STEM Orosa PR2 Manuscript Group 8

Calatagan Senior High School
Practical Research 2
EFFECTIVENESS OF TOMATO AS HAND SANITIZER

FOR SOOTHING SKIN
A Research Presented to
CALATAGAN SENIOR HIGH SCHOOL
Poblacion 2, Calatagan, Batangas
In Partial Fulfillment
of the Requirements for
PRACTICAL RESEARCH 2
Science, Technology, Engineering and Mathematics
Eduardo L. Ilang Ilang

Ricardo A. Ilao Jr.
Althea A. Aytona
Joyce C. Bernal
Jhervie P. Morales
2022
Calatagan Senior High School ii
TABLE OF CONTENTS
Page
TITLE PAGE................................................................................................i
DEDICATION................................................................................................
ACKNOWLEDGEMENT...............................................................................
ABSTRACT..................................................................................................
TABLE OF CONTENTS...............................................................................
LIST OF TABLES.........................................................................................
LIST OF FIGURES.......................................................................................
CHAPTER
1. THE PROBLEM AND THE BACKGROUND
Background of the Study..............................................................................
Statement of the Problem.............................................................................
Significance of the Study..............................................................................
Scope, Limitation and Delimitation...............................................................
Hypothesis…………………………………………………………………….…
Definition of Terms……………………………………………………………...
2. REVIEW OF RELATED LITERATURE AND STUDIES
Research Literature......................................................................................
Related Studies............................................................................................
Synthesis......................................................................................................
Calatagan Senior High School iii
Conceptual Framework.................................................................................
3. METHODOLOGY
Research Design..........................................................................................
Research Environment.................................................................................
Respondents/Subject of the Study...............................................................
Data Gathering Procedure............................................................................
Statistical Treatment of Data........................................................................

Calatagan Senior High School 1
CHAPTER I
THE PROBLEM AND THE BACKGROUND
Background of the Study
Covid-19 is a virus that one can get not only through the contact
with an infected person, but also from the things that people touch in daily
activities without proper sanitation. According to World Health
Organization, from January 2020 to September 2022, there are almost 4
million confirmed cases in the Philippines, including the fatalities and
recoveries. Germs present on items that get onto hands can cause
different diseases and sickness, such as common cold and influenza. To
avoid the spread of germs, hand sanitizers containing at least 60% of ethyl
alcohol can be use (CDC, 2021). However, according to a study, habitual
use of this may result to dryness and irritation of skin. In agriculture,
tomato production had been a problem in the Philippines. Tomatoes were
thrown away by the farmers due to oversupply in some areas of our
country since the pandemic outbreak (GMA News Online, 2022).
Ripe tomato is a plant rich in Vitamin C, Lycopene, and beta-
Carotene. Vitamin C hydrates, brightens, and repairs damaged skin. In
order to reduce skin irritation, lycopene restores new skin cells. Beta-
Carotene is an antioxidant that keeps the appearance and healthiness of
the skin (Ebanel, 2020). Some well-known manufacturers of sunscreens,

hydration mask, skin cream, skincare booster, and soap use tomato as
one of their main ingredients because of its benefits on skin.
From the cited concern on existing hand sanitizers and benefits of
tomato, the researchers seek to develop an antibacterial and at the same
time skin soothing hand sanitizer using the plant and assess its
effectiveness.
Statement of the Problem
This study aims to develop an alternative soothing hand sanitizer
using a tomato plant that prevents skin irritation.
Specifically, this study seeks answers to the following questions:
1. What is the amount of:
1.1 Tomato Extract,
1.2 Ethyl Alcohol, and
1.3 Aloe Vera in different concentrations?
2. What is the outcome of Tomato Hand Sanitizer in terms of:
2.1 Acidity,
2.2 Soothing Effect,
2.3 Antibacterial Effect, and
2.4 Lifespan in different concentrations?
3. What is the appearance of the produced hand sanitizer in terms
of:
3.1 Smell,
3.2 Color, and
3.3 Texture in different concentrations?
4. Is there a significant difference in acidity level, soothing effect,
antibacterial effect, lifespan, smell, color, and texture of the produced
hand sanitizer among different concentrations?
5. What process can be done to enhance the effectiveness of
produced hand sanitizer?
Significance of the Study
The study entitled, “Effectiveness of Tomato as Hand Sanitizer for
Soothing Skin” will be significant to the following:
Environment. This study will be beneficial to the environment as it
can reduce the number of tomatoes being thrown due to its oversupply. In
addition, it can help the community because the hand sanitizer uses
affordable ingredients causing its price to be affordable as well, and will
help keep a healthy environment that is skin-friendly at the same time. It
can be used as a hand sanitizer instead of soap and water while traveling,
at malls, schools, especially in health care institutions.
Students. This research will be mainly beneficial to Science,
Technology, Engineering, and Mathematics (STEM) students for the
reason that the result of this will enhance their knowledge about the
benefits of tomato, more importantly its healing properties, and how they
can use it.
Future Researchers. The findings of this study will serve as a
guide for future researchers undertaking comparable study. Future
researchers will be driven by this study to innovate and acquire better
ideas about this topic in the future.
Hypothesis
Ho: There is no significant difference in acidity level, soothing
effect, antibacterial effect, lifespan, smell, color, and texture of the
produced hand sanitizer among different concentrations.
Ha: There is a significant difference in acidity level, soothing effect,
antibacterial effect, lifespan, smell, color, and texture of the produced
hand sanitizer among different concentrations.
Definition of Terms
For the purpose of clarification, the important terms used in this
study are defined conceptually and operationally.
Acidity. The level of acid in substances such as water, soil, or wine
(Oxford Languages). In this study, it is the level of acidity of the produced
Tomato Hand Sanitizer.

