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Biochem Questions
Biochem Questions
COLLEGE OF MEDICINE
DEPARTMENT OF BIOCHEMISTRY
OBJECTIVE:
A. The students must be able to describe the chemical nature and properties
of the different types of lipids and relate them to their cellular functions
2. . In the shuttle of mitochondrial acetyl coenzyme A to the cytosol for fatty acid
synthesis, in which step generates NADPH?
a. oxaloacetate + acetyl coenzyme A citrate
b. oxaloacetate malate
c. malate pyruvate
d. pyruvate oxaloacetate
e. citrate _ coenzyme A oxaloacetate _ acetyl coenzyme A
( Harper’s ch 24 pp238-239)
3. The lipoprotein that serves to transport triacylglycerol from the liver to the
different extrahepatic tissues:
a. chylomicrons
b. high density lipoproteins
c. intermediate density lipoproteins
d. low density lipoproteins
e. very low density lipoproteins
(Harper’s Ch 27 p 268-271)
Page 1 of 17
5. . In the following steps in ketone bodies metabolism, NADH is generated in:
a. acetoacetate acetone
b. acetoacetate beta hydroxybutyrate
c. acetoacetyl coenzyme A + acetyl coenzyme A HMG coenzyme A
d. beta hydroxybutyrate acetoacetate
e. HMG coenzyme A acetoacetate + acetyl coenzyme A
( Harper’s ch 24 p 244-245)
10. . Ketone bodies maybe synthesized from fatty acids by which of the following
organs or cells?
a. skeletal muscle
b. liver
c. kidney
d. erythrocytes
e. brain
(Harper’s ch 24 p242)
Page 2 of 17
11. Spontaneous decarboxylation of acetoacetate results in the formation of:
a. acetone
b. acetyl coenzyme A
c. 3-hydroxybutyrate
d. malonyl coenzyme A
e. propionate
( Harper’s ch 24 p 242-243)
12. Which of the lipoproteins has the highest total lipid content?
a. chylomicrons
b. high density lipoproteins
c. intermediate density lipoproteins
d. low density lipoproteins
e. very low density lipoproteins
( Harper’s p 268)
15. Of the following lipids, which one is accumulated is tissues of patient with
Tay-Sach’s Disease?
a. ceramide trihexoside
b. galactocerebroside sulfate
c. ganglioside
d. glucoceberoside
e. sphingomyelin
(Harper’s p 267)
Page 3 of 17
18. In the biosynthesis of fatty acids, what is the compound that transports the
acetate group out of the mitochondria into the cytoplasm
a. pyruvate
b. malate
c. citrate
d. acyl carnitine
e. acyl coenzyme A
(Harper’s ch 23 p 236)
19. Activation of medium chain and short fatty acids occurs in the
a. Ribosomes
b. Mitochondrial matrix
c. Golgi apparatus
d. Endoplasmic reticulum
e. Cytosol
(Harper’s pp 238-239)
OBJECTIVES:
a) The student must be able to describe adequately the chemical properties
of carbohydrates and relate them to their cellular function
Page 4 of 17
3. Of the following hexoses, which is a constituent of glycolipids and
glycoproteins?
a. glucose
b. galactose
c. fructose
d. xylulose
( Harper’s ch 56 p 676-677)
Page 5 of 17
10. In humans, liver glycogen stores are adequate up to how many hours
without support from gluconeogenesis?
a. 4 hours
b. 12 hours
c. 24 hours
d. 48 hours
(Harper’s pp214-217)
OBJECTIVES:
1. The students must understand the energy transformations known
to occur in cells
5. In the complete oxidation of one (1) mole of palmitic acid to CO2 and H2O, the
total number of ATP generated is
a. 12
b. 24
c. 36
d. 129
e. 131
(Harper’s Ch 23)
Page 6 of 17
6. Synonymous with Kreb’s cycle
a. Pentose phosphate pathway
b. Citric acid cyle
c. Oxidative phosphorylation
d. Embden myerhoff pathway
(Harper’s ch 18 p 182f)
7. Anaerobic glycolysis produces how many moles of ATP per mole of glucose?
a. 2
b. 6
c. 8
d. 12
e. 24
(Harper’s ch 19 p 190-191)
OBJECTIVES:
1. The student must be able to describe the chemical structure
and properties of proteins and amino acids
1. Which of the following amino acid functional group pairs is not correct
a) arginine:guanidine
b) histidine:imidazole
c) phenylalanine: hydroxyphenyl
d) tryptophan : indole
e) cysteine : sulfhydryl
(Harper’s p 29)
Page 7 of 17
2.Helical formation or Helix is a characteristic of what protein structure?
