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KENZA MAX BiochemisTry

Auto Analyser for Routine Biochemistry Tests <<<<<<

KENZA MAX Biochemistry

SERVICE MANUAL
V 2008/09

BIOLABO
Biolabo Service Manual

© 2002 BIOLABO S.A. All Rights Reserved.


The information contained in this manual is subject to change without notice at
any time. The collection and verification of all documentation has been carried
out with due care; however, BIOLABO is not responsible for the utilization of
this documentation.

BIOLABO S.A.
Les hautes Rives
02160 MAIZY-FRANCE

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Biolabo Service Manual

TABLE OF CONTENTS

1. INTRODUCTION ............................................................................................................................5
1.1 INSTRUMENT’S DEVICES DESCRIPTION ...................................................................7
1.2 KENZA MAX BIOCHEMISTRY - GENERAL DESCRIPTION and Block Diagram .........10
1.3 How to change the voltage according to the country ......................................................13
1.4 Power supply and mother board description ...................................................................13
2.TECHNICAL CHARACTERISTICS .................................................................................................14
3.TEST AND UTILITY PROGRAMS ..................................................................................................15
Printer Interface Test ................................................................................................17
ABS Mode.................................................................................................................18
Pump Calibration program ........................................................................................18
Host programming: ...................................................................................................21
Hardware Test ..........................................................................................................21
Setup program ..........................................................................................................23
4. REPLACEMENTS AND ADJUSTAMENT......................................................................................24
BATTERY REPLACEMENT .....................................................................................24
LAMP REPLACEMENT AND CENTERING .............................................................25
DARK CURRENT SETTING.....................................................................................27
FILTER CHECK ........................................................................................................28
PREAMPLIFIER BOARD GAIN ADJUSTMENT ......................................................29
FILTERS REPLACEMENT .......................................................................................31
CPU BOARD ADJUSTMENT ...................................................................................34
PERISTALTIC PUMP TUBE REPLACEMENT/MANTEINANCE .............................35
ADJUSTMENT OF TEMPERATURE MEASUREMENT ..........................................37
CHANGING HEATING ELEMENTS .........................................................................39
5. INSTRUMENT REPROGRAMMING ..............................................................................................41
REPROGRAMMING THE MASTER.........................................................................41
REPROGRAMMING METHODICS ..........................................................................41
REPROGRAMMING HEADER AND METHOD .......................................................41
6. LIST OF SPARE PARTS................................................................................................................43
APPENDIX 1: motherboard hardware CHECKPOINTS........................................................45
APPENDIX 2: TROUBLESHOOTING ...................................................................................49
APPENDIX 3: REDUCING CARRY-OVER AND WORKING VOLUME USING AIR-
GAP .......................................................................................................................................51
APPENDIX 4: READING IN STANDARD CUVETTE WITH LESS THAN 1500 L .............53
APPENDIX 5: HOW TO LOCK METHODS...........................................................................55

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Biolabo Service Manual

Release History

Release Date Modifications and Upgrades


_1 09/01/2006 Inserted description for command “6” in hidden commands
section of hardware test section
_2 11/01/2006 Inserted Appendix 5. Corrected explanations of key “p” and
“P” in Hardwre test section

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Biolabo Service Manual

1. INTRODUCTION

The KENZA MAX Biochemistry is an interferential filter photometer, completely


managed by microprocessors. It performs photometric measurements and processes
them according to programs with parameters that can be entered by the operator. It
executes, in a rapid and precise manner, most of important chemistry and
hematology tests. Particularly, the following determinations can be carried out:

ABSORBANCE

END-POINT

KINETICS

FIXED-TIME

MULTISTANDARD

The instrument is equipped with a two-way flow cell system; it ensures low carry-over
values even with limited sample volumes. Disposable macro and micro cuvettes
(glass or plastic), with an optical path of 1 cm, can be used by simply removing the
flow cell from the reading compartment and placing it on the right-side one. Seven
filters (and one additional empty position) are included in the instrument. The
selection of the interferential filter is automatic, with powered handling managed by a
microprocessor. This feature makes reading easier and eliminates filter selection
errors that may occur in instruments which have a manual selection.
The optical part is very sophisticated: it consists of a high-power halogen lamp (20
W) whose light beam is centered by a quartz lens, thus allowing a high accuracy in
measurements even when reduced-volume cuvettes are used.
A 10-position dry incubator which can contain both square and cylindrical cuvettes
allow the sample incubation before reading. The temperature of the incubator is
equal to the one of the reading cell and is selectable from 20°C to 40 °C.
The execution of the analyses and instrument programming are simple and
performed by means of a keyboard, following the instructions shown on the display.
This display also shows the status and error/fault messages. The analytical results
are directly displayed in the measuring units selected by the current program. The
language of instructions can be selected between English and other customizable
languages. The instrument is provided with a 24-columns thermal printer that can
print analytical results as well as program parameters (multistandard and kinetics
results are also available in a graphic plot). All printed information is sent to a serial
RS-232 standard output. The printer can be also completely disconnected as well as
can be disabled the graphic plot.
An advanced software guides and controls all operations carried out by the user. An
acoustic signal further helps the user, by emitting a sound of a different tone from the
usual one in case you pushed a wrong key.

