SECOND WRITTEN TEST IN EARTH AND LIFE SCIENCE

QUARTER 2, SY 2022-2023

Instructions: Read each question carefully and write the correct answer in a separate
sheet of paper, or in the answer sheet provided to you. Do not write anything on this
test questionnaire.

1. What term refers to the group of techniques used to cut up and join together
genetic material, especially DNA from different biological species?
A. cloning
B. gene therapy
C. genetic engineering
D. DNA fingerprinting

2. Given the situation: “John is suspecting that Jose was his long-lost father. He
wants to verify if they were blood related and is thinking of rDNA technology in proving
his suspicion”. If you are to give suggestion to John of rDNA technology application,
what would it be?
A. Apply biosynthesis of interferon.
B. Use DNA fingerprinting and compare your DNA profile.
C. Apply gene therapy and ensure the restoration of normal cellular activity.
D. Use the production of hormones and compare if you have the same number of
hormones.

3. Which of the following is TRUE about the advantages of asexual reproduction?

A. Fission in corals produces clones that does not allow genetic variation.
B. Colonies of hydra produced through budding have no improvement in their
characteristics.
C. Regeneration in starfish helps them survive when they encounter a greater number
of predators.
D. Parthenogenesis in bees produces no movement of genes from one population to
another enabling them not to adapt to unstable environments.

4. Humans, like other animals, undergo a process in reproducing its offspring. The
following are the process involved in the formation and development of human
embryo.
I. fertilization
II. blastocyst development
III. blastocyst implantation
IV. embryo development
V. fetal development

What is the correct order in the formation of human fetus?

A. I, III, II, IV, V
B. I, II, III, V, IV
C. I, II, III, IV, V
D. I, IV, III, II, V
5. Analyze the different steps below which involved genetic engineering or recombinant
DNA technology. What is the correct arrangement of steps in genetic engineering?

I. Selection of Transformed Host Cells

II. Selection of Suitable Cloning Vector
III. Selection and Isolation of DNA Insert
IV. Expression and Multiplication of DNA Insert in the Host
V. Introduction of Recombinant DNA Molecule to a Suitable Host
VI. Introduction of DNA-insert into Vector to form rDNA Molecule

A. II, I, III, V, VI, IV

B. II, III, I, VI, V, IV
C. III, II, VI, V, I, IV
D. III, II, I, VI, V, IV

6. Golden rice is one of the examples of a genetically modified crop which contains
more Vitamin A than conventional rice. What conclusion could you formulate about
the benefits we can get from golden rice?
A. Golden rice has more Vitamin A but could not solve night blindness.
B. Because of golden rice number of people going blind had increased.
C. Golden rice consumption is safe which can solve Vitamin A deficiency.
D. Golden rice consumption doesn’t solve our problem with Vitamin A deficiency.

7. Hydra, coral, starfish and goldfish are aquatic animals that reproduces its offspring
in different methods. Which offspring will be MOST different from its parent?
A. coral
B. goldfish
C. hydra
D. starfish

8. Animals develop its fertilized eggs differently. Which of the following is TRUE about
the development of an animal embryo?
A. Animals that lay eggs are ovoviviparous.
B. Animals that give birth to offspring are viviparous.
C. Animals that lay eggs and develop the eggs inside the mother’s body are
ovoviparous.
D. Animals that lay eggs and develop its embryo outside the mother’s body are
oviparous.

9. Your aunt is diagnosed of having Type I diabetes, a condition in which the body
does not make insulin and cannot control the amount of sugar in the blood. What
rDNA technology could help your aunt solve her condition?
A. production of vaccines
B. production of hormones
C. production of antibiotics
D. biosynthesis of interferon
10. Evaluate the sets of animals with its corresponding reproduction process given
below. Which of the following has an incorrect combination?
A. Sponge-regeneration
B. lizard-parthenogenesis
C. catfish-external fertilization
D. guppy-viviparous internal fertilization

11. Enzymes are one of the tools used in rDNA technology. The statement: “enzymes
are proteins that recognizes a specific, short nucleotide sequence and cuts the RNA
only at specific site”, is false. What makes the statement incorrect?
A. Enzymes are fats that cut DNA only at specific site.
B. Enzymes are proteins that cut RNA only at specific site.
C. Enzymes are proteins that cut DNA only at specific site.
D. Enzymes are carbohydrates that cut RNA only at specific site.

12. Which of the following terms refers to the biological process of producing offspring
that are biologically or genetically similar to the parents?
A. evolution
B. fertilization
C. development
D. reproduction

13. Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-

2 virus that affects the lives of everybody around the world. What rDNA technology
could help solve our problem about COVID-19?
A. production of vaccines
B. production of hormones
C. production of antibiotics
D. biosynthesis of interferon

14. Consider the statement: “GM foods are good for human consumption since it has a
potential to develop allergens and toxicity”. How will you classify the statement?
A. The statement is correct but we should not consume GM foods.
B. The statement is correct, hence, there is no problem in consuming GM foods.
C. The statement is incorrect; thus, we need to stop consuming GM foods especially
that GM foods are not good to our health.
D. The statement is incorrect, hence, there is a need to support with recent findings
and studies the benefits and risks of consuming GM foods.

15. Which of the following crop is not genetically modified?

A. Bt corn
B. golden rice
C. BT eggplant
D. conventional rice
16. Which of the following is considered a risk of GM food to people’s health?
A. GM food enhanced pathogenicity.
B. GM food production cost is reduced.
C. GM food increased weed or pest burden.
D. GM food contains higher amounts of vitamins and minerals.