Aloe Vera. A gelatinous substance obtained from a kind of aloe,
used especially in cosmetics to soften or soothe the skin (Oxford
Languages). As used in this study, it is a substance of the produced hand
sanitizer that soothes skin.
Antibacterial. A substance that kills bacteria or stops them from
growing and causing disease (cancer.gov). As used in this study, it is one
of the characteristics of the produced hand sanitizer to be evaluated.
Antioxidant. A substance that may prevent or delay some types of
cell (National Center for Complementary and Integrative Health). In this
study, it specifically refers to a substance that protects skin cells in
Beta-Carotene. A plant pigment that is an isomer of carotene,
important in the diet as a precursor of Vitamin A (Oxford Languages).
Operationally, it helps maintain skin’s health and appearance present in
Disk Diffusion Method. A method to determine microbial
susceptibility to antibiotics in which filter paper disks containing known
concentrations of antibiotics are placed on the agar surface that has been
previously inoculated with the bacteria of interest (qeios.com). In this

study, it is a method used to test the antibacterial effect of the Tomato
Hand Sanitizer.
Ethyl Alcohol. Also known as Ethanol, is a colorless flammable
liquid that has a pungent taste (Merriam-Webster Dictionary). In the study,
it is an alcohol used to kill germs present in Tomato Hand Sanitizer.
Hand Sanitizer. A liquid or gel, typically one containing alcohol,
that is used to clean the hands and kill infection-causing microorganisms
(Oxford Languages). Specifically, it is the product produced using tomato.
Lycopene. A non-provitamin A carotenoid that is responsible for
the red to pink colors seen in tomatoes, pink grapefruit, and other foods. It
is also a dietary antioxidant known to prevent skin photodamage (Food
Science & Nutrition). Operationally, it is a substance in Tomato Hand
Sanitizer that prevents skin damage.
Sanitation. The promotion of hygiene and the prevention of
diseases by maintaining hygienic conditions (e.g. by disposing of sewage
and waste) (Merriam-Webster Dictionary). In this study, it is the promotion
of hygiene using Tomato Hand Sanitizer.
Skin Irritation. An irritation or inflammation of the skin due to an
allergy or infection caused by natural agents or by a skin test
(dictionary.com). In this research, it is specifically an irritation of skin

caused by other produced hand sanitizers that the researchers aim to
prevent.
Soothing. Producing feelings of comfort or relief (Merriam-Webster
Dictionary). As used in this research, it is when the skin looks smoother,
healthy, and radiant caused by the Tomato Hand Sanitizer.
Tomato. A glossy red, or occasionally yellow, pulpy edible fruit that
is eaten as a vegetable or in salad (Oxford Languages). Operationally, it is
a plant rich in substances that are good for the skin used in producing the
Vitamin C. A nutrient that the body needs in small amounts to
function and stay healthy (cancer.gov). As used in this study, it stimulates
collagen synthesis and assisting in antioxidant protection against UV-
induced photodamage (ncbi.nlm.nih.gov) that is included in the Tomato
Hand Sanitizer.
CHAPTER II
REVIEW OF RELATED LITERATURE AND STUDIES