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A hundred and twenty programs can be stored. To carry out an analysis, it is


important to enter all the parameters correctly, including the K and standard values
where necessary.
The instrument is supplied already programmed. Anyway, it is advisable to check that
the entered parameter values correspond to those stated in the methods.
A modification of the analytical parameters can be done by the operator before
carrying out the analysis.
The instrument has an internal memory that can store up to 400 result. Tests are
automatically stored in memory every time they are executed. Results can be
recalled in every moment in the right section of the program manager. In this way the
instrument can printout result as a batch or a profile analyzer.
The instrument is provided with two level QC program, avalaible for 30 indipendent
tests.
All KENZA MAX Biochemistry are subjected to a double quality control, photometric
and clinical-chemical.

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1.1 INSTRUMENT’S DEVICES DESCRIPTION

Keyboard. The keyboard (Figure 1) is used in a functional way, for moving inside the
Menu. Data entering is guided by the emission of a beep when a key is pressed. The
keyboard consists of 8 keys, 4 of which are arrow, for moving inside of the Menu and
4 functional. Two keys (ENTER/YES and STOP/NO) are both functional for two kind
of operation. The following table shows which functions can be entered by pressing
each of the functional keys:

Keypad KEYS PS/2 Keyboard Function


MENU
Tab ↔ To enter inside the MENU

Enter ↵
ENTER/YES:
Store the entered values. Confirm.
STOP/NO Interruption of the operations in progress. (always active
Esc
key).
WASH Washing the flow cell. Connects the peristaltic pump
----- continuously. This key functions only when the instrument is
on the main menu.

⇒ Right movement arrow

⇐ Left movement arrow

⇓ Down movement arrow

⇑ Up movement arrow

Be careful in the case you open the instrument for operation because of
possible electric shocks

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Biolabo Service Manual

Figure 2: Instrument description

Display. It is graphic 240x128 pixel back-illuminated liquid crystal type, consisting of


two 16-character lines. Usually, the first line displays the main parameters of the
analysis in progress (analysis number, item and K). The second line guides the
operator in performing the analysis in an interactive way. Furthermore, the
instrumental status and error/fault messages are also displayed.

Printer . It is a 24-columns thermal type. It can be enabled or disconnected by the


operator and can print analytical results and parameters. In multistandard and kinetic
analysis it can also plot a graphic of the results. The operating voltage range for
driving the head and motor is from 23 V to 25 V. Under the printer there is the
interface board which contains the control circuit, his operating voltage range is
from 4.75 V to 5.25 V. This circuit perform a software and hardware control to detect
abnormal temperatures, position of the head-up (when head is in the up position the
circuit does not activate the heat element) and when paper runs out.

Incubator chamber. It consists of a single thermostated aluminum block with 9 +1


housings for cylindrical and prismatic cuvettes, arranged on three rows of three, plus
another position on the right side of the reading cell. Thermostating is obtained by
means of semiconductor devices which ensure an accurate regulation of the
temperature. The two housings, separated from the previous ones, are: a reading cell
compartment (on the left) and the another thermostated compartment where to place
the flow cell when another type of cuvette is used for reading. Peltier cell is an
element build with two different kind of metal soldered each other. In accordance with
the Peltier effect a current through these metals generate a difference of temperature
proportional to his intensity. So if one surface of the cell is cooling, the other surface
is heating. Other two housings, separated from the previous ones are: a reading cell
compartment (on the left) and an empty compartment which contain to place the flow
cell when another type of cuvette is used for reading.

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Between the reading compartment and the 10 housing for cuvettes there is the
temperature sensor IC, it is inserted in a whole fill with thermally-conductive epoxy to
ensure a good thermal contact.
The reading compartment is central between the lamp from a side and the filter
selector and preamplifier board on the other side.
In the side of the lamp there is a lens to agree the light in the optical path through the
reading compartment, from the other side there is a small glass to avoid that the dust
dirty the filter.

Filter selector and preamplifier board. Connected by a bracket with the incubator it
is constituted by the motor which makes the filter wheel turn around. On the wheel
there is also an Hall sensor for signaling of the filters position. Inside the same box
there is the preamplifier board where the two trimmers RV0 and RV1 are located,
RV0 is for the offset calibration and RV1 for adjustment of the amplification, on which
it is possible to act through the holes present on the right side of the bracket. (Refer
to chapter 4 for further details).

Aspiration spout. A teflon tube which protrudes from the front panel of the
instrument and intakes the sample into the flow cell.

Sample lever (Push-button). Situated under the aspiration spout. When pressed, it
turns on the peristaltic pump to intake the sample.

Flow cell. The instrument can read either in flow cell or in standard cuvette.
Instrument is equipped with 18 L flow cell. Pay attention to the polarity of the flow
cell when you insert it in the reading compartment. Be sure that the face with the
white arrow is towards the operator.
The inclination of the flow cell inside the reading compartment is optimal since it
ensures that no air bubbles are formed inside the cell itself.

CLEANING OF FLOW CELL:

To clean the cell inside, press WASH key aspirating sodium hypochlorite (10% diluited)
and air. In case this is not enough, you can use a deproteinzer such as Perchloric acid
(0.33 molar) in order to remove residual and incrostation.
A diluted solution of sodium hypochlorite (10 %) or a liquid detergent for glassware is
recommended to clean the flow cell outside.

WARNING Pay attention do not take off the flow cell by the tubes, you could break the cell
in the top.

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1.2 KENZA MAX BIOCHEMISTRY - GENERAL DESCRIPTION AND BLOCK


DIAGRAM

The instrument is connected to the main power supply (220 or 110, autosensing) by
a cable with two plugs, one side of the cable must be inserted into a main socket the
other side in the socket of the polysnap.
In the polysnap are located two fuses for each phase, with a switch for line and
neutral. The power supply is ten leaded to the autosensing switching power supply
for delivering the right tension in the connector J1 (refer to check points for the
tension values).
Note that the switching power supply needs the motherboard and a loads to work
properly (otherwise it will oscillates at the frequency of about 1 Hz).