a. primary
b. secondary
c. tertiary
d. quaternary
e. all of the above
(Harper’s p 49)
3. Which of the following amino acids serve as the major mode of disposing
ammonia from the brain?
a. alanine
b. glutamine
c. threonine
d. serine
e. valine
(Harper’s p 308/317)
4. Among the following amino acids, which one is converted to pyruvate through
transamination reaction?
a. alanine
b. methionine
c. threonine
d. valine
( Harper’s pp315-316)
Page 8 of 17
8. Urea is the major pathway of nitrogen excretion in humans. It is synthesized in
the:
a. brain
b. intestine
c. kidney
d. liver
e. skeletal muscle
( Harper’s pp315…)
9. Among the intermediates in urea cycle, which one provides the linkage of urea
cycle and the citric acid cycle?
a. Alpha ketoglutarate
b. aspartate
c. fumarate
d. malate
e. oxaloacetate
(Harper’s pp319-321)
14. Which of the following amino acids contain indole functional group?
a. Arginine
b. Histidine
c. Phenylalanine
d. Tryptophan
e. Tyrosine
(Harper’s p 29)
Page 9 of 17
15. A biochemical technique used to separate molecules based on both size and
charge
a. gel electrophoresis
b. spectrophotometry
c. ultracentrifugation
d. paper chromatography
( Harper’s P48/53)
16. Which of the following enzymes funnel amino nitrogen from glutamate to
urea?
a. glutamate transaminase
a. alanine transaminase
b. amino acid oxidase
c. glutamate dehydratase
d. glutamate dehydrogenase
(Harper’s pp319-321)
18. The metabolism of the following amino acid leads to the production of small
amounts of nicotinic acid in humans
a. cysteine
b. methionine
c. serine
d. tryptophan
e. valine
( Harper’s chapter 42)
OBJECTIVES:
1. The student should be able to describe the structural organization
of nucleosides, nucleotides and nucleic acids and relate them to
their cellular functions
Page 10 of 17
2. The students should discuss and understand the
biochemical basis of diseases resulting from impairment in
the biosynthetic and catabolic pathways of purine and
pyrimidine nucleotide metabolism
Page 11 of 17
7. A PURINE BASE
a. cytosine
b. guanine
c. uracil
d. thymine
( HARPER’s ch 37 p 402)
E. ENZYMES
OBJECTIVES:
Page 12 of 17
2. Group of enzymes that catalyze the addition or removal of water, ammonia or
carbon dioxide to double bonds:
a. hydrolases
b. isomerases
c. ligases
d. lyases
e. oxidoreductases
Page 13 of 17
9. This enzyme is valuable in the diagnosis of metastatic carcinoma of the
prostate gland.
a. ceruloplasmin
b. alkaline phosphatase
c. lactate dehydogenase
d. acid phosphatase
e. aspartate aminotransferase
10. A group of enzymes that join two molecules along with breakdown of a
pyrophosphate (P-P) bond
a. ligase
b. isomerase
c. lyase
d. transferase
Page 14 of 17
5. Site of aldosterone secretion in the adrenal cortex:
a. zona fasciculata
b. zona granulosa
c. zona intima
d. zona pellucida
e. zona reticularis
8. In the adipose tissue, which of the following hormones enhances the synthesis
of lipoprotein lipase?
a. cortisol
b. epinephrine
c. glucagons
d. insulin
e. progesterone
( Harper’s p 246)
Page 15 of 17
E. VITAMINS AND MINERALS
OBJECTIVES:
1. The students must understand clearly the nutritional aspects of vitamins
and minerals and appreciate their role in the maintenance of normal
body functions
2. The students must correlate the clinical disorders that will result from
the deficiency and excesses of these vitamins with their metabolic roles
in the body.
Page 16 of 17
6. The earliest clinical symptom of Vitamin A deficiency
a. defective night vision
b. xerophtalmia
c. presence of Bitots spots
d. Keratomalacia
e. Xerosis conjunctivae
(Harper’s p 643)
Page 17 of 17