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ERGOFIT I4ICROIVIXAQUA

ERGOFITMICRO MIX AOUA

TECHNICALPRODUCTBULLETINI8.67
USEPA,OIL PROGMN,ICENTER
LISTINGDATE: JULY27,2011
"ERGOFIT14ICROMIX AQUA"

I. NAIYE,BMND, ORTMDEMARK
ERGOFIT
T4ICRO
MIX AQUA
Biorernediation
Asent(l,licrobiological
Culture,EnzynEAdditlve,Nutrient
IISfi,glito**'
II, NAME,ADDRESS,
ANDTELEPHONE
NUN1BER
OF IVANUFACTURER/CONTACT
ER@FITUSALLC
40 E. MainStreet#379
Newark,DE19711
Phoner(302)235-3085
Fax:(801)846-8043
f4obile:(98s) 722-8442or Gro) 739-9773
E-rEil i iEfojOtrlCIQEdX:UEangm
Web Slte| !yldtw,mhrc0d}:!se.!gn0
(lVr. WarrenRussell,US Factory Representative)

III. NAN4E,
ADDRESS,
ANDTELEPHONE OF PRIN4ARY
NUI4BER DISTRIBUTORS
Svsten6Research Intematlonal,
Inc.
13 HendtageCourt
Destrehan,LA 70047-3626
Phone:(985)764-1692
(985)722-8882
14obile:
WebSite: wl$u,Eisletri)c]Jsa,llE
(!lr. RamRanbchandran, PrincipalEngineer)

IV. SPECIAL
HANDLING
ANDWOR|<ER
PRECAUTIONS
FORSTORAGE
ANDFIELDAPPUCATION
1. FlamnEbilltvi Water-based, Non-flamnEble
2. VentilationiNeedsno ventilation;aqueous-based organicproduct;doesnot emit
hazardousvapors. conE co2 rEy buildup.
3. Skinand eye contact; protective clothing;treatrnent in case of contact: ER@FIT|4ICRO
MIX AQUAis not a definalirritant. Avoid eye contact, and wear goggleswhen usingproduct.
Eyewashfor quickand copiouseye flushingshouldbe available on site. Seekpronpt rnedical
attentionshouldcontactoccurand if irritationDe6ists.Protectiverubberolovesare
suggestedduringhandling.ERGOFIT MICROl'.4liAQUAhas a srnellof fenne-ntationand yeast,
The product does not give off harrful vapors. Longstoragewill cause buildCO2in water
vapor
4.a. lYaxiftumstoragetetrperuture: 104oF
4,b. l..4ininum
storagetenperaturei 32oF
4.c. Optinumstoragetemperature rangei57oFto 97oF
4.d. Ternperatures of phaseseparation and chenicalchanges:above113oF

V. SHELFLIFE
ERmFIT MICROl4IX AQUAhas a recomnEndedshelf life of 5 years. After 5 years at
optimumstorage in cool temperaturethere is an approxifiEte10Vodecreaseper year In
producteffectiveness.Shelterfrom direct sunlightor extrenE cold or heat source.In the
event of fire, wash with water.

VI. RECOM14ENDED
APPLICATIONPROCEDURE
1. AppllcationMethod: ERGOFIT I4ICROMIX AQUAcan be appliedwith any conrnercially
availablesprayapparatus,suchas srnallhandheldtanks,backpacks,largemixingtanks
pumping
with rnechanical devices,vesselswith boornsfor sprayingwide paths,or liquidspray
deviceson croDduster aimlanesor helicoDters-ERCOFIT I!4ICRO
tlIX AOUAcan also be
appliedby eduator systenb from vessels,fire trucks, or any other outfiited vehicle.

2, Concentmtion/Application Rate: General. ER@FITMICROIYIXAQUAgenerallytakes 5 to

50 minutesto penetratethe nDlecularwalls of hydrocarbons.Wannerconditionsfavor faster
biologicalactionwhereascolderconditions fiEy reducethe speedof deconposition. once
ERGOFIT N4ICRO MIX AQUAis appliedto the targeted areas,the nlcrobes willattach
thenEelves to the hydrocarbonsand start to attack their carbonstructureand rEtabolize
into naturalorganicsubstancesfrom the hunus fanily. Aquatic plants and anlnEl life will
feed on this nutrient,Withinhoursodorwill be negligentand changeswillbe noticeable in
seven days, dependingon the tenperature. ERGOFIT MICROIYIXAQUAwill also breakdown
the adhesivepropertles of the hydrocaftons, preventingoilsfromattachingto aninEls,
vegetation,rock5,and earth.