The mother board of the KENZA MAX Biochemistry contains the circuits which carry
out most functions of the instrument, except the printer interface, that is handled by a
separate board.

The mother board supplies and controls all instrument’s devices and sensors.

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Figure 3 : Block diagram

Display 240x128 Pump motor

Power supply

Motherboard
RS-232 / PS-2

Optics:
Fans controlling
Filter motor
Lamp controller
Preamp board
Thermostate ctlr

Printer

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Biolabo Service Manual

Instrument back side:

In the back side of the instrument you can find the following item:

❷ RS-232 D-type 9-pin male connector for connection with an external serial printer
or PC through the serial port. Refer to the Appendix C of this manual for the serial
transmission protocol used by the instrument to output results. You can use
BIOLABO software to connect the instrument to a PC: ask your dealer for further
details.

❷ PS2 connector to connect a keyboard to KENZA MAX Biochemistry

❷ Serial number of the instrument

❷ ON/OFF switch to turn ON and OFF the instrument.

❷ Cooling fan.

❷ Fuse holder, containing number 2 fuses. Refer to technical specification


paragraph for fuses values.

• Waste connector: attach here the waste tank pipe.

Figure 4: View of the back side of the instrument

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1.3 HOW TO CHANGE THE VOLTAGE ACCORDING TO THE COUNTRY

The instrument is equipped with an auto sensing power supply; there is NO NEED
OF REGULATION between 110V and 220V, 50 and 60 Hz independently.

1.4 POWER SUPPLY AND MOTHER BOARD DESCRIPTION

The master motherboard can operate with two 24K bytes EEPROM chips, allowing
QC data, result memory and methods setting to be stored inside.

Temperature calibration is achivied by software.


Display contrast and other calibration are achivied by software.
In the motherboard there are only 2 trimmers that are required for “Out of range” and
“Log calibration”, named RV1 and RV2 and are needed to eliminate the offset of the
operational amplifiers, this means that the value of the measure of the light is shifted
by this regulation.
This trimmer are generally set in the factory and never needs to be chanaged or
moved, because they are only related to the motherboard, and not to the preamplifier
board or system performance and regulations.

ASTEC LQ153 Motherboard


Power Supply

Block Diagram of the Power Supply area

In the incubator there are 2 Peltier cells, driven in parallel for thermoregulation by the
microprocessor. The Peltier cells work like a heater or cooler in accordance with the
direction of the current, so they are connected through a H-Bridge
(Q11,Q12,Q15,Q16) that can change the direction of the current that passes through
the Peltier in accordance with the MCU control signal.
There are 2 LEDs in the mother board for a visual signaling, if the voltage across the
Peltier cells is in positive or in negative ( when the cells are working as coolers the
green LED (LD4) is on, when they are working as heaters the red LED (LD5) comes
on).
When both the LED are off, this means no voltage is present in the Peltier cells.

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2.TECHNICAL CHARACTERISTICS

Photometric system:
Light source: long life halogen lamp 20 W
Spectral field: 340 nm - 690 nm
Filter wheel: automatic by motor
Filters: 340, 380, 405, 505, 546, 578, 630, plus one empty
position; pass-band 6 nm
Detector: at solid state

Thermostatic control:
Heating element: refrigerating and heating Peltier cell
Temperature: 20 to 40 °C
Temperature precision: + 0,1 °C
Stabilizing time: 10 minutes
Thermostatic block: at 10 positions per square or round test tubes, macro
and semimicro

Flow system:
Flow cell: 80 µL two ways
Typical working volume: 500 µL
Minimum working volume: 350 µL
Carry over: lower than 1%
Aspiration: peristaltic pump with programmable sipping volume

Measuring system:
Reset: automatic
Measuring range: -0.200 +2.500 OD
Photometric linearity: + 1 % from 0 to 2.000 OD
Photometric accuracy: + 1 % from 0 to 2.000 OD
Precision: + 1 digit
Drift: lower than 0.005 O. D. per hour

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Biolabo Service Manual

Reagent volume in cuvette: 1 ml (minimum) for macro cuvette, 0.3 ml for


semimicro cuvette
Reagent volume in flow cell: 0.35 ml (minimum)
Wash function: manual
Measuring method: End-Point, Kinetic, Fixed-Time, Differentials,
Multistandard.

Programming and data display:


Keyboard: 8 multifuncional keys; external connection for PS-2
keyboard
Display : graphic 240x128 pixel back-illuminated
Thermal printer: built-in graphic printer with 24 columns
Continuous memory: 120 programs
Serial output: RS-232 standard

Physical characteristics:
Power supply: 220 V - 110 V, 50/60Hz
Capacity: 160 VA
Weight: 11 Kg
Dimensions: H. 35 cm - W. 34 cm - D. 24 cm

Serial transmission protocol:


The serial output, standard RS-232, uses the following transmission protocol:
38400, N, 8, 1.

Serial connection:
The output connector, standard 9 poles, is located on the rear of the instrument. The
connections are the following:
pin 2: input data.
pin 3 output data.
pin 5 reference signal.
The connection with serial input of a personal computer type I.B.M. or compatible
can be effected by a cable with direct connection of the three pins above described.

3.TEST AND UTILITY PROGRAMS

To verify the correct functioning of all mechanical and electrical parts, the instrument
has some utility programs.