This chapter presents the review of related and research literature,
and synthesis of the study.
Research Literature
The following review includes concept on: 1) Sanitation, 2) Tomato
Extract, 3) Soothing Effect, 4) Antibacterial Effect.
Sanitation. According to Center for Disease Control and
Prevention (CDC) (2021), the ability to maintain hygienic conditions is
considered to be an essential component of basic sanitation. Basic
sanitation is defined as having access to facilities for the safe disposal of
human waste. One of the most important methods for preventing infection
is maintaining good hand hygiene. Antibacterial hand sanitizer is
becoming more common due to their rapid action and effectiveness in
eradicating germs (Jing et al., 2020).
Tomato Extract. According to Direct Eats (2021), tomato extract
is lycopene-rich. Lycopene is a phytonutrient and a pigment compound
found in tomatoes, responsible for their red color and is loaded with health
benefiting properties. It is made by crushing tomatoes to produce tomato
juice that is then separated into serum and pulp.
Two experiments were performed to test the effects of rich tomato
extract on human skin. In one experiment, the effects of this extract on

gene expression in cultured human dermal fibroblasts were examined. In
a second experiment, human subjects consumed the extract and trans-
epidermal water loss (TEWL), and aspects of skin appearance were
monitored. Tomato extract may provide benefits to the skin by enhancing
innate immunity and other defense mechanisms in the dermis and by
providing antioxidants to the skin surface to optimize TEWL and the
appearance of the skin (Tarshish et al., 2022).
Soothing Effect. Dictionary.com defines soothing as a feeling of
ease, comfort, and calmness. Skin-soothing substances calms irritation or
inflammation on skin. It greatly works on sensitive skin such as eczema
and acne. However, studies have shown that it is applicable and effective
for all skin types (Barron, 2022).
Antibacterial Effect. According to Stoppler, MD (2021), anything
that eliminates bacteria or inhibits their development or capacity to
reproduce is considered antibacterial. Antibacterial qualities can be found
in heat, chemicals like chlorine, and antibiotic medications. Today, there
are many antibacterial cleaning and handwashing products available.
Such products do not lessen the likelihood that otherwise healthy people
would develop viral infectious disease symptoms. This does not rule out
the possibility that antibacterial products could help lessen bacterial
disease symptoms in the household.

Moreover, Soltani, et al. (2012) discussed that in the majority of
institutions and hospitals, the disk diffusion test is the commonly used
appropriate method for determining the antimicrobial susceptibility pattern.
Additionally, Horvath, et al. (2016) mentioned that disk diffusion method
(DDM) is categorized as an agar diffusion method (ADM) because the
plant extract to be tested diffuses from its reservoir through the agar
medium seeded with the test microorganism.
Related Studies
According to Bhowmik et al. (2012), tomato has a lot of benefits for
people’s health, specifically for the skin. Tomato is an exceptional plant
rich in lycopene, beta-carotene, antioxidants, and Vitamin E and C that is
good for the skin and rapid skin cell replacement. The study stated that
tomato had been used as a substance in making effective cleanser,
sunscreen, and different skincare products. It is suggested to leave the
sliced tomatoes on the skin for at least ten minutes to achieve a clean and
shiny skin.
In addition to that, Chayanika (2022) stated there are a number of
advantages to using tomato juice on the face. Regular tomato application
can assist in removing dead skin cells from the skin. This is due to
tomatoes' abundance of enzymes, which act as exfoliators naturally.

Additionally, you can use tomato and sugar as a mild face exfoliator.
Vitamins C, A, and K are abundant in tomatoes. To add to that, it is acidic,
which helps keep the pH of your skin while deeply cleansing it. It contains
a lot of vitamin C and vitamin A, which helps calm the skin and get rid of
the tan. Tomato paste contains a lot of lycopene, which protects against
sunburn. Tomatoes' anti-inflammatory component is beneficial for dry,
itchy skin. The main components of tomatoes that have a calming effect
on the skin are beta carotene, vitamin C, and vitamin E. This study
recommended that applying tomatoes regularly to skin that is prone to
breakouts of acne can help. It can also treat sunburns, helps you get rid of
tan lines, and reduce skin inflammation.
Costa et al. (2015) used an oral supplement that contains tomato
extract to manage skin aging among men. The 47 male participants in this
single-center, open-label, quasi-experimental clinical study ranged in age
from 30 to 45 and had Fitzpatrick phototypes I to IV. For 180 days in a
row, subjects took two tablets of the oral supplement. The study found that
tissue inflammation can be healed through the Lycopene and Vitamin C
present in tomato that also produce a powerful antioxidant. To sum up the
process, the experiment revealed that the oral supplement did not only
cause improvements in skin aging but also helped smoothens and
hydrates skin. Improvements in dermal density and facial skin hydration

