We used the NCBI database to identify the variant of interest to obtain the sequences'

geographical positions. After that, we used Clustal Omega, a multiple sequence alignment
program, to align all sequences. Additionally, I discovered the FASTA format, which allows
sequences to be written and put into the Clustal Omega toolbar. Following the execution of the
software, they asked us to identify distinct alterations within the alignment. In the alignment, the
asterisk indicates unmutated DNA, the dashed line indicates deletion, and I've also learned to
recognize substitutions. There is a phylogenetic tree with accession numbers in the Clustal
Omega database, which aided us in determining which mutation caused the outbreak in Guinea.