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Biolabo Service Manual

They can be selected interacting with user-friendly software of the instruments. The
following picture shows the basic layout of the display:
Date

Software Version
V 1-x.xx 18/10/04 12.35.56 Time

Temperature Window
Top Window:
shows the status of
the machine

Body Window:
shows results and the
parameter actually Menu window
selected

Error messages area

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Biolabo Service Manual

Printer Interface Test


To carry out this test, switch on the instrument while pressing the paper feed button
(LF). The electronics, that controls the printer, performs a test cycle printing a
complete series of characters. Please note that in this case only the printer interface
electronics is involved.
If the LF button is pressed only for few seconds, only text printing is performed.
If LF is kept pressed during the test, also the default logo (if present) will be printed to
test the graphic mode of the software.

The main service menu appears like this window (English language),
To enter the Service Menu, press MENU when you are in Main Menu, then select
“SERVICE” using DOWN arrow and press ENTER. The Body Window appears as
follows:

ABS Mode

Pump Calibration

Diagnostic & Service

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Biolabo Service Manual

ABS Mode

To select ABS Mode, enter Service Menu as explained above.


The analyzer will ask to you, in sequence, to select the wavelength of the filter, the
volume of the sample to be aspirated and the temperature of the incubator. These
settings can be carried out using arrows’ buttons.
The following picture shows the Body Windows for the wavelength setting, for the
other two features the Body Window will appear similar:
Read filter (nm)

340

Use arrows to select the filter, then press ENTER to confirm and go to the next
setting (Temperature and aspiration volume).
On the contrary press STOP to exit from ABS mode. When you completed the
settings, on the Body Window will appear the message “INSERT BLANK”. Insert the
blank cuvette into the reading cell and press ENTER. Then will appear the message
“INSERT SAMPLE”: insert the sample cuvette into the reading cell and press
ENTER. The absorbance value will continuously be shown until STOP key is
pressed. This method, applied to sample control solutions with known absorbance
value at a specific wavelength, can be used to verify the instrument accuracy.

Pump Calibration program

The peristaltic pump is moved by a stepper motor which permit to intake the volume
of sample with high level of precision.

WARNING This calibration should be carry out periodically, because variation in the
elasticity of peristaltic silicon tube could vary the quantity actually sipped.

5 mL

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Biolabo Service Manual

Procedure:
- Select the Pump calibration program.
- Put the aspiration spout into a cuvette with 5 ml accurate of distilled water.
- Press the push button. The peristaltic will begin to work.
- When the target quantity has been aspirated (5 mL) press immediately the push
button. The LCD display the value in ms of the time that occurred. This value is
stored and is used as reference for the instrument to intake any quantity of
sample. Optimal value goes from 8000 to 14000.
- The instrument will return automatically to main menu after few seconds.

Value:
less than 8000 abnormal motor running. Too fast or wrong tube.
8000- 14000 the rubber is efficent and in good condition.
Bigger than 14000 Obstruction on the aspiration spout or tube consumed.

Diagnostic & Service

You can reach “Diagnostic & Service” selecting “Diagnost. & Service” in the Service
Menu. The Body Window will appear as follows:

Quick Diagnostic

Service Only

If you select “QUICK DIAGNOSTIC” the analyser will print some self-diagnostic
parameters, which colud be helpful when you call service to solve any problem.
This section will explain the meaning of the parameters printed by “Quick diagnostic”
mode.

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Biolabo Service Manual

FILTER #1: 2577 Transmittance value of the filter 1 OK


FILTER #2: 1794 Transmittance value of the filter 2 OK
FILTER #3: 965 Transmittance value of the filter 3 Too low! Energy to HIGH. Needs gain
Adjustement or coating

FILTER #4: 3127 Transmittance value of the filter 4 OK


FILTER #5: 15500 Transmittance value of the filter 5 Too high!! Energy too low! Needs replace
Or coating removal

FILTER #6: 1550 Transmittance value of the filter 6 OK


FILTER #7: 1886 Transmittance value of the filter 7 OK
FILTER #8: 222 Transmittance value of the filter 8 (Empty)

CAL: 10740 Internal calibration. Range: 8000-16000


PMP: 9000 Pump calibration Volume. Range: 6000-13000
Tw: 23.2 C Flow cell and incubator temperature
Ta: 21.4 C Motherboard temperature
Fan: 30 C The fan starts working at this temperature

Lamp On = 090 s Lamp’s warming up time


Lamp Off = 006 s Delay turning off time whne lamp is idle
MotL: 1000 ms Stepping motor parameter (Should be always this value)
MotV: 2000 ms Stepping motor parameter (Should be always this value)
NLet: 04 System parameter (Should be always this value)
NMet: 120 System parameter (Should be always this value)
Timeout: 60000 System parameter (Should be always this value)
OnCond: 03750 System parameter (Should be always this value)
Amax: 00450 Maximum of absorbance value for KIN and FXT mode
IntChk: 002 System parameter (Should be always this value)
Int ok: 012 System parameter (Should be always this value)
CODelay: 003 System parameter (Should be always this value)
m = 0.049505 Temperature parameter (different for each instrument)
q = 0.049509 Temperature parameter (different for each instrument)

EEP: 3kEv_1.04 Version of EEPROM program


HDWR: 1 Hardware version
MSG: 1.05 Language messages version
SWR: 1.09 Master firmware version
SLV: 1.05 Slave firmware version

[0]: 0, 0 Linearity compensation


[0]: 9362, 9362 Linearity compensation
[0]: 18724, 18724 Linearity compensation
[0]: 28086, 28086 Linearity compensation
[0]: 37449, 37449 Linearity compensation
[0]: 46811, 46811 Linearity compensation
[0]: 65535, 65535 Linearity compensation

Service only

This area is reserved to service engineers.