imply that this product may have beneficial effects on skin aging. These
improvements are linked to a decrease in local pH and an increase in
collagen and elastic fibers. In this cohort of predominantly middle-aged
males, oral supplementation was demonstrated to be safe.
The study of Sopyan et al. (2018) developed a sunscreen lotion
using tomato extract. Because of the antioxidant lycopene, tomatoes
(Solanum lycopersicum L) are an effective source of sunscreen. The
outcomes revealed that the prepared sunscreen lotion had good physical
quality. However, the researchers suggested to store the produced
sunscreen lotion for 28 days only.
The innovation of new virus SARS-CoV-2 has presented
unprecedented difficulties to global public health. Currently, COVID-19
approaches are primarily supportive and preventative, with the goal of
minimizing transmission. Hand hygiene is an effective and concise
approach of minimizing illness transmission in public or healthcare
environments. Alcohol-based hand sanitizers have become a popular
alternative to traditional handwashing with soap and water in both primary
and secondary care. Alcohol-based hand sanitizers have been used as an
effective alternative to handwashing in preventing the spread of bacterial
and viral diseases, making it one of the most important procedures in
reducing healthcare burden (Golin et al., 2020).

Furthermore, the efficiency of orange as a bacterial hand sanitizer
had been studied by Bernabe et al. (2013). The researchers used orange
because of its rich Vitamin C and it is easily accessible, can be found
anywhere in the market. In addition to that, orange zest helps moisturize
the skin. The experiment concluded that the orange hand sanitizer is
effective because it contains Ethyl Alcohol that made it stronger against
germs and bacteria. The result lead to the recommendation of adding
more aloe vera, to make it more jelly-like agent.
Likewise, utilizing okra (Abelmoschus esculentus) and citrus fruits
as hand sanitizer was the title of a study by Rivera et al. (2019) that
sought to establish the efficacy of hand sanitizer in lowering bacterial
counts using okra and citrus fruits as main ingredients as well as to
determine whether the hand sanitizer is less irritating and fragrant to skin.
The respondents, who were students at Philippine Southfield School and
residents of barangay Bugo, examined samples of hand sanitizer.
Interviews were used as the study instrument and method of data
collection by the researchers. The product's texture was just adequate for
their hands to manage, according to the interview and the views of the
responders who used the hand sanitizer. They also noted that the
product's scent is unpleasant and that it doesn't feel greasy on the hands.
The respondents who have tested the product said that it was successful.
The study recommended that people or students can use this directly
because it has been demonstrated that okra has no negative side effects
and that using this hand sanitizer takes less time than washing your
hands. Furthermore, due to the ethyl alcohol utilized, using this product
determined its effectiveness in lowering bacterial counts on hands.
On the other hand, Yaun and Vasquez (2017) studied the
effectiveness of guava hand sanitizer against Staphylococcus aureus,
bacteria that can cause widespread illnesses. The researcher used the
Well Diffusion Method to test the antimicrobial susceptibility of the guava
hand sanitizer. The researchers compared the made-up guava hand
sanitizer to commercialized hand sanitizers. As the process and
experiments were done, it revealed that the guava hand sanitizer did not
have any significant difference to commercialized ones. This indicated that
the produced hand sanitizer using guava leaves extract was an effective
antibacterial.
In addition, the study of using the outer covering of a red onion was
conducted by Tolentino et al. (2016). To test the antibacterial effect of the
formulation, agar cup diffusion method was used by the researchers. The
study focused on comparing the formulated hand sanitizer against two
locally marketed hand sanitizers. The findings stated that the developed
hand sanitizer gel has more potent local antimicrobial action against the
two promoted hand sanitizer manufacturers in the Philippines and the
formulation was incapable of provoking skin irritation when rubbed on the
skin. The researchers recommended a thorough study about a particular
flavonoid that demonstrates antimicrobial activity.
Asumbrado (2021) created citrofortunella hand sanitizer using
calamansi and oranges. Calamansi and oranges are readily available in
the market, and some may be grown in the garden. Another reason the
researcher picked this study was because of its vitamin content. The
purpose of this study is to investigate the efficacy of citrofortunella hand
sanitizer as a soap and water alternative. Following the experiment, the
researcher concluded that the citrofortunella hand sanitizer was a definite
success due to the presence of ethyl alcohol, which made it more
disinfectant, and the ingredients used to make the hand sanitizer were
readily available and easily accessible in the market and stores. Upon the
trial, the researcher suggested adding more aloe vera and essential oil to
make the hand sanitizer more viscous and to have a pleasant smell. The
researcher also recommended utilizing food coloring for a better physical
appearance.
Synthesis
This section discusses the applicability of the studies examined as
well as the similarities and differences between the concepts scrutinized
and the current study.
In the article of Direct Eats, the beneficial substances on skin that
are present in tomatoes were discussed. In addition to that, the study of
Chayanika and Bhowmik et al. discussed the benefits of tomatoes to the
people's health, especially to the skin. Furthermore, Bhowmik et al.
suggested to leave the slices of tomato directly on skin. In association to
these concepts, this paper also used tomatoes because of the given
vitamins and beneficial substances present to it, such as lycopene, beta-
carotene, vitamins C and E. On the contrary, this study used tomato
extract combined to a particular amount of aloe vera and ethyl alcohol to
make a hand sanitizer.
In the study of Costa et al., it was showed that an oral supplement
containing tomato extract resulted to a success experiment. The oral
supplement was tested effective in managing skin aging and helps
smoothens and hydrates the skin as well. In addition, Sopyan et al.
formulated a sunscreen lotion using tomato extract which proven effective
against ultraviolet rays due to the presence of antioxidant in tomatoes. In
relevance to the study of Costa et al. and Sopyan et al., this research aims
to produce a product using tomato extract that soothes the skin. In

