Professional Documents
Culture Documents
Simultaneous Measurement of Ultrasonic Velocity and Sample Thickness and Its Application To NDE of Composites
Simultaneous Measurement of Ultrasonic Velocity and Sample Thickness and Its Application To NDE of Composites
Citation: The Journal of the Acoustical Society of America 90, 2358 (1991); doi: 10.1121/1.402135
View online: https://doi.org/10.1121/1.402135
View Table of Contents: https://asa.scitation.org/toc/jas/90/4
Published by the Acoustical Society of America
Simultaneous ultrasonic velocity and sample thickness measurement and application in composites
The Journal of the Acoustical Society of America 92, 669 (1992); https://doi.org/10.1121/1.405279
to do the matrix inversion.The SVD methodcoupledwith the finite- 11:00
elementmethod implementationof HIE enabledthe extensionof the
8PAb9. The effects of ultrasound on membrane-bound ATPase
near-field acoustical holography technique from two- to three-
dimensionalsources.Test resultsare given. activity. Mumtaz A. Dinno (Dept. of Physics,Univ. of Mississippi,
University, MS 38677), Robert J. Fisher, John C. Matthews,
Lawrence A. Crum, and Wendy Kennedy (Univ. of Mississippi,
University, MS 38677)
10:15
Ultrasoundfrequencies rangingfrom 26 kHz-10 MHz are usedin a
8PAb6. Reconstruction of transducer surface velocity distributions numberof applicationsvaryingfrom cell disruptionsto therapeuticand
from a limited number of acoustic pressurefield measurements.J. R. diagnosticprocedures.Previousresultsfrom our laboratoryhave dem-
Blakey (Dept. of Physics,Univ. of Surrey, Guildford, Surrey GU2 onstratedthat 1-MHz ultrasoundat therapeuticintensitiescausessig-
5XH, U.K.) nificantchangesin the electrophysiological propertiesof epithelialtis-
sues.The natureof thesealterationsindicatedthat, amongothereffects,
The accuratepredictionof the acousticfieldof a transducerrequires
the Na/K-dependent ATPase activity of the tissuewasreduced.In this
a detailedknowledgeof its surfacevelocitydistribution.The normal
study,the effectsof ultrasonicirradiation (30 kHz) on enzymeactivities
surfacevelocitydistributionof pistonlikeplane transducerscan be de-
in two modelsystemshavebeenexamined:onemembraneincorporated
ducedfrom a suitablespatialtransformationof the acousticpressure
(Na/K-dependent ATPase in inverted erythrocyte ghostsfrom rat
distributionmeasuredover a planeparallelto the transducersurface.
blood) and the other in "free" solution (ATP-dependent firefly
This methodnormally requiresa largenumberof time-consuming pre-
luciferin/luciferasereaction). Ultrasonicirradiationproducedirrevers-
cision measurementsand a correspondingcomputationaleffort. Less
ible decreases in the activityof the enzymein eachsystem.At present,
intensemethodsof transducercharacterization,which can predict ra-
the analysisleadsone to concludethat sono-chemical(includingfree
diation fieldsto an acceptableaccuracy,are thereforeof someinterest.
radical) and not thermaleffectson the enzymemoleculesare responsi-
The "effectiveradius" method is a well-known exampleof such an
ble for the activitydecreases. Theseresultssupportthe hypothesis that
approach.This contributiondiscusses a methodof reconstructingap-
the effectsof ultrasonicirradiation were, in part, due to effectson the
proximatesurfacevelocitydistributionsfrom the inversionof the axial
activity of Na/K-dependentATPase. Furthermore,the resultsof this
pressuredistributionand presentssometypical resultsobtainedfrom
studydemonstratethat sono-chemical-induced enzymeactivitychanges
this approach.
may representa muchmore commonand pervasiveeffectthan is com-
monlyrealized.[Work supportedby the NIH underGrant No. 5-RO1-
CA-39374-05.]
10:30
2358 J. Acoust.Soc. Am., Vol. 90, No. 4, Pt. 2, October 1991 . 122nd Meeting:AcousticalSocietyof America 2358