19.09.

2022, 19:52 Sulforaphane and erucin increase MRP1 and MRP2 in human carcinoma cell lines - ScienceDirect

The Journal of Nutritional Biochemistry

Volume 19, Issue 4, April 2008, Pages 246-254

Research article

Sulforaphane and erucin increase MRP1 and MRP2 in human

carcinoma cell lines
Kristin E. Harris, Elizabeth H. Jeffery

Show more

Outline Share Cite

https://doi.org/10.1016/j.jnutbio.2007.02.014 Get rights and content

Abstract
Multidrug resistance (MDR) transporters have been termed the Phase III detoxification system because
they not only export endogenous metabolites but provide protection from xenobiotic insult by actively
secreting foreign compounds and their metabolites from tissues. However, MDR overexpression in tumors
can lead to drug resistance, a major obstacle in the treatment of many cancers, including lung cancer.
Isothiocyanates from cruciferous vegetables, such as sulforaphane (SF) and erucin (ER), are known to
enhance the expression of Phase II detoxification enzymes. Here we evaluated the ability of SF and ER to
modulate MDR mRNA and protein expressions, as well as transporter activity. The expression of P-
glycoprotein (P-gp), multidrug resistance protein 1 (MRP1) and multidrug resistance protein 2 (MRP2) in
liver (HepG2), colon (Caco-2) and lung (A549) cancer cells treated with ER or SF was analyzed by Western
blotting. Neither SF nor ER affected P-gp expression in any of the cell lines tested. Both SF and ER
increased the protein levels of MRP1 and MRP2 in HepG2 cells and of MRP2 in Caco-2 cells in a dose-
dependent manner. In A549 lung cancer cells, SF increased MRP1 and MRP2 mRNA and protein levels; ER
caused a similar yet smaller increase in MRP1 and MRP2 mRNA. In addition, SF and ER increased MRP1-
dependent efflux of 5-carboxyfluorescein diacetate in A549 cells, although again the effect of SF was
substantially greater than that of ER. The implication of these findings is that dietary components that
modulate detoxification systems should be studied carefully before being recommended for use during
chemotherapy, as these compounds may have additional influences on the disposition of chemotherapeutic
drugs.

Previous Next

Keywords
Sulforaphane; Erucin; Multidrug resistance

https://www.sciencedirect.com/science/article/abs/pii/S0955286307000848?via%3Dihub 1/3
19.09.2022, 19:52 Sulforaphane and erucin increase MRP1 and MRP2 in human carcinoma cell lines - ScienceDirect

Recommended articles

Cited by (53)

The phytoprotective agent sulforaphane prevents inflammatory degenerative diseases and age-
related pathologies via Nrf2-mediated hormesis
2021, Pharmacological Research

Show abstract

Molecular targets in cancer prevention by 4-(methylthio)butyl isothiocyanate - A

comprehensive review
2020, Life Sciences

Citation Excerpt :
…On the other hand, cell-cycle contains three checkpoints namely G1, intra-S and G2/M regulated through cell cycle
division16 (cdc) and CDKs [68]. There are many reports which show that 4-MTB-ITC exhibits biological activity similar
to SFN to induce apoptosis, cell-cycle regulation and growth inhibition in different types of cancers [69–77]. Many
reports indicate that SFN is converted to 4-MTB-ITC in humans [31].…

Show abstract

Consumption of baby kale increased cytochrome P450 1A2 (CYP1A2) activity and influenced
bilirubin metabolism in a randomized clinical trial
2020, Journal of Functional Foods

Show abstract

Modulation of hepatic ABC transporters by Eruca vesicaria intake: Potential diet-drug

interactions
2019, Food and Chemical Toxicology

Citation Excerpt :
…Exposure to Diplotaxis tenuifolia do not induce hepatic expression of P-gp, but expression of Mrp2 is decreased in
females and increased in males showing sex-related differences; regarding Bcrp, its expression is strongly induced in
both male and female mice. Concerning participation of glucosinolates, although being present in very low
concentrations in our accessions, it could not be discarded for E. vesicaria since sulforaphane and erucine induce P-gp
and Mrp2 in human hepatic carcinoma cells (Harris and Jeffery, 2008). The differential behavior of both species may lay
within variations in their phytochemical quantitative composition, since D. tenuifolia contains significantly higher
concentrations of total phenols and hydroxycinnamic acids (López Nigro et al., 2018 and Table 1 of this manuscript).…

Show abstract

The importance of ethnicity definitions and pharmacogenomics in ethnobridging and

pharmacovigilance
2018, Pharmacogenomics: Challenges and Opportunities in Therapeutic Implementation

https://www.sciencedirect.com/science/article/abs/pii/S0955286307000848?via%3Dihub 2/3
19.09.2022, 19:52 Sulforaphane and erucin increase MRP1 and MRP2 in human carcinoma cell lines - ScienceDirect

Show abstract

Modulation of expression and activity of intestinal multidrug resistance-associated protein 2

by xenobiotics
2016, Toxicology and Applied Pharmacology

Show abstract

View all citing articles on Scopus

View full text

Copyright © 2008 Elsevier Inc. All rights reserved.

Copyright © 2022 Elsevier B.V. or its licensors or contributors.

ScienceDirect® is a registered trademark of Elsevier B.V.

https://www.sciencedirect.com/science/article/abs/pii/S0955286307000848?via%3Dihub 3/3