contrast, this paper is intended on formulating an antibacterial hand
sanitizer.
Soltani et al. discussed that disk diffusion method is the frequently
used method to test the antimicrobial susceptibility pattern. In accordance
to this concept, this study will use the disk diffusion method to determine
the efficiency of the produced hand sanitizer in terminating germs and
bacteria.
Health professionals have stated clearly that handwashing with
hand sanitizer is one of the most efficient methods of avoiding the
transmission of germs and viruses (CDC). The study of Golin et al. and
Stoppler MD focused on the use of alcohol-based hand sanitizers as an
effective alternative to handwashing in avoiding the spread of bacterial
and viral infections and lowering healthcare costs. In relation, this
research considered the impact of Covid-19 in producing a hand sanitizer.
In contrast, the researchers of this study propose to use the tomato plant
to create an antibacterial, skin soothing hand sanitizer.
Many studies developing hand sanitizer had already been done.
One of it is Rivera et al. Where they used okra and citrus fruits to
formulate an effective antibacterial hand sanitizer. They tested their
produced hand sanitizer to the students and the instrument they used in
data collection was an interview. Meanwhile Yaun and Vasquez used
guava in formulating an effective hand sanitizer against Staphylococcus
aureus, a kind of bacteria. Another one is Tolentino et al. where they
used a red onion. Additionally, an orange was employed in the study of
Bernabe et al. as hand sanitizer because it includes vitamin C and
orange zest that moisturizes the skin. Similarly, citrofortunella by
Asumbrado used calamansi and oranges as a hand sanitize for the
reason that calamansi and oranges are simple to get in the market or
garden.
The current paper aims to formulate a hand sanitizer that can be
used against bacteria similar to the aim of the aforementioned studies
above. Yet none of them had use tomato which is the main focus of the
current study. Unlike Rivera et al. who used interview to test the
effectiveness of their product the current research will utilize
experimental test specifically disk diffusion method to test the
antibacterial property. This method was proven effective in the study of
Yaun and Vasquez and Tolentino et al.
Conceptual Framework
Input
1. Tomato Extract
Process Output
1. Distributing the
particular amount
of tomato extract, Tomato Hand
ethyl alcohol, and Sanitizer
aloe vera in
different
concentrations.
2. Disk Diffusion
Method
3. Examining the
soothing effect,
acidity, and lifespan
of the produced
hand sanitizer.
4. Comparing the
smell, color, and
texture of the hand
sanitizer.
5. Analyzing the
data gathered.
Figure 1 shows the input of the study. This includes the tomato
extract, ethyl alcohol, aloe vera, level of anti-bacterial effect, soothing
effect, acidity, and lifespan of the hand sanitizer, as well as its smell, color,
and texture.
Figure 2 illustrates the process which includes evaluating the
needed amount of tomato extract, ethyl alcohol, and aloe vera, disk
diffusion method, examining the soothing effect, acidity, and lifespan of
the produced hand sanitizer, comparing the smell, color, and texture of the
hand sanitizer, and analyzing the data gathered.
Figure 3 is the output which contains the tomato hand sanitizer.

