
amount(μL)

Total bacterial inoculation：5.9×108CFU; Total fungi inoculation：2.5×106CFU

 3 Rapid Sterilization of SpecGuardTM GCPH

Culture: the bacteria were cultured on TSA plate at 36℃ for 24~48h.The fungi were cultured in Rose Bengal medium plate at
28 ℃ for 3 ~ 5 days.

Test Results

Positive control

Sterilize for 1 min Sterilize for 3 min

 3 Rapid Sterilization of SpecGuardTM GCPH

Test Results

Duration 1min 3min Positive control

Bacteria（CFU/dish） ND ND TNTC

Log Reduction
6.8 6.8 /

Fungi（CFU/dish） 8 ND TNTC

Log Reduction
4.4 4.4 /

ND: Not Detected; TNTC: Too Many To Count.

Conclusion：The results showed that the rapid bactericidal rate of the wipes solution (1.2% SpecGuardTM GCPH) after treatment for
1min and 3min were on the same level. The Log reduction values of 1min and 3min were approximately 6.8 (bacteria) and 4.4
(fungi). The bacteria can be completely killed in 1min, and the fungi can survive in a very small amount. Bacteria and fungi can be
completely killed in 3min.
 4 Application of SpecGuardTM GCPH

Reference formula: Mild facial disinfectant wipes

Product Name Supplier/INCI Name w/w% Function

Specchem/ 1,2-HEXANEDIOL， Moisturize,

PENTYLENE GLYCOL，CITRUS antibacterial,
A SpecGuardTM GCPH PARADISI (GRAPEFRUIT) SEED 1.2 antifungal,
EXTRACT，SpecKare® 7309, conditioner,
ETHYLHEXYLGLYCERIN antiviral
EDTA-2Na 0.05 Chelating agent
GLYCERIN 1.5 Moisturize
Specchem/ C8-C10ALKYL
B SpecSufc® APG0810 0.2 Cleaning
GLUCOSIDE
pH controlling
NaOH pH to 5.5
agent
WATER To 100
Process: Mix B phase evenly, add A phase, continue to stir until uniform and transparent, adjust pH
to 5.5.
 4 Application of SpecGuardTM GCPH

Compatibility:
SpecGuardTM GCPH is water-soluble, and is compatible with most cosmetic materials and has good solubility in water and emulsion
systems. It can be cold matched and not easy to separate out at low temperature.

Application:
SpecGuardTM GCPH can be used in a variety of systems: aqueous system, emulsion system, anhydrous formulation and surfactant
system.For example: hand sanitizer, cleaning lotion, wipes, deodorant spray, hand cream, etc.

Usage:
SpecGuardTM GCPH can be added at high and low temperature under normal cosmetic processing technology, without affecting its
bactericidal efficiency. However, it contains natural active ingredient, so it is necessary to avoid prolonged high temperature.

Dosage:
1.2~5.0%; Water-based formula、neutral and partial acid conditions, dosage can be reduced as appropriate. If rich in polymer, oil or
alkaline, dosage should be increased.