Professional Documents
Culture Documents
2419-Article Text-6061-1-2-20220905
2419-Article Text-6061-1-2-20220905
Abstract
In this study, the manufacture of vegetable pesticides using natural ingredients of papaya leaf
extract and pandan leaf extract has been carried out as a substitute for synthetic pesticides to kill plant
pest organisms (OPT). This research is an experimental study using 250 experimental organisms Larvae
of Spodoptera litura which were divided into 5 groups. Each group consisted of 10 larvae of Spodoptera
litura. Each group was repeated 5 times. As test materials, papaya leaf extract and pandan leaf were
added to the media solution containing the test organisms. The concentration of each extract was different
for each treatment in each vial, for P1 (Papaya Leaf) which was 150 ml, P2 (Pandan Leaf) 150 ml, and
P3 300 ml (Mixture of Papaya Leaves and Pandan Leaves), and Control was 0ml. Data were obtained by
counting the number of larvae that died on the first day after application. Based on the data, the
percentage value of larval mortality was calculated using the Percentage formula, while the percentage
value of larval mortality in the control treatment was calculated using the Abbott formula, the average
value of mortality for each replication was calculated using the Datum formula. Based on the testing of
vegetable pesticides from papaya leaf extract and pandan leaf extract, the characteristics of vegetable
pesticide products from papaya leaf extract and pandan leaf extract were obtained, with the highest
mortality percentage obtained in P3 treatment (a mixture of papaya leaf extract and pandan leaf extract)
of 88%, while the percentage of mortality was 88%. in treatment P1 (pawpaw leaves) by 70%, and the
percentage value of death in treatment P2 (pandan leaves) by 38%. From these results, papaya leaves and
pandan leaves are effectively used as sources of environmentally friendly vegetable pesticides in killing
plant pests of Spodoptera litura larvae.
.
1
First Author name Running Article Title……………………………………..
pests (Rofinah Mbah Role, 2019) and replace extracts of water, ethanol and water:ethanol from
them with insecticides derived from nature such fragrant pandan leaves contain positive alkaloids,
as vegetable insecticides sourced from plants and tannins, flavonoids, and polyphenols. The
plants to reduce environmental damage (Saipur maximum total flavonoid and total phenolic
et al. , 2020). content in pandan leaf extract was obtained at
Armyworm (Spodoptera litura) is an 96% ethanol extract, respectively 478.7629 and
important pest that must be controlled because it 99.4086 mg/g (Agustiningsih et al., 2010).
can harm farmers and affect the quality of crop Therefore, a study was conducted using papaya
yields (Silalahi, Adi Sanjaya Hasian, Dedi leaves and pandan leaves as candidates for plant
Supriyatdi, 2021). This caterpillar attacks the pest control (IPM) and further testing was carried
leaves of the host plant. Characteristics of the out on the effectiveness of vegetable insecticides
damage caused by this pest is the perforated against armyworms (Spodoptera litura).
plant leaves. The young larvae damage the leaves
by leaving remnants of the upper epidermis and Methods
leaf bones, while the advanced larvae damage the
leaf bones and even attack the pods (Gu et al., Materials and Methods
2015; Hou et al., 2021). The clustering nature is Directional Methode
due to the way the moths lay their eggs in This research was carried out at the Chemical
groups. Yield losses due to armyworm attacks Laboratory of FKIP UNTAD and the Laboratory
can reach 80% if left unchecked (Mohamed et of Pests and Plant Diseases, Faculty of
al., 2019). For this reason, it is necessary to Agriculture, Tadulako University.
control the armyworm (Spodoptera litura) plant
pest by using insecticides from plants. Tools and Materials
The use of plant extracts as a source of The materials used in this study were papaya
vegetable insecticides because in plants there is a leaves, pandan leaves, methanol, water, S. litura
defense mechanism (Yudiawati & Hapis, 2016). larvae as experimental organisms. While the
One of the vegetable plants that can be used as tools used are dough mixing pan, blender, mixing
Plant Pest Control (IPM) is papaya leaves. spoon, test tube (vial), lighting lamp, filter,
Papaya leaves (Carica papaya L.) contain toxic measuring cup, funnel, digital balance and scales,
compounds such as saponins, karpain alkaloids, as well as a loupe/magnifying glass and
papain, flavonoids and are harmless to humans microscope as a tool for observing whether
and animals. Papaya leaf pesticides have high organisms die or not. observed test.