CHAPTER III
METHODOLOGY
This chapter presents the research design, research environment,
respondents/subject of the study, data gathering procedures as well as the
statistical treatment of data.
Research Design
In this study, the researchers used an experimental research
design. According to Sirisilla (2022), experimental research design is a
framework that used a scientific approach between two sets of variables,
the independent and dependent variable. To assess the efficiency of
tomato as hand sanitizer for soothing skin, this method is the most
suitable to use.
Research Environment
The study was conducted at Calatagan, Batangas. Producing the
hand sanitizer as well as testing its antibacterial and soothing effect and
acidity level were conducted at the Science Laboratory of Calatagan
Senior High School.

Materials and Methods
A. Materials and Equipment
The materials used for the product are as follows: ripe tomatoes,
aloe vera, ethyl alcohol, water, we also need a blender, a clean cloth, 2
bowls, spoon, funnel, and small containers. To identify the effectiveness of
the hand sanitizer, the following instrument will be used: pH test strips, 3
apples, and disk diffusion test.
B. Methodologies
b1. Extracting Tomato
First, ripe tomatoes were collected and washed. Second, peel the
skin of the tomatoes using a knife and put the seedless and peeled
tomatoes in the blender. Then, strain the blended tomato using a clean
cloth and set aside after it is done.
b2. Preparing the Aloe Vera
Gather and wash the aloe vera. Next, peel the skin of the aloe vera
using a knife. After peeling its skin, squeeze the aloe vera using a fork in a
bowl to acquire the jelly texture and set it aside in a different container.
b3. Making the Hand Sanitizer
Combine the indicated amounts of alcohol, tomato extract, and aloe
vera gel in the small containers.
b4. Solutions
The following solutions were followed during the experiment:
Treatment A 0% Tomato Extract, 50% Ethyl Alcohol, and 50%
Aloe Vera
Treatment B 20% Tomato Extract, 40% Ethyl Alcohol, and 40%
Aloe Vera
Treatment C 30% Tomato Extract, 35% Ethyl Alcohol, and 35%
Aloe Vera
Treatment D 40% Tomato Extract, 30% Ethyl Alcohol, and 30%
Aloe Vera
Treatment E 50% Tomato Extract, 25% Ethyl Alcohol, and 25%
Aloe Vera
b5. Checking the Acidity Level
A pH test strip is a strip of litmus paper used to determine the pH of
a liquid. At different acidities, the material in the paper causes the paper to
change color. This test will be used by the researchers to determine the
acidity of the produced sanitizer. The official pH scale ranges from 0 to 14,
with 0 being extremely acidic and 14 being extremely alkaline.
b6. Checking the Soothing Effect
Three apples will be used to test the soothing effect of the
produced hand sanitizer. Each apple will be sliced in half. The first half of
the apple will be set aside, after an hour the researchers will capture the
apple and its color will serve as the basis of the other slices with
treatments. The remaining sliced apples will be labeled A, B, C, D, and E.
The corresponding treatments will be applied on each slice. The timer will
be set until the apple turns into as brown as the first slice. The longer the
time, the more effective the hand sanitizer is in terms of soothing effect.
b7. Checking the Antibacterial Effect
To check for the antibacterial effect using the Disk Diffusion
Method, take an antibiotic disk with tweezers, dip it in hand sanitizer, and
place it in the incubator. Repeat these steps to other treatments. Three
trials of this method will be conducted in order to compare them and
determine which is the most effective.
b8. Checking the Lifespan
The researchers will utilize the Accelerated Shelf-life Testing,
putting it in a humid area, to ascertain the shelf-life of the hand sanitizer
and make predictions about it. Additionally, this will be used to determine
how to keep the hand sanitizer in the best possible condition.