Dilution Rat€sr ERGOFIT IYICRO IYIXAOUAshouldalwavs be nixed wlth the natlve water of
the affectedarea.Note: Do not use clty waterthat has beentreatedwith chlorinewith
ERGOFIT IYICROl4IX AQUA,This meansthat if you are treatjng an affected saltwater area,
then saltwatershouldbe usedas your basecanlerfor the product.Ukewiseif you are
treating a lake or river, then that carrierwater would be the freshwaterfrom that source.
For generalapplicationsand light oil filr6, dilute each gallonof ERGOFIT MICRO|v X AQUA
with sourcewater (e.q.,fresh,brackish, or saltwater)at a ratioof 1:50,Foroil rpusse like
fonrbtionln Intertldalzones,usea dllutionof 1r30.If the splllls very heavyand the oll layer
is thick (rDre than 1 inch) it is reconrEnded that ERGOFIT l4ICRoMtX AQUAbe appliedat a
lower ratio of 1r20, On land not Involvlngwater bodies,it Is recorffnendedthat an organlc
absorbentbe appliedpriorto usingERGOFIT MICRON4IXAQUAto treat oil spillsto renEdiate
the C1 and C35Petroleum Hydrocarbons, Thls ls to increasethe contactsurfaceareafor
ERGOFIT IYICROlvllx AQUAto conveit spillsoil and absorbentto organichufius.

Appllcationon CoastalWat€r: Applythe n xed solutlonusihghandheldsprayers,tank

sprayers,boo116 from vessels,helicopters,or ainplahes.First, staft by spraylngthe outer
pedheter of the spilland systenEticallywork towards the middleof the affected area,
nEkingsure to cover the entire surface area of the spill. Use 1.5 gallonsof ERGOFIT IYICRO
N1IXAQUAconcentratefor every 1000 gallonsof hydrocarbonsspilled.Dlute it with 30
gallonsof seawdterper gallonof concentratefor sprdying.[To calculategallohsof ERGOFIT
I4ICROllIX AQUArequired,nultiply estimatedgallonsof hydrocarbonsby 0.0015.1For
denserhydrocarbons,like in the case of crude oil, multiplybarrelsof crude oil by 42 to get
gallohsof crude and apply above ratios. lf you do not know gallonsof hydrocarbonsor
barrelsof crude oil, estirnatesize of spillby (1000 squareyards x 2 inch averagethickness)
to arrive at gallonsof oil spilled.Use 10 kg/22lbs of ER@FITI4ICRON4IXAQUAdilutedto 50
gallonsin water to cover this area. As a rule, 2 kgl4.4 lbs ERGOFIT MICROl4IX AQUAis
requiredper netric ton of oil spilled.Dilutionvariesdepending on oilthicknessor sinple
sheenfloatingon water surface.

Application for Intertidal zones: EROFIT 14ICRO 14IXAQUAshouldbe appliedat low tide,
when the affected areas are exposedto the elenEnts,allowingthe productto securely
attach to the hydrocarbons, Ceaseapplication if the tide risesand resumeoncethe water
levelhas recededagain.Continueapplication cycleuntilthe entireaffectedareahas been
coated,The recomrEnded mixratiois 1:30.In areaswherea combination of hightide and
waveshavepushedthe contamination beyondthe intertidalzone, oil rd9 layerlookinglike
chocolaterbusse. cordonoff the area and start spravinqERGOFIT MICROl.4IXAOUAdiluted
at a ratioof 1:20 in water.The objectiveis to get an application rdte of betwee;1 Lo2
gallonsof concentratedERGOFIT |CRO MIX AQUAsprayedper 1000gallonsof hydrocarbons
soilled,
3. Conditionsfor Use: ERGOFIT lvlICROllIX AQUAacts on all organichydrocarbonson sea,
fresh water, and land. As the aqe of spilledhydrocartonsincreases,so doesthe tirne
necessaryfor bioremediation. In general,fresh crude, gasolineof BTEXtakes anywherefrom
30 days to 12 nbnths to conpletely biorernediate dependingon weather conditionsand
density of spill. However,the rer€diation processwill be noticeablethroughoutthe process.
Variations in seawatersalinity,has no effect, as longas microbial life can exist,then
ERGOFIIN4ICRO l..4IX
AQUAwill be effective in salt and brackishwater with a rnaximum
salinityof 4.9 percent. ER@FITt4ICROMIX AQUAbiorernediation slows sornewharar
temperaturesbelow40oF.However,it will continueto work in any water temperaturcsthat
will sustalnmicrobialIife. Decreasingthe arnountof ER@FITMTCRO |VIXAouA per square
yard from recomnEndeddosagerdtes lengthensthe renEdiatlontjrne. When nitrogenand
carbon is addedto ERGOFIT 14ICRO MIX AQUAthe inltiationof bacterial-enzynEticactivity is
enhancednEhy fold to beginbioren€diation within2 hoursof application.

