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  • Micro Spotting

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    Dhruv patel
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    The document discusses the contributions of Robert Koch including his discovery and cultivation of Treponema pallidum, the causative agent of syphilis. It also mentions his work developing germ theory and techniques like pasteurization and vaccines for cholera, anthrax and rabies. The document then provides information on various types of medical, infectious and biological waste and how they should be handled and disposed of. It concludes with descriptions of various microbiology laboratory equipment and methods like culture media, antibiotic susceptibility testing, and serological tests.

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    The document discusses the contributions of Robert Koch including his discovery and cultivation of Treponema pallidum, the causative agent of syphilis. It also mentions his work developing germ theory and techniques like pasteurization and vaccines for cholera, anthrax and rabies. The document then provides information on various types of medical, infectious and biological waste and how they should be handled and disposed of. It concludes with descriptions of various microbiology laboratory equipment and methods like culture media, antibiotic susceptibility testing, and serological tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    1 views59 pages

    Micro Spotting

    Uploaded by

    Dhruv patel
    The document discusses the contributions of Robert Koch including his discovery and cultivation of Treponema pallidum, the causative agent of syphilis. It also mentions his work developing germ theory and techniques like pasteurization and vaccines for cholera, anthrax and rabies. The document then provides information on various types of medical, infectious and biological waste and how they should be handled and disposed of. It concludes with descriptions of various microbiology laboratory equipment and methods like culture media, antibiotic susceptibility testing, and serological tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 59

    cultivation of treponema pallidum

    in testes of rabbit
    observed bacteria using single lense microscope constructed by him
    father of microbiology
    principal of fermentation
    pasteurisation
    vaccine development against cholera, anthrax,rabies
    liquid media concept
    germ theory
    causative agent-lesion

    constantly ass. with lesion


    isolate the organism
    inoculate into animal
    re-isolate
    Ab present in pt's serum
    diptheria toxin demonstration
    leptospirosis inoculation
    leprosy
    cultivation ,
    vaccine and
    treatment
    PPE kit

    donning- wear-- gown→mask→goggles→gloves

    doffing- removing -- gloves→gown→goggles→mask


    Anatomical waste
    Biological waste,Blood
    infections non plastic waste bags,blood/ body fluids
    Chemical solid & liquid waste
    Discarded linen waste
    Expired drugs
    soiled waste
    fixed needle with syringes
    syringes without needle
    infectious plastic
    urine bag , catheter
    IV set
    vaccutainer
    gloves
    plastic apron
    vials
    glassware ampoule
    slide
    broken and discarded glass
    body and dental implants
    biohezard symbol

    black bag
    disinfectant
    yellow bag
    dark than nutrient agar

    use:disc diffusion

    Muller Hinton agar

    autoclave
    s.typhi forms jet black
    colonies with metallic sheen
    due to H2S

    selective media
    simple medium - for growth of ordinary bacteria
    basal medium- for preparation of enriched medium
    enriched medium - lysed blood
    growth of hemophilus spp. bacteria
    enriched medium - growth of pneumococci

    differential medium- with type of hemolysis


    selective - enterobacteriace
    differential - LF&NLF
    horizontal

    CSSD
    Large lab.
    BMW

    vertical

    small lab.
    selective-cholera
    sucrose fermanting and non fermanting
    differential - in urine LF&NLF
    candle jar

    for growth of
    capnophilic
    bacteria
    oxygen removed by
    chemical reaction

    sod. bicarbonate and


    sod. borohydrate
    react in presence of
    h2o and produce H2
    and co2
    McIntosh and
    Filde's
    anaerobic jar

    manual method for


    anaerobic culture
    method
    hookworm
    infective form-
    filariform larvae L3
    wuchereria bancrofti
    brugia malayi
    - e.coli
    shigella

    salmonella
    + klebsiella
    pneumonia
    proteus
    helicobacter
    +. red ring
    e. coli

    - salmonella
    klebsiella
    - e. coli

    + klebsiella
    embryo

    polar bodies

    egg shell
    ingestion of eggs
    contaminated with
    finger, vegetable,
    fruits
    anthracis
    peptone water

    selective/basal media-
    peptone+ NaCl+ water
    striated embryophore
    AST: To determine which antibiotic
    will inhibit the growth microbes

    helps to test the infection

    antibiotic
    disc
    inoculation loop

    made-up of platinum
    inoculation of bac. on culture medium
    disposable swab

    sample collection from throat, cervix


    for preparation of law culture in AST
    syringe
    needle
    syringe
    petridish

    preparation of
    culture medium
    disc diffusion test
    latex
    agglutination precipitation
    test reaction
    precipitation reaction

    VDRL/RPR- for diag. of syphilis


    microtitre plate- ELISA
    96 well
    HIV tridot -
    immunochromatographic test

    cassette format

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