17. In a laboratory experiment, your teacher instructed you to cut starfish into pieces.
Several weeks later, you observed that each piece develops into a new starfish. Which
type of asexual reproduction is being described from the situation?
A. fission
B. budding
C. fragmentation
D. parthenogenesis

18. Your mother is one month pregnant and you noticed that she is still smoking. You
tried to remind her and explained to her that the life of your younger sibling is at risk.
After learning the process of reproduction and its importance, how will you explain to
your mom the risks she could get when she ingests toxins such as nicotine and
alcohol?
A. These toxins can enter the embryo when it eats.
B. These toxins can enter her uterus through her navel.
C. These toxins can diffuse from her blood to the embryo’s blood within the placenta.
D. These toxins transfer to the embryo since her blood normally mixes with the
embryo’s blood in the placenta.

19. What term refers to the process of producing new organism by combining the
genetic information of two individuals having different sexes?
A. external fertilization
B. internal fertilization
C. sexual reproduction
D. asexual reproduction

20. Which of the following statements correctly differentiate sexual reproduction from
asexual reproduction?
A. Sexual reproduction is faster than asexual reproduction.
B. Sexual reproduction requires one parent while asexual requires two.
C. Sexual reproduction requires two parents while asexual requires one.
D. Sexual reproduction produces identical offspring while asexual produces offspring
from two parents.

21. The following animals exhibit different types of reproduction processes. Which of
the following animals reproduce its offspring asexually?
A. A chicken egg that is fertilized by a rooster.
B. A frog releases its sperm and egg in water.
C. A starfish that reproduces by breaking off and generating parts of its body.
D. A red-tailed green rat snake develops its eggs inside the mother’s body but its
embryo obtains nourishment from egg yolk.
22. Rice is a common staple food that can fight hunger but lack many micronutrients.
Genetically engineered rice which is rich in micronutrients was developed to provide
staple food for everybody. Which do you think benefits you MOST in consuming
genetically engineered rice?
A. The need for pesticides and insecticides were reduced because of genetically
engineered rice.
B. There is a greater food security and medical benefits because of genetically
engineered rice.
C. Genetically engineered rice contains more Vitamin A which can help reduce the
number of people going blind.
D. Genetically engineered rice matures faster, tolerate environmental stressors, and
grows in the place where they are not used to flourish.

23. An expert farmer visits your community introducing the benefits of Bt corn. He
gives free Bt corn plants and advised the group of farmers in your community
including your father to plant Bt corn in your farm or land. Since you have learned the
benefits and risks of GMOs in your science class, what best advise could you suggest
to your father?
A. Bt corn is toxic especially to the monarch caterpillar.
B. Planting Bt corn is not good especially that it is harmful to some pestiferous
insects.
C. Planting Bt corn in your land can enhanced the ability of microorganisms to cause
damage to its host.
D. Bt corn is rich in protein which means reduction in the amount of insecticide to be
applied in crops.

24. Your family visit a beach where varied species of animals are found like starfishes
and different kinds of fish. Your younger sister asked you why starfishes looked the
same while the other fishes looked uniquely different from each other. Which of the
following is the BEST explanation you can give to your sister’s question?
A. Both the starfishes and other fishes reproduced sexually.
B. Both the starfishes and other fishes reproduced asexually.
C. The starfishes reproduced asexually while the other fishes reproduced sexually.
D. The starfishes reproduced sexually while the other fishes reproduced asexually.

25. Which of the following animals fertilized its gametes internally and develop its
embryo by receiving all the nutrients from the yolk content of the egg sacs?
A. birds
B. turtles
C. sharks
D. dolphins

26. The chances of survival among animals are greatly affected by its type of
reproduction and animal embryo development. Which of the following animals have
more chances of survival?
A. Philippine frog
B. Philippine duck
C. Philippine flying lemur
D. Philippine wild chicken
27. Which of the following is involved in the construction of recombinant DNA?
A. cleaving and rejoining DNA segments with ligase
B. cleaving and joining of DNA segments with endonuclease
C. cleaving DNA segments with endonuclease and rejoining with ligase
D. cleaving DNA segments with ligase and rejoining with endonuclease

28. Which of the following is TRUE about the benefits of genetically modified crops?
A. Genetically modified crops are more nutritious than conventional crops.
B. Genetically modified crops are a known health risk than conventional crops.
C. Genetically modified crops offer substantial health advantages than conventional
crops.
D. Genetically modified crops are neither better now worse than foods from
conventional crops.

29. Which of the following is NOT correct about recombinant DNA technology?
A. Plasmids and bacteriophages are examples of commonly used vectors.
B. Recombinant DNA (rDNA) technology helps produce better breeds of plants and
animals.
C. The rDNA technology could also help in identifying criminals by studying a sample
from a specific person.
D. The rDNA technology can be considered successful even if the foreign DNA inserted
into the vector DNA is not expressing the desired trait in the host cells.

30. Chicken laid their eggs outside their body wherein these eggs developed by
receiving nourishment from its yolk. Which of the following BEST describe the
fertilization of chickens?
A. Chickens are oviparous that fertilized its eggs internally.
B. Chickens are viviparous that fertilized its eggs externally.
C. Chickens are oviviparous that fertilized its eggs externally.
D. Chickens are ovoviviparous that fertilized its eggs internally.