C. Workplan
MATERIALS COLLECTION
CALCULATION OF DIFFERENT CONCENTRATIONS
TESTING OF THE PRODUCT
Treatment A: Treatment C:
0% Tomato 30% Tomato
Extract, 50% Extract, 35%
Ethyl Alcohol, Ethyl Alcohol,
50% Aloe Vera 35% Aloe Vera
Treatment D: Treatment B: Treatment E:

40% Tomato 20% Tomato 50% Tomato
Extract, 30% Extract, 40% Extract, 25%
Ethyl Alcohol, Ethyl Alcohol, Ethyl Alcohol,
30% Aloe Vera 40% Aloe Vera 25% Aloe Vera
a. Acidity e. Smell
b. Soothing Effect f. Color
c. Antibacterial Effect h. Texture
d. Lifespan
ANALYSIS OF RESULT
Data Gathering Instrument
The instruments used by the researchers in acquiring the needed
information are through scientific experiments and observation. The
researchers used an apple to test the soothing effect of the hand sanitizer,
Disk Diffusion Method to determine its antibacterial effect, pH Test Strip to
assess its acidity level. The evaluation was done on the produced hand
sanitizer using tomatoes that was executed in the laboratory. While the
plant growth was done in the field.
Scoring. Data from the observation checklist will be collected,
tabulated, and interpreted. The researchers will use Likert Scale Rating to
rate the color, smell, and texture of the produced hand sanitizer. The
following information will be interpreted by the 4-point Likert Scale.
Smell
Options Range Verbal Interpretation
4 3.51 – 4.00 Very Strong Smell
3 2.51 – 3.50 Strong Smell
2 1.51 – 2.50 Mild Smell
1 0.50 – 1.50 Very Mild Smell

Color
4 3.51 – 4.00 Red
3 2.51 – 3.50 Tart
2 1.51 – 2.50 Orange-red
1 0.50 – 1.50 Light Coral
Texture
4 3.51 – 4.00 Very Viscous
3 2.51 – 3.50 Viscous
2 1.51 – 2.50 Dilute
1 0.50 – 1.50 Very Dilute
Statistical Treatment of Data
The data gathered in the study employed the following statistics:
Amount of tomato extract, ethyl alcohol, and aloe vera in the

produced hand sanitizer
Percentage were the basic statistical tools used to determine the

different measurement of tomato extract, ethyl alcohol, and aloe vera as
hand sanitizer.
Antibacterial effect of the produced hand sanitizer

Weighted mean and ranking were used to determine the

antibacterial effect of the produced hand sanitizer in terms of 3 test trials.
Significant difference in acidity level, soothing effect,

antibacterial effect lifespan, color, smell, and texture of the produced
hand sanitizer among different concentrations
ANOVA will be used to determine the significant difference among

the Acidity Level, Soothing Effect, Lifespan, Color, Smell, and Texture of
the hand sanitizer in different concentrations which are all interval data.
Kruskal-Wallis test will be used to determine the significant difference in
Antibacterial Effect of the hand sanitizer in different concentrations which
is ordinal data.
.
APPENDIX A
OBSERVATION CHECKLIST
Indicators Control A B C D Observational

Notes
1. The amount of
tomato extract in
the produced hand
sanitizer.
2. The amount of
ethyl alcohol in the
produced hand
sanitizer.
3. The amount of
aloe vera in the
produced hand
sanitizer.
4. The pH level of
the produced hand
sanitizer.
5. The produced
hand sanitizer
prevents the darken
of the apple.
6. The Trial 1
produced
hand
sanitizer Trial 2
is an
effective
antibacter Trial 3
ial
substance
.
7. The shelf life of

the produced hand
sanitizer.
8. The
1
smell of
the
produced 2
hand
sanitizer. 3
9. The 1
redness
of the
2
produced
hand
3
sanitizer.
10. The 1
texture of 2
the
produced
3
hand
sanitizer.
4
CURRICULUM VITAE
PERSONAL DATA
Name : Eduardo L. Ilang Ilang
Address : Balibago, Calatagan, Batangas
Place of Birth: Bacoor City, Cavite
Date of Birth : November 18, 2004
Civil Status : Single

