John Paolo Bayro Lacanilao, a student in the Medtech 3-Y2-5 section, completed an activity using an electron microscope to view laboratory specimens. The document explains that a transmission electron microscope works by firing a beam of electrons through an extremely thin biological sample, and that the electrons' short wavelengths allow for higher magnification and resolution than with a traditional light microscope, enabling observation of cell structures at the nanoscale level.
John Paolo Bayro Lacanilao, a student in the Medtech 3-Y2-5 section, completed an activity using an electron microscope to view laboratory specimens. The document explains that a transmission electron microscope works by firing a beam of electrons through an extremely thin biological sample, and that the electrons' short wavelengths allow for higher magnification and resolution than with a traditional light microscope, enabling observation of cell structures at the nanoscale level.
John Paolo Bayro Lacanilao, a student in the Medtech 3-Y2-5 section, completed an activity using an electron microscope to view laboratory specimens. The document explains that a transmission electron microscope works by firing a beam of electrons through an extremely thin biological sample, and that the electrons' short wavelengths allow for higher magnification and resolution than with a traditional light microscope, enabling observation of cell structures at the nanoscale level.
Principle of Transmission Electron Microscope (TEM)
The mechanism of a light microscope is that an increase in resolution power decreases
the wavelength of the light, but in the TEM, when the electron illuminates the specimen, the resolution power increases increasing the wavelength of the electron transmission.
In a Transmission Electron Microscope, a beam of electrons is made to pass through the
specimen. The thickness of the specimen is maintained to 10nm. The beam after passing through the specimen is focused on a fluorescent screen, X-ray sheet etc to obtain a magnified image.