effectiveness and have a specific impact on pest
organisms (Jujuaningsih et al., 2021). Papaya General Procedure
plants contain a lot of papaya enzymes in their Making Vegetable Pesticides
leaves, namely proteolytic enzymes that play a
role in breaking down connective tissue, and Making papaya leaf methanol extract (Carica
when papaya enzymes enter the body of insects it papaya L)
causes chemical reactions in the body's Papaya leaves that have been cleaned
metabolism which can cause inhibition of growth weighed as much as 200 grams. Then mashed
hormones (Prihatini & Dewi, 2021). Papaya using a blender that has previously been added as
plants contain cysteine proteinase, alkaloids, much as 1 liter of water. Furthermore, 50 mL of
papaya enzymes, chymopapain, papaya latex methanol was added to the extract and allowed to
extract, saponins, flavonoids, karpanes and stand for 24 hours and filtered to obtain a
tannins. The mechanism of action of several suspension.
substances in papaya as anthelmintics is to break Preparation of pandan leaf methanol extract
down connective tissue, hydrolyze exoskeletal (Pandannus Amarylifoulius roxb)
proteins to damage the cuticle and expel the Clean pandan leaves weighed as much as
contents of the body, suppress the central 200 grams. Then mashed using a blender that has
nervous system of worms, and cause muscle previously been added as much as 1 liter of
paralysis in worms (Oktofani et al., 2019). water. Furthermore, 50 mL of methanol was
Another plant that can repel insects is added to the extract and allowed to stand for 24
pandan leaves. Another plant that can repel hours and filtered to obtain a suspension.
insects is pandan leaves. The results of
phytochemical tests on the content of chemical Production of Combined Extracts (Papaya Leaf
compounds in pandan leaves have been reported Extract and Pandan Leaf Extract)
by Prameswari and Widjanarko (2014) that
2
Volume, 6, No. 1, 2017, pp-pp Jurnal Akademika
Kimia
Special Procedure
∑ xi
i=1
Pest or test organism dyeing method n
The method of immersing the pests or n
test organisms is to dip the test pests in the form Where is : ∑ xi = Total of all Datum
of caterpillars into a solution of vegetable i=1
50
45
40
35
30
25
20
Vegetable pesticide treatment group
15
10
5
0
Kelompok Perlakuan Pestisida Nabati
4
Volume, 6, No. 1, 2017, pp-pp Jurnal Akademika
Kimia
90
80
Percentage of mortality
70
60
50
40
Vegetable pesticide
30 treatment group
20
10
0
Kelompok Perlakuan Pestisida
Nabati
6
Volume, 6, No. 1, 2017, pp-pp Jurnal Akademika
Kimia
Nani Heryani, & Rejekiningrum, P. (2019). Silalahi, Adi Sanjaya Hasian, Dedi Supriyatdi, A.
Jurnal Sumberdaya Lahan Volume 13 S. (2021). Respons Ulatgrayak (Spodoptera
Nomor 1 , Juli 2019. Sumber Daya Lahan, litura) Terhadap Lama Perendaman
13(2), 63–71. Tembakau Rajang (Nicotiana tabacum L.)
Sebagai Insektisida Nabati. Agrosains :
Nguyen, T. T., Rosello, C., Bélanger, R., & Ratti, Jurnal Penelitian Agronomi, 23(2), 83.
C. (2020). Fate of residual pesticides in
Fruit and Vegetable Waste (FVW) Subiyakto, 2005. Pestisida nabati dan
processing. Foods, 9(10). pemanfaatannya. Yogyakarta
Oktofani, L. A., Suwandi, J. F., Kedokteran, F., Tampubolon, K., Sihombing, F. N., Purba, Z.,
Lampung, U., Parasitologi, B., Kedokteran, Samosir, S. T. S., & Karim, S. (2018).
F., & Lampung, U. (2019). Potensi Potensi metabolit sekunder gulma sebagai
Tanaman Pepaya ( Carica papaya ) sebagai pestisida nabati di Indonesia. Kultivasi,
First Author name Running Article Title……………………………………..