Host programming

Hardware Test

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Host programming:

Host programming is useful for transferring data to the instrument and setting up the
method inside the 120 test memory (this is useful for customizing most of the method
parameters, like K-Factor, test name, standard concentration, number of decimal
point and so on).
You can use this program also to restore factory settings (in case of memory
corruption). You need a special program (called HM3000.exe), running in Microsoft
Windows (c), to transfer the method file and parameters to the instrument.
Refer to chapter 5 on how to use this program to connect it with the instrument.

This program is also used to upgrade the Slave MCU (in case it is needed), by the
mean of a special program (refer to BIOLABO website or contact BIOLABO service
engineer for further info).

This program is not suitable for upgrading the Master MCU (you MUST use, in this
case, the Bootloader, so you have to start with the instrument OFF).

Hardware Test

NOTE:
In order to work with this program you have to connect EXTERNAL PS-2 keyboard.
Otherwise you will never access to the basic hardware function of the instrument.

Be aware that matrix keyboard do not produce any command (except double click
when STOP is pressed) and WASH (to verify instrument washing), in order to make it
possible to diagnostic it.
Only PS2 code is allowed in this program.

Check of matrix keyboard:


To check if the matrix keyboard is working properly you can press the key of the
matrix keyboard and compare the value displayed with the corresponding one on the
table.

ENTER MENU STOP WASH


10 1 10000 100K 10M 1M Exit 1K
K = 1’000
M = 1'000’000

To check if the PS2 keyboard is working it is enough to press NUM LOCK key and verify the
corresponding LED status (ON and OFF).

NOTE:
PS2 key are KEY sensitive. So it MAKES different from command capital and small

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Biolabo Service Manual

Other useful key in service program:

KEY FUNCTION
b Buzzer test
T Temperature calibration (Warning: special device is needed!!) DO NOT PERFORM WITHOUT
SPECIAL TOOLS
R Execute automatic and continuous reading on the selected filter
1-8 Makes filter selection:
1 = 340
2 = 405
3 = 380
4 = 505
5 = 546
6 = 578
7 = 630
8 = Empty
H Execute filter wheel homing and realignment
p Printer test
0 Test Serial port to PC
V Burn in mode for instrument (term cycle) (TEST PROGRAM)
L Set linearity compensation values. (Warning: special device is needed!!) DO NOT PERFORM
WITHOUT SPECIAL TOOLS
C Execute instrument calibration
D Test/Set RTC (Real Time Clock): date and time
f Test ON/OFF fan
v test ON /OFF lamp
B Execute automatic bichromatic filter cross check. (TEST PROGRAM)

c Make it possible to execute visual lamp alignment (Low emission mode)


P Enable or disenable Method lock (see Appendix 5)

NOTE (hidden commands):


There is some special program that should never be entered unless it is specified by
BIOLABO service engineer or it is known what you are doing.

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INCORRECT OPERATION MAY DAMAGE INSTRUMENT PERMANENTLY

Hidden command program.


You enter in this modality by pressing Q key.
On the screen it is not shown anything, in order to prevent accidental intrusion.
Possible commands:

1= Modify ‘AMAX’.
2= Restore of the EEPROM and HEADER factory setting (in case of corruption of memory)
3= Temperature offset adjustment (q).
NOTE: To decrease temperature, increase the offset, to increase the temperature, decrease
the offset.
Refer to chapter 4, section ADJUSMENT OF TEMPERATURE MESUREMENT

4 = Restore default language


5 = Enable temperature in status bar.
6 = Edit wavelength for optional filter
9 = Enable/disable BURN IN (DO NOT USE !!!) THE INSTRUMENT WILL STOP TO RESPOND IF
BURN IN IS ENABLED.
If accidentally activate, deactivate it by following the same procedure.

Setup program

In this program it is possible to set the some print characteristic, the language
regarding the display message and to active the air gap choosing the quantity of air
and the delay time between the sample aspiration and air aspiration, and so on.

The air gap could be introduced to save a small quantity of sample, actually to
reduce the carry-over effect it must be aspirated a volume of sample enough to fill
the flow cell and all the way from the aspiration spout, so is possible to intake the first
part with the sample to fill the flow cell and following part with air to fill the tube.

WARNING Pay attention to set the quantity of air not too big otherwise the air enter
in the flow cell.
This will lead to incorrect result and reproducibility problems.

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4. REPLACEMENTS AND ADJUSTAMENT

About the electronic there are 3 board that could be replaced.


Interface printer board No adjustment is required.
Mother board This board is supplied calibrated but needed to be checked the
temperature control because each temperature sensor is slightly different.
Preamplifier board This replacement requires the calibration of the absorbance
with a cuvette of know value adjusting the trimmer R1. The trimmer R4 is for adjust
the amplifier to set the value A in the range 2500 - 7500 for all filter.
After this replacement the calibration for Hematocrit and Erythrocytes tests could be
no correct, if you want verify these test you can ask B.S.I. headquarters instructions.

PRELIMINARY PROCEDURE

Before performing the following operations, make sure that the instrument is
switched off, open the instrument unscrewing the four screws from the bottom that fix
the cover on the basis and place the cover on the right side without turning it over in
order to be able to operate on the keyboard.

BATTERY REPLACEMENT

You need to replace the battery when you have a malfunctioning on date and time.
The nominal voltage value at battery pins cannot be less of 3V.

