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Blood Grouping
Blood Grouping
BLOOD GROUPS
Hafizul Seikh
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Describe Rh incompatibility.
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SYNOPSIS
Introduction
The ABO System
Inheritance
Landstiners law
Agglutination (Clumping)
Crossmatching
HDN
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P Ii system
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ABO SYSTEM:
ANTIGEN: Any foreign invading substance which can evoke an
immune response in the body. Antigens of blood groups are
macromolecules, which occur on the surfaces of the red
blood cells and on different body cells called:
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Blood group Agglutinogen Agglutinin
A A AntiB
B B AntiA
AB A,B ---
O --- AntiA, AntiB
Q: If the Ag A is present on the Red cell, why is Anti A not
produced? Why is Anti B being formed in the plasma?
A: Ag A is present at birth and is recognized as self antigen hence
antibodies Anti A are not produced in response to antigen A entering
the body. However Anti B is produced in the plasma in response to
various antigens entering plasma via food, inhalation, bacteria etc.
Titer of Agglutinins at different ages:
At birth, concentration is zero starts at 2 to 8 months,
maximum at 8 to 10yrs, declines throughouthttps://www.nursingsathi.com
life.
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WHAT ARE BLOOD TYPES?
Blood Types
There are 3 alleles or genes for blood
type: A, B, & O. Since we have 2 genes, AA or AO = Type A
BB or BO = Type B
there are 6 possible combinations. OO = Type O
AB = Type AB
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Genotype OO A 1A 1 / A 2 A 2 / A 1B A2B BB /
A 1A 2 / A 2 O OB
A 1O
Phenotype O A1 A2 A1 B A2B B
/blood group
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IgG
IgM
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ANTIGENS & AGGLUTINOGENS OF BLOOD GROUPS:
Antigens of blood groups are macromolecules, occur on
the surfaces of red blood cells & on different body cells
Structures of surface – polysaccharides, glycoproteins,
lipoproteins
These particles of cells can be found in plants, in
vertebrates, in viruses and in bacteria, too
These antigens can be determined from the third
month of fetal life until the end of our lives
Production of antigens are controlled by the genes
inherited from both of the parents
At least 15 commonly occurring antigens are able
to cause blood transfusion reactions
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ANTIGENS OF O-A-B SYSTEM: 16
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ANTIGENS OF THE ABO SYSTEM ON THE
SURFACE OF RED BLOOD CELLS:
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H antigen A antigen B antigen20
G G
F F G F G
G
G G G
G G
G
G G G
C C C
G Galactose G N-acetylgalachttps://www.nursingsathi.com
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Nature of agglutinins:
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LANDSTEINER’S LAW:
States that
1) if an agglutinogen is present on the RBC
membrane, then the corresponding agglutinin must be
absent in the plasma.
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Compatibility between blood groups:
Major reaction-between Donor’s cells & recipient’s plasma
Minor reaction-Between Donor’s plasma & Recipient’s cells
CROSSMATCHING:
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Cross Matching (Major) 28
Group A
Group B
Group AB
Agglutination or
clumping of RBCs
Group O
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Possible complications of blood transfusions 29
1. Incompatibility reactions
Antigens on donor’s RBC + Antibodies in recipient’s plasma
RBC Agglutination
Clumps
I/V haemolysis release of Hb
may block
Haemoglobinaemia and Jaundice blood vessels
Renal failure
‘DEATH’
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2. Fever - due to the presence of antibodies against
transfused WBCs and platelets
3. Allergic reactions - asthma
4. Circulatory overload
5. Iron overload
6. Air embolism - air can go into the circulation
7. Transmission of diseases - Malaria, hepatitis B, AIDS,
Syphilis
* Rh Incompatibility
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FATE OF THE TRANSFUSION REACTIONS: 32