NON-PRINT LEARNING MATERIAL FOR LROW

Grade 11/12 General Biology 2

INTRODUCTION
Quarter and Week Number: Quarter 3 Week 1 B
Activity Title: “Recombinant DNA at Work”
MELCs: Discuss the applications of recombinant DNA. ( STEM_BIO11/12-IIIa-b-7)
Learning Targets: Value the importance of applications of recombinant DNA.
Content Developer:
Content Creator: Irrah Sheene D. Bucong
Illustrator/s:
Evaluator/s:

Pre-Test
Direction: The definition of the term in Column B is in Column A. Choose the entry in Column B that most accurately sums
up its definition in Column A. (Note: Format in Matching Form where students will just have to drag a line and point it to
their answer.)
Column A Column B
1. Level where gene products are manipulated in genetic engineering. a. Amino Acid
2. An organism that contains genes from other organisms is called _______. b. Bacillus thuringiensis
3. Microorganisms used in the application of recombinant DNA. c. Transgenic
4. Bacteria used in plants and it is poisonous to certain pests. d. DNA
5. A nonpathogenic bacterium that has the ability to produce proteins rapidly e. Bacteria
which is advantageous in developing biotherapeutics and vaccines.
f. Fungi
g. RNA
h. Pseudomonas fluorescens

ENGAGEMENT
Review (GUESS THE GIBBERISH)
Direction:
Part I. From your earlier discussion, what are the processes involved in genetic engineering?
1. EEL LECT TRUE FOUR A SHOWN
2. FRUIT OH PLUS FEW SHOWN
3. MIKE OH INN JAKE SHOWN
4. PART ICK KALE GONE
5. TRANCE DUCK SHOWN

Part II. Try to reorder the scrambled letters to give you a hint about the lesson that will be cover.

A I S T A N C P O L P I
Answer: _____ ______ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____
Lesson Presentation
Activity 1. One Pick Two Words
Guess the Answer (Note: Format is Guess the Answer in Lumi)
Direction: Try to determine which recombinant DNA applications best demonstrate the photos shown below.

T B N O R C / E S T P S E N I S R T E C A

M U H A N O R G H T W O M R H N E O

D F M I O Y O F D O U N T I R O I T L A N
M R I O E P V E T M N

Applications of Recombinant DNA

AGRICULTURE
 Transgenic plants, or plants that contain genes from other organisms, are now an important part in the field of
agriculture. By using recombinant DNA technology, plants can now be grown with genes responsible for producing
natural
insecticides. This technology reduces the need for synthetic or artificial insecticides and pesticides. In the Philippines,
recent developments have enabled plants to express a recombinant form of protein used by the Bacillus thuringiensis
bacterium. Recall the genetically modified maize known as Bt corn. This crop is widely used in organic farming because it
is poisonous to certain pests. This is greatly beneficial to farmers because Bt corn eliminates corn borers, which cause an
enormous amount of damage to corn crops in our country.

The following are some bacteria used in recombinant DNA technology:

1. Pseudomonas syriangae The recombinant variant of this bacterium is called the ice-
minus bacterium, which lacks the gene responsible for ice
formation. The iceminus bacteria prevent frost crystals from
forming in plants.
2. Pseudomonas fluorescens This is a nonpathogenic bacterium that has the ability to
produce proteins rapidly. This characteristic is
advantageous in developing biotherapeutics and vaccines.
3. Agrobacterium tumefaciens In its natural state, this bacterium has a tumorinducing (Ti)
plasmid that causes crown gall disease in plants. The said
Ti plasmid in the bacterium can be removed and replaced
with a recombinant plasmid. This enables the now-modified
bacterium to introduce beneficial genes to plants.
Other applications of recombinant DNA to agriculture are the following:
a. Crop Improvement
b. Genetically Engineered Traits: The Big Six
1. Herbicide Resistance
2. Insect Resistance
3. Virus Resistance
4. Altered Oil Content
5. Delayed Fruit Ripening
6. Pollen Control
c. Biotech Revolution: Cold and Drought Tolerance
and Weather- Guard Genes

MEDICINE
An important contribution of recombinant DNA technology in the field of medicine is the use of bacteria to create
substances that our body needs whether to maintain good health or to treat a disease. For example, human insulin is
 created using recombinant DNA to help diabetics. Also, the modified human growth hormone is widely used to support
the development of people who have a malfunctioning pituitary gland. This is of great benefit because it avoids the
practice of getting growth hormones from dead bodies, which can pose serious health risks. Other important substances
such as blood clotting factors (for people suffering from hemophilia) and hepatitis B virus surface antigens (for people
suffering from hepatitis B) are also products of recombinant DNA technology. Certainly, this technology has given us
many benefits in the field of medicine.