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LAMP REPLACEMENT AND CENTERING

1. Perform the preliminary procedure


2. Disconnect the lamp cables from connector J4 (refer to Appendix 1)
unscrewing both screws
3. Replace the lamp just unscrewing the two screws on lamp support. Pay
attention not to touch with fingers the lamp bulb. Connect the lamp cables to
connector J4
4. After the change, you only need to center the lamp with the reading aperture
of the photometer, which is inside the reading hole. The following pictures
shows how to perform the centering

Reading
aperture of the
photometer

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Biolabo Service Manual

5. It is possible that after having centered the lamp, there is too much energy on
the preamplifier board. You need thus to perform PREAMPLIFIER BOARD GAIN
ADJUSTMENT (see the related section in this chapter).

Pag 26 of 55
Biolabo Service Manual

DARK CURRENT SETTING

The preamp board has a bias current due to the offset of its operational amplifier.
This current has to be compensated in order to have correct results.
First of all, perform preliminary procedure to open the instrument. Then connect a
multi meter to test points TP2 and TP5 on the mother board (see Appendix 1). These
test points are directly connected to preamp output: you have to connect the red tip
of the multi meter to TP2 (OFFSET value) and the black tip of the multi meter to TP5
(GND).
Then switch on the instrument and insert the cuvette into the reading hole. Rotate the
cuvette for 90°, in order to have the dark face of the cuvette in front of the
photometer. Select the best resolution you have on your multimeter (you need at
least 0.1 mV for resolution). Act on trimmer RV0 of preamp board (see following
picture) until you reach a value between 0.5 mV and 1mV.

NORMAL ROTATED

Trimmer RV0: Offset

Pag 27 of 55
Biolabo Service Manual

FILTER CHECK

Perform “Quick Diagnostic” program as explained in chapter 3. The instrument


prints out a value that is reversely related to the transmittance values of the filters
(which is the output of the A/D converter): these values should stay in the range
1200-8000.
In fact, if this value is less than 1200, this means that the energy values related to
the filter is too high and the preamp board is working in saturation: the instrument
can only read high absorbance samples (which bring energy to lower values),
without accuracy or good resolution.
If the ADC output is more than 8000, this means that the energy value oh the filter
is too low and the preamp board is working near interdiction zone, so the
instrument can read correctly only samples with low absorbance and samples
with high absorbance will be underestimated.
In order to have all the values in the safe area, filters are coated externally with
some gelatins to make light attenuation (only 340 nm filter isn’t coated and all the
other filter are equalized to this value).
The following picture sums up the concept:

Offset subtracted to reading: can only read high


absorbance values without accuracy and resolution
(Preamp board in saturation)

1200

SAFE RANGE

8000

Underestimate high absorbance values


(Preamp board in interdiction zone: values with
low absorbance values are accurate and correct)

ADC
OUTPUT

Pag 28 of 55
Biolabo Service Manual

PREAMPLIFIER BOARD GAIN ADJUSTMENT

If filters’ transmittance values are not all in the safe area, you should adjust the gain
of the preamp board (ADC converter). Enter “Hardware Test” and press “R” in PS2
keyboard to execute a continuous reading of the transmittance values of the filters.
Press “1” in PS2 keyboard to select filter one (340 nm) and check the value if it is not

Trimmer RV1:
GAIN

in the safe area (1200-8000), you have to act on trimmer RV1 of preamplifier board
until the value is not in the correct range.

Repeat the operation for all the seven filters (you can select a filter pressing the
correspondent number on the PS2 keyboard).
If for one or more filter is not possible to reach Safe Area, you need to add or remove
the gelatin coating or to replace the filter (see next pictures as examples and next
paragraph for description).

1 2 3 4 5 6 7

1200
ALL in SAFE AREA

8000
340
630

ADC Output

Pag 29 of 55
Biolabo Service Manual

1 2 3 4 5 6 7

1200
Filter 3 is not in safe area:
remove some gelatin
coating to increase energy
value
8000
340
630

ADC Output

1 2 3 4 5 6 7

1200 Filter 3 is not in safe area, add


some gelatine coating to
decrease energy value
(gelatine will block more
8000
photons)
340 630

ADC Output

Pag 30 of 55
Biolabo Service Manual

FILTERS REPLACEMENT

If you need to replace some filter, first of all you have to perform preliminary
procedure.
Then unscrew the four screws which tie the two stirrups of dissipater block to the
basement.
Disconnect all the cable of the entire block from the motherboard and remove the
entire block (dissipater + incubator) from the instrument.
Then unscrew the four screws shown in the following picture to separate filter wheel
block from the incubator.

Then rotate filter wheel by hand, until the hole on the bracket coincides with the one
on the filter wheel.

Pag 31 of 55
Biolabo Service Manual

Use a 1,5 mm spanner to loosen the grain and liberate the filter wheel. Be sure that
the little washer is coupled with the stepping motor shaft.

Washer: indispensable for a


perfect coupling between
stepping motor and filter
wheel

The following picture shows the filter wheel:

Pag 32 of 55
Biolabo Service Manual

With a screwdriver remove the fixing washer and replace the filter paying attention
that the mirror face is turned downwards, furthermore pay attention to the gelatins
under the filter (for 340 nm filter no gelatin is present). AVOID touching the 2 optical
surfaces of the filter and the gelatins by hands not to soil them.
After the replacement, remount all the parts and perform preamplifier board gain
adjustment.

