Score:

/50
GENERAL BIOLOGY 1
Laboratory Worksheet 1: The Compound Light Microscope
2nd Period, A.Y. 2021-2022

Name: Lance Matthew N. Pedrajas Section: T-STEM 12 E2 Professor:

Mr. Harold Ray Landingin Date Performed: January 19, 2023

A. Label the parts of the microscope. (18 points)

Ocular
Lens/Eyepiece

Revolving Head
Nosepiece
Arm/Handle
Objective/Objective Lens
Mechanical Stage Safety Rack Stop
Course Focus/Course
Clip/s Adjustment
White - HPO
Blue - OIO
Mechanical Stage
Red - Scanning Fine Focus/Course
Condenser
Yellow - LPO
Base
Adjustment

Iris Diaphragm
Light Source Light Switch

https:// www.walmart.com/
ip/AmScope-40X- 1600X-LED-
Binocular- Compound-
Microscope-New/ 39549420

B. Using the microscope: 3 points

each for the drawing (3x3=9)

Criteria 3 points 2 points 1 point

Color
The color of the drawing reflects that of the
image seen.
Completeness
The student satisfies all the The student satisfies 2 of The student satisfies only 1 of
The drawing shows the entire image as seen
three criteria listed the criteria listed the criteria listed
under the microscope.
Accuracy
The drawing is detailed with all parts
presented.

Figure 1. The parts of a compound light microscope

Laboratory Worksheet 1: The Compound Microscope Page| 1

Senior High School Department
1st Trimester/ A.Y. 2019-2020
 Letter “e” before viewing

Sketch of the letter e using the LPO. Sketch of the letter e using the HPO

GUIDE QUESTIONS: Be concise in answering the following questions.

Rubric for the 1st three questions and Q5:

2 points- if the response is correct
0 point- if the response is incorrect

1. What happens to the position of letter e when viewed under the microscope? Is it the same as when viewed
with the naked eye or is it inverted? (2 points)

The position of the letter e tends to become inverted and upside down whenever it is viewed under the
microscope.

2. When you move the slide to the left, in which direction does the letter image appear to move? (2 points)

The letter e moves to the opposite direction whenever the slide is moved. In this case, e switches to the right if
the slide is situated in the left.

3. When you move the slide towards you, in which direction does the letter image appear to move? (2 points)

The direction of the letter e tends to move upwards whenever the slide is moved towards the observer which is
downwards.

4. Explain your observations on the three questions above. (3 points)

Rubric
3 points-Explanation is clear with correct supporting details.
2 points- Explanation is somewhat clear with few correct supporting details.
1 point- Explanation is vague with no supporting details.

Whenever the slide is moved towards a certain direction in the microscope’s stage, the test subject or in this case, the
letter e, shifts to the direction opposite to what has been done in the slide. This results to the inverse proportionality
between the direction of the slide’s movement and the direction in where the test subject moves toward.

5. When you shift to HPO, what happens to the letter image? How about the field of view? ( 2 points)

The letter image: The view of the letter image tends to be larger and therefore, making it easier and more detailed to
examine and analyze.
Laboratory Worksheet 1: The Compound Microscope Page| 2
Senior High School Department
2nd Period/ A.Y. 2021-2022
Field of view: The image’s field of view decreases, making it smaller than the Scanning and LPO lenses counterparts.

6. What is the total magnification of your microscope when the given objective is in place?
(1point each= 1x4= 4 points). Do not forget to put “x” beside the total magnification value. No x=0.5 point.

Objective in Place Total Magnification

Scanner 40x
Low Power Objective (LPO) 100x
High Power Objective (HPO) 400x
Oil Immersion Objective (OIO) 1000x

C. Magnification/Reduction = Size of the drawing/size of the object. (8 points total)

Specimen Actual Size Computation for the Is it magnified or
Magnification/Reduction of a 5cm reduced?
drawing (1 point each)
(1 point each)
Hydrilla tooth cell 0.01 mm 10 mm
(diameter)
5 cm× = 50 cm Magnified
1 cm

50 cm
= 5000x
0.01 mm

Length of a potted 0.2 mm 10 mm

plant
5 cm× = 50 cm
1 cm
(Measure from the Magnified
part above the soil to
the tip)
50 cm
= 250x
0.2 mm

Rice grain (length) 25.3 mm 10 mm

5 cm× = 50 cm
1 cm
Reduced
50 cm
= 1.98x
25.3 mm

Laboratory Worksheet 1: The Compound Microscope Page| 3

Senior High School Department
2nd Period/ A.Y. 2021-2022