The document discusses properties of DNA polymerases including that they add nucleotides in the 5' to 3' direction, require a template and primer, are extremely accurate with proofreading activity, and replicate DNA quickly due to being processive. It also mentions telomeres which protect the ends of chromosomes, the end replication problem, and that telomerase is a ribonucleoprotein containing an RNA component and reverse transcriptase protein that functions as an RNA-dependent DNA polymerase to extend telomeres and regulate replication in eukaryotes.
The document discusses properties of DNA polymerases including that they add nucleotides in the 5' to 3' direction, require a template and primer, are extremely accurate with proofreading activity, and replicate DNA quickly due to being processive. It also mentions telomeres which protect the ends of chromosomes, the end replication problem, and that telomerase is a ribonucleoprotein containing an RNA component and reverse transcriptase protein that functions as an RNA-dependent DNA polymerase to extend telomeres and regulate replication in eukaryotes.
The document discusses properties of DNA polymerases including that they add nucleotides in the 5' to 3' direction, require a template and primer, are extremely accurate with proofreading activity, and replicate DNA quickly due to being processive. It also mentions telomeres which protect the ends of chromosomes, the end replication problem, and that telomerase is a ribonucleoprotein containing an RNA component and reverse transcriptase protein that functions as an RNA-dependent DNA polymerase to extend telomeres and regulate replication in eukaryotes.
• Also has proof-reading activity to correct errors
• Very fast reaction due to processive nature
Replication at chromosome ends
• In humans the telomere sequence is
TTAGGG. This sequence is usually repeated several thousand times • Telomeric proteins protects the chromosomal ends from damage End replication problem Telomerase: A ribonucleoprotein
TERT: Telomerase reverse transcriptase
TER: Telomerase RNA Annu Rev. Biochem. 1996
Telomerase: A RNA dependent
DNA polymerase Extension of the 5’ end of the chromosome Regulation of replication Bacteria Eukaryotes Helicase loading and activation Fundamentals of Cell Cycle Regulation Regulated helicase loading and activation