POS Filter (nm) Equalization

1 340 Nothing

2 405 1 gel of 2 + 1 gel of 5

3 380 1 gel of 1 + 1 gel of 5

4 505 1 gel of 4 + 1 gel of 1

5 546 1 gel of 4 + 1 gel of 2

6 578 1 gel of 4 + 1 gel of 3

7 630 2 gel of 3

8 free -

Equalization to be used as starting point for filter coating. May changes according with
filter transimattance, age, lamp emission photodetector spectral response.

Pag 33 of 55
Biolabo Service Manual

CPU BOARD ADJUSTMENT

The motherboard doesn’t need any hardware calibration, because they have been
carried out by the constructor. Please do not act anyway on trimmer RV1 and RV2 on
motherboard: these trimmers have been factory pre-setted and hardware calibration
during the motherboard quality control process.

Pag 34 of 55
Biolabo Service Manual

PERISTALTIC PUMP TUBE REPLACEMENT/MANTEINANCE

Perform the preliminary procedure, in order to access to the peristaltic pump tube.
You can replace peristaltic pump tube by simply detaching it from the plastic tip
connected to the TEFLON tube.
See the figure below for a schematic view of the pump:

PERISTALTIC PUMP

OUT
WARP DIRECTION

TEFLON

IN
PLATE

ADAPTER BETWEEN TEFLON


AND ELASTIC TUBE

To change the tube disconnect the elastic tube from its 2 adapters, by simply pulling
them out.
Then you can replace the tube and reconnect the 2 adapters.

ADAPTER BETWEEN TEFLON


AND ELASTIC TUBE

Pag 35 of 55
Biolabo Service Manual

Before closing the cover again, be sure that the peristaltic pump elastic tube has a
suitable strength. You can easily check for this by aspirating some distilled water and
see if anything drop form the intake tip outside the instrument. No drops must be
present.

PUS

No drops present!

If you have still some drops, despite having already changed the tube, you must
insert some plastic ring (shipped with the tube) in order to increase the strength of
the elastic tube.

Usually inserting one on the top and one on the bottom it is enough for adding the
missed power to the peristaltic pump. Refer to the figure below for the correct way of
placing this 2 rings:

Insert here the PLASTIC RINGS for increasing the


strength of the peristaltic pump.

Usually placing 2 rings (1 on top + 1 on bottom) it is enough to make the peristaltic


pump working properly. If not so, add more 2 rings.

Remember that if you don't have the need to add this ring, don't add them to the
tube, because they increase the strength, reducing the lifetime of the peristaltic pump
tube.

NOTE: You must recalibrate the peristaltic pump aspiration volume after having
changed on of the part above.

Pag 36 of 55
Biolabo Service Manual

ADJUSTMENT OF TEMPERATURE MEASUREMENT

To perform this operation you don’t need to open the instrument, so you don ‘t have
to perform preliminary procedure: the operation will be carried out via software. Insert
a thermocouple thermometer into reading cell and swicth it on. Next picture shows
the thermocouple thermometer.

CAP 37.0

THERMOCOUPLE

CUVETTE

1000 uL WATER
THERMOMETER

NOTE: Be sure that your thermocouple thermometer is calibrated and with 0.1 °C
of accuracy in measurements. If you set temperature at a wrong value Kinetic
analysis results will be mistaken.

NOTE: If you use mercury thermometer you should insert it in a cuvette filled with
water NOT DIRECTLY IN THE INCUBATOR. You need 0.1 °C of accuracy in
measurements. You need more time to reach a stable temperature with a mercury
thermometer, since it affects the measurement greater than a thermocouple (typically
the waiting time are doubled, i.e. 1h.)

Pag 37 of 55
Biolabo Service Manual

Connect a PS-2 keyboard to the instrument and switch it on. Enter “Service Only”
Menu as explained in chapter 3 and select “Hardware Test”. Press “Q” key on PS-2
keyboard to enter hidden command program. The display will show only the script
“Press ENTER to return!”. Don’t press ENTER but press “3” key to perform
temperature offset adjustment. Press ENTER as the instrument ask you to confirm.

On the display will appear this window:

Increase or
Decrease?

Yes=Inc. NO=Dec

You have to press “YES” or “ENTER” to increase the offset value or “NO” or “ESC” to
decrease it.

Increasing offset will decrease the temperature value, decreasing offset will
increase temperature value.

Depending on your choice, the display will show one of this window:

- 0.00 + 0.00

With arrows and number keys, choose the value to increase or decrease offset value
and press ENTER. The display will show the script “>>>>> OK! <<<<<”: press
ENTER again.
Wait until the temperature value on your thermocouple thermometer become stable.
Repeat the operation until the temperature value reachs 37 °C. Then exit from
“Hardware Test” and press ENTER as the instrument ask if you want to save the
changes.

Pag 38 of 55
Biolabo Service Manual

CHANGING HEATING ELEMENTS

Heating elements (Peltier Cells) are located under the incubator and fixed to the heat
dissipator in two proper lodging.
Perform the preliminary procedure to open the instrument. Then extract dissipator-
incubator block as explained in “Filters Replacement” section.
Now unscrew the four black screw which tie incubator to the dissipator. The following
picture shows two of these screw, on the opposite side you will find the others.