Other medical applications of recombinant DNA technology:

1. Production of proteins for replacement therapy (e.g. insulin), disease prevention (e.g. vaccines) and diagnostic
tests (e.g. monoclonal antibodies).
2. Treatment of genetic diseases (gene therapy).

FOOD INDUSTRY
Recombinant technology has also improved the food industry. Some of the crops that we eat are now resistant to
pests, diseases, and environmental stress. As a result, crop yields have increased and production costs have been kept
lowered.
Recombinant crops are also beneficial because of their improved nutritional quality and longer shelf life. Recombinant
technology can also be used for processing high quality fermented foods such as cheeses, pickles, wines, and beers.
Food products have been developed with genetic engineering. Cattle can be engineered to be bigger with higher
reproduction rates of better quality e.g. developing animals whose meat has less fat. It is possible to modify certain foods,
such as fruits and livestock, in terms of sweetness, color, and even nutritional value e.g. soybean, tomato and corn.

APPLICATION (Use H5P Find the Hotspot)

Directions: Identify what is being asked by clicking the correct picture/hotspot.
 1. Enzyme that allows fireflies to glow

luciferase photociferase lightociferase

2. Genetically modified maize

hemophilia homophilia hepaphilia

3. The recombinant variant of this bacterium is called the ice-minus bacterium

Agrobacterium
Pseudomonas fluorescens Pseudomonas syriangae
tumefaciens

4. This is a nonpathogenic bacterium that has the ability to produce proteins rapidly

Pseudomonas syriangae Agrobacterium

Pseudomonas fluorescens
tumefaciens

5. This bacterium has a tumorinducing (Ti) plasmid that causes crown gall disease in plants

Pseudomonas fluorescens Pseudomonas syriangae Agrobacterium

tumefaciens

CONSOLIDATION (Use H5P Essay, Limit to 200 characters)

Directions: Briefly discuss the importance of recombinant DNA’s applications.

YOUR ANSWER HERE

EVALUATION
Direction: The definition of the term in Column B is in Column A. Choose the entry in Column B that most accurately sums
up its definition in Column A. (Note: Format in Matching Form where students will just have to drag a line and point it to
their answer.)
Column A Column B
1. Level where gene products are manipulated in genetic engineering. a. Amino Acid
2. An organism that contains genes from other organisms is called _______. b. Bacillus thuringiensis
3. Microorganisms used in the application of recombinant DNA. c. Transgenic
4. Bacteria used in plants and it is poisonous to certain pests. d. DNA
5. A nonpathogenic bacterium that has the ability to produce proteins rapidly e. Bacteria
which is advantageous in developing biotherapeutics and vaccines.
f. Fungi
g. RNA
h. Pseudomonas fluorescens

Answer Key:
Pre-Test/Evaluation
1. D
2. C
3. E
4. B
5. H

Engagement
Part I.
1. ELECTROPORATION
2. PROTOPLAST FUSION
3. MICROINJECTION
4. PARTICLE GUN
5. TRANSDUCTION

Part II. APPLICATIONS

Lesson Presentation
Activity 2
Picture 1: Bt Corn/Pest Resistance
Picture 2: Human Growth Hormone
Picture 3: Modify Food
Picture 4: Nutritional Improvement

Application
1. luciferase
2. hemophilia
3. Pseudomonas syriangae
4. Pseudomonas fluorescens
5. Agrobacterium tumefaciens

REFERENCE/S: (Chicago Format)

Javier, Mark Archei O. Diwa Senior High School Series: General Biology 2. 4/F SEDCCO 1 Bldg. 120 Thailand corner
Legazpi
Streets, Legaspi Village, 1229 Makati City: DIWA LEARNING SYSTEMS INC, 2017.