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    Iso 6887 1 1 20

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    Iso 6887 1 1 20

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    iso-6887-1-1-20

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    Iso 6887 1 1 20

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    INTERNATIONAL ISO STANDARD 6887-1 Second edition 2017-03 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions Microbiologie de la chaine alimentaire — Préparation des échantillons, de la suspension mére et des dilutions décimales en vue de examen microbiologique — Partie 1: Regles générales pour la préparation de la suspension mere et des dilutions décimales leference number 6887-1:2017(E) ‘ny has been made by information Center for Standards, Metsology and Quality © 1802017 2017(E) LN COPYRIGHT PROTECTED DOCUMENT © 1502017, Published in Switzeriand All rights reserved. Unless otherwise specified, no part ofthis publication may be reproduced or uilized otherwise in any form fr by any means, electronic or mechanical, including Photocopying. of posting on the internet ov an intranet, without prior ‘writen permission. Permission can be requested frm ether ISO atthe address below or ISO's member body in the country of the requester. 150 copyright office Gh. de Blandonnet @« cP 401 CH-1214 Vernier, Geneva, Switzerand Tel +4122 74901 11 Fax +41 22 74909 47 copyright@iso.org wwwis0.0rg i (© 150.2017 - Al rights reserved Contents Foreword... nn Introduction. - a 1 aeen 10 SCOPE one non co - Normative references... ne ‘Terms and definitions. co Principle - Diluents. - co 5.1 Basic materials Se 5.2 Diluents for general use. - 5.2.1 Peptone salt solution... - 5.2.2 Buffered peptone water. - 5.23 Double-strength buffered peptone water 53 Diluents for special purposes. 5.4 Distribution and sterilization of the diluent. 5.5 Performance testing for diluents. Apparatus, een Sampling Preparation of samples o-oo - 81 General... oe 82 Erozen products . Con 8.2.1 General cn 822 Small samples defrosted before testing 8.2.3 Large pleces or blocks sampled while frozen... 83° Hard and aty products... 8.4 Dehydrated and other low-moisture products 8.5 Liquid and non-viscous products ..--nunvoewennnn 86 Acidic products... 87 High-fat (over 20%) foods. Cn 88 — Multi-component products... sn 89 Packaged product... vo Surface samples (swabs and other devices) Specific procedures. 9.1 Test portion and initial suspension (primary dilution). 9.2. Duration of the procedure 93 Pooling and compositing procedures for qualitative tests, Further dilutions: - 10 Decimal dilution series... 10.2 Other dilution series ' Se ISO 6887-1:20171 (E) Page Annex A (informative) Illustrations of pooling and compositing procedures Annex B (informative) Method for sampling frozen test pieces or blocks. Annex C (informative) Data showing reliability of test results according to size of test portions. Annex D (informative) Verification protocol for pooling semples for qualitative tests. Bibliography... (© 150 2017 ~All rights reserved COC SREEYYVIAA DW UeeeRUUEY BR HHS 150 6887-1:2017(E) Foreword 150 (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through 1SO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International ‘organizations, governmental and non-governmental, it liaison with 1S0, also take part in the work, 150 collaborates closely with the International Electrotechnical Commission (EC) on all matters of electrotechnical standardization The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criterla needed for the

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