Then pull up the incubator: next picture will show the two blocks separated (in this
picture also the lamp has been removed)
Thermal
conductive
PAD

Peltier
Cell

Peltier
Cell

Replace Peltier cells according to the picture above. Remount incubator on the
dissipator: you can remove one of the two lateral stirrup to perform the operation
easier. Be sure to screw the four black screw of the incubator so that pulling peltier
cables doesn’t make the cell to move.
Pag 39 of 55
Biolabo Service Manual

The Peltier are coupled to the incubator and the heatsinker with a thermic conductive
PAD. This PAD ensure good heating transmission and increase heating and cooling
efficency.
Assemble the Peltier cells in this way:

Incubator

Pink thermic
conductive
PELTIER CELL PAD

Heatsinker Grey thermic


conductive
PAD

Important:
Avoid thermal short circuit to increase heating efficiency. In case there is not heating
or cooling efficiency a parasitic thermal path may be present.

GOOD NO GOOD

Pag 40 of 55
Biolabo Service Manual

5. INSTRUMENT REPROGRAMMING

This section will explain how you can reprogram the instrument

REPROGRAMMING THE MASTER

It is possible to reprogram the software of the instrument reprogramming the flash


memory of Master microprocessor. For further details contact BIOLABO :

Web Site: www.biolabo.fr


E-mail: info@biolabo.fr

REPROGRAMMING METHODICS

It is possible to reprogram all the methods via PC, if you need to personalize analysis
parameters or if you need to restart with default parameters.
For further details contact BIOLABO:

Web Site: www.biolabo.fr


E-mail: info@biolabo.fr

REPROGRAMMING HEADER AND METHOD

With Header Programmer software it is possible to modify via PC the name of the
instrument printed when it is turned on and to personalize the report printed with test
results in “Result Printout” operation. You can reach “Result Printout” from “Print”
Menu.
Connect the instrument to the PC through a serial cable (RS-232). Switch on the
instrument and enter “Host Programming” as explained in chapter 3.
Run Header Programmer from your PC: on your display will appear the software’s
window as follows:

Pag 41 of 55
Biolabo Service Manual

KENZA MAX Biochemistry

First of all, select the correct COM Port.


Then write the new name for the instrument (if you want to change it): 24 characters
are available.
Click “Update!” button in the Header section of the window: the counter box will count
the number of byte transferred. Wait until the transmission is complete (the counter
will stand still).
To personalize the report, first of all select how many rows should it have, clicking on
the arrows buttons. Then edit the boxes you have enabled: for each row you have 24
characters available.
When you are ready, click “Update!” button in Report section of the window, wait until
the transmission is complete.
Then click “Exit” button and exit from “Host Programming” menu in the instrument.
Then switch off the instrument.
For further details contact BIOLABO :

Web Site: www.biolabo.fr


E-mail: info@biolabo.fr

Pag 42 of 55
Biolabo Service Manual

6. LIST OF SPARE PARTS

For spare parts list, visit section “SPARE PARTS LIST” in our website www.biolabo.fr
and follow the link spare parts.

Pag 43 of 55
Biolabo Service Manual

Pag 44 of 55
Biolabo Service Manual

APPENDIX 1: MOTHERBOARD HARDWARE CHECKPOINTS

TP2 J16

TP
5

J5

J6

J9

J11

J15

J8 J3

J17
J2

J12
J18

J10 J14

J7
J4

J20 Pag 45 of 55
J1
Biolabo Service Manual

This section will show all voltage values and checkpoints for KENZA MAX
Biochemistry motherboard:

J1: POWER SUPPLY

Pin 1: +15 V
Pin 2 : GND
Pin 3: -15 V
Pin 4: +5V
Pin 5: GND
Pin 6: NC
Pin 7: +21
Pin 8: GND

J2: External FAN (FAN 1)

Pin 1: +12V to +14 V


Pin 2: GND

J3: Incubator FAN (FAN 2)

Pin 1: +12V to 14V


Pin 2: GND

J4: LAMP

Pin 1: 10V
Pin 2: GND

J5: PHOTOMETER (PREAMP)

Pin 1: +12V
Pin 2: GND
Pin 3: -12V
Pin 4: 0V to 10V

J6: TEMPERATURE

Pin 1: +12V
Pin 2: GND
Pin 3: NC
Pin 4: Temperature (°C) = Vout (mV)/10

J7: DISPLAY BACK ILLUMINATION

Pin 1: 3V to 5V
Pin 2: GND

Pag 46 of 55
Biolabo Service Manual

J8: DISPLAY PINS

Pin 1: GND
Pin 2: +5V
Pin 3: NC
Pin 4 – Pin 16: LOGIC SIGNALS
Pin 17- Pin 18: NC
Pin 19:LOGIC SIGNAL
Pin 20-Pin 21: NC
Pin 22: -17V to –10V (Contrast setting voltage)
Pin 23: GND
Pin 24: NC

J9: LED

Connector absent

J10: PUSH BUTTON

Pin 1: +5V normally, 0V when pressed


Pin 2: GND

J11: BUZZER

Pin 1: +5V
Pin 2: +5V normally, 0V when buzzer is on

J12: SERIAL PORT

Pin 1: NC
Pin 2: Host_TX (Standard RS-232 voltage values)
Pin 3: Host_RX (Standard RS-232 voltage values)
Pin 4: GND Float

J13: PRINTER

Pin 1: +5V
Pin 2: +5V
Pin 3: PRN_TX (Standard RS232 voltage values)
Pin 4: PRN_RX (Standard RS232 voltage values)

J14: PS-2 KEYBOARD (External)

Pin 1: +5V
Pin 2: +5V
Pin 3: GND
Pin 4: Standard PS-2 voltage values
Pag 47 of 55
Biolabo Service Manual

Pin 5: Standard PS-2 voltage values


Pin 6: GND

J15: KEYBOARD

Pin 1: 0V (button pushed) 5V (button not pushed)


Pin 2: 