Educational Background
Elementary : Balibago Elementary School
Balibago, Calatagan, Batangas
2011 – 2017
Junior High School : Lucsuhin National High School
Lucsuhin, Calatagan, Batangas
2017 - 2021
Senior High School : Calatagan Senior High School
2021 - present
CURRICULUM VITAE
PERSONAL DATA
Name : Ricardo A. Ilao Jr.
Address : Sta. Ana, Calatagan, Batangas
Place of Birth: Sta. Ana, Calatagan, Batangas
Date of Birth : November 28, 2003
Elementary : Pedro Palacio Memorial Elementary School
Sta. Ana, Calatagan, Batangas
2011 - 2017
Junior High School : Calatagan National High School
2017 - 2021
2021 - present
CURRICULUM VITAE
PERSONAL DATA
Name : Althea A. Aytona
Address : Tanagan, Calatagan, Batangas
Place of Birth: Tanagan, Calatagan, Batangas
Date of Birth : September 08, 2005
Elementary : Tanagan Elementary School
Brgy. Tanagan, Calatagan, Batangas
2011- 2017
2017 - 2021
2021 – present
CURRICULUM VITAE
PERSONAL DATA
Name : Joyce C. Bernal
Address : Poblacion 4, Calatagan, Batangas
Place of Birth: Calatagan, Batangas
Date of Birth : December 17, 2004
Elementary : Jacobo Zobel Elementary School
2011 - 2017
2017 - 2021
2021 - present
CURRICULUM VITAE
PERSONAL DATA
Name : Jhervie P. Morales
Address : Carretonan, Calatagan, Batangas
Place of Birth: Calatagan, Batangas
Date of Birth : April 28, 2005
Elementary : Angelita Zobel Elementary School
2011 - 2017
Junior High School : Lucsuhin National High School
2017 - 2021
2021 - present

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Calatagan Senior High School

EFFECTIVENESS OF TOMATO AS HAND SANITIZER

Eduardo L. Ilang Ilang

1. THE PROBLEM AND THE BACKGROUND

Background of the Study..............................................................................

Statement of the Problem.............................................................................

Significance of the Study..............................................................................

Scope, Limitation and Delimitation...............................................................

2. REVIEW OF RELATED LITERATURE AND STUDIES

Respondents/Subject of the Study...............................................................

Data Gathering Procedure............................................................................

Statistical Treatment of Data........................................................................

THE PROBLEM AND THE BACKGROUND

Background of the Study

activities without proper sanitation. According to World Health

Organization, from January 2020 to September 2022, there are almost 4

million confirmed cases in the Philippines, including the fatalities and

different diseases and sickness, such as common cold and influenza. To

alcohol can be use (CDC, 2021). However, according to a study, habitual

use of this may result to dryness and irritation of skin. In agriculture,

tomato production had been a problem in the Philippines. Tomatoes were

thrown away by the farmers due to oversupply in some areas of our

country since the pandemic outbreak (GMA News Online, 2022).

Ripe tomato is a plant rich in Vitamin C, Lycopene, and beta-

Carotene. Vitamin C hydrates, brightens, and repairs damaged skin. In

Carotene is an antioxidant that keeps the appearance and healthiness of

the skin (Ebanel, 2020). Some well-known manufacturers of sunscreens,

one of their main ingredients because of its benefits on skin.

From the cited concern on existing hand sanitizers and benefits of

tomato, the researchers seek to develop an antibacterial and at the same

Statement of the Problem

This study aims to develop an alternative soothing hand sanitizer

using a tomato plant that prevents skin irritation.

Specifically, this study seeks answers to the following questions:

1. What is the amount of:

1.1 Tomato Extract,

1.2 Ethyl Alcohol, and

1.3 Aloe Vera in different concentrations?

2. What is the outcome of Tomato Hand Sanitizer in terms of:

2.2 Soothing Effect,

2.3 Antibacterial Effect, and

2.4 Lifespan in different concentrations?

3. What is the appearance of the produced hand sanitizer in terms

3.2 Color, and

3.3 Texture in different concentrations?

4. Is there a significant difference in acidity level, soothing effect,

antibacterial effect, lifespan, smell, color, and texture of the produced

hand sanitizer among different concentrations?

5. What process can be done to enhance the effectiveness of

produced hand sanitizer?

Significance of the Study

The study entitled, “Effectiveness of Tomato as Hand Sanitizer for

Soothing Skin” will be significant to the following:

Environment. This study will be beneficial to the environment as it

affordable ingredients causing its price to be affordable as well, and will

help keep a healthy environment that is skin-friendly at the same time. It

at malls, schools, especially in health care institutions.

Students. This research will be mainly beneficial to Science,

Technology, Engineering, and Mathematics (STEM) students for the

can use it.

Future Researchers. The findings of this study will serve as a

guide for future researchers undertaking comparable study. Future