VII. TOXICITYANDEFFECTIVENESS
a. Toxicity:
NA
b, Effectiveness:
BiorenEdiationAgent EffectlvenessTest (40 CFR300.900),FederaiRegisterSeptember15,
19941
SunrrEry DataTablel
DAYS PRODUCT TOTALMEAN REDO/O TOTALItlEAN REDO/O
3 REPS/PROD ALKANES (ppm) (ppm)
28 DAYS AROIVATICS 28 DAYS
. CoNTROL 239,235 0 16,502 0
" PRODUCT 239,686 0 17,431 0

, CoNTROL 217,023 9.30 L0,942 33.70

' PRODUCT 17t819 92,6 9,606 44,90

10 CoNTROL 188,379 21.3 8,989 45.50

PRODUCT 2,216 99.10 3,100 82.20
Resultsof GravirnetricAnalysis:
Percentage(o/o)Decreasein Weight of Oil on Day 28
C ontr ol :1.7 %
Product:45,60/0

V[I. 14ICROBIOLOGICAL
ANALYSIS
Microbiological Culturei
by speclesand percentagein the conposition: CONFIDENTIAL
1. Listingof all microorganisnE
2. OptimumpH, tenperature, and sElinityrdngesfor use of the additive:
pH: 6.5 to 7,5.
I er Def t t t ure : 5 /' F to 9 /-F .
SalinityrThe maxirrumpermissible salinityis up to 4.9olosodiumbV solution
3, Mininilmand rnafmumpH, tenperature, and salinitylevelsbelowor above which the
effectivenessof the additiveis reducedto half its optimumcapacityi
pH: Lowerthan 4.5 or higherthan 8,5 reducesthe productefficiencyby 50 percent.
Tenperature: Lowerthan 32oFor higherthan 104"F reducesthe product efficiencyby 50
percent.
Salinity:The nExinxlmpermissible salinity is up to 4,9olosodiumby solution
4. Specialnutrjent reeuirernents:NA
5. Test resultsrcgardingthe determinationof the presenceof the following:
Salrr|)nella:Neoative
Fecalcoliform:Neqative
Shigella:Negative
Staphylococcus Coagulasepositive:Negative
Beta HernclyticStreptococci:Negative

EnzyrneAdditive:

1. Ustingof each conponent of the total fonrulation, other than enzyrnes,by chemical
narE and percentageby weight: CONFIDENTIAL
2. EnzynENan€si CONFIDENTIAL
3. I.U.B.:CONFIDENTIAL
4. Sourceof Enzymes:Flant or Fungi/Fennentation process
5. unitsr No lessthan 1oloahd no nbre than 50o/obv weiqht
6. SoecificGravitvi1.00
7. OptlrrumCohditions:
a. p H: 7,0
b. Temp€rature:72oF
c. SalinityRanges:Freshwater to salt water up to 4.9Vo
d. I\4inimumand lvaxinumoHi 4.5 - 8.5
e. I\4inimumand l\4axlnumiemperaturer 32oF- 104oF
f. lYlnimumand l4axlrrumSallnltyLevelsi Sallnltylevelthat will support nicrobia
actlvltv wlll not adversely
actlvlty adverselva ERGOFIT N,IICRO
IYICROMIX AQUA gerfonnance
AOUAperfonnance
Enzynt Shelf Life: ljp to 5 years when properlystored
EnzynEOptlnElStordgeConditlons:72oFls optinEl, enzyrp rangeis freezing
to 104oF,never leave ERGOFIT MICRO|v X AQUAin dlrect sunllghtfor rnore
than a coupleof hours

NutrientAdditivei
1. Listingof each cofiponent of the formulatlonby cherlcal nan€ and percentageby
weight: CONFIDENTIAL
2. Optlrrumstorageconditionsi
Temperaturei32oFto 104oF

PHYSICAL
PROPERTIES
NA

X. ANALYSIS
OF HEAVY14ETALS.
CYANIDE,
ANDCHLORINATED
HYDROCARBONS