General Biology 2

Name: Aira Mae L. Albo Date: February 8, 2023

Section: STEM-A St. Minver Score:
Subject Teacher: Mrs. Marie Liz A. Cariaga, MPA, LPT

Learning Activity #1
Directions: Read the following questions. Answer the following questions

1. Describe the rDNA process?

• The rDNA or Recombinant DNA involves the process of combining the DNA of two
different organisms to produce a new genetic combination that are useful in serval
fields such as science, medicine, industry, and agriculture. The process introduces a
foreign fragment of DNA into the genome containing the desired gene. The desired
rDNA will be inserted into the host in various ways, such as biolistic, microinjection,
and many more.
2. Why is recombinant DNA important?
• Recombinant DNA, or rDNA, is important because it has been useful research tool for
scientists to study in laboratories. Also, with the use of the rDNA process, new products
that are helpful for humans are produced, such as vaccines that help prevent diseases.
3. How is recombinant DNA made?
• Recombinant DNA has been made possible using two types of enzymes, which are
restriction endonucleases and ligases. Scientists cut the original DNA and insert it with
ligase; the original DNA and the DNA with ligase will be pasted together, and they cut
them with restriction endonucleases.
4. What are the three different methods?
• There are three different methods of rDNA: (1) transformation, (2) non-bacterial
transformation, and (3) phage introduction. In transformation, the vector is inserted into
a host cell. While non-bacterial transformation is like transformation, the difference is
that this method does not use bacteria as a host. Lastly, in phage introduction, the
bacteriophages are viruses that infect and only replicate in bacterial cells.
5. What are the impacts of rDNA?
• There are many impacts that rDNA has had. However, like any other thing, there are
good and bad impacts. The good impacts are that it helps improve medicine and
livestock, it helps prevent genetic diseases, and it makes medicine and treatment safer
for pre-existing conditions. On the other hand, the bad impacts of it are that it can be
used to pick traits you want in the child, such as eye color, or it can raise environmental
concerns and experimental abuse by experts. Nevertheless, it gave us things that help
improve our lives, and they can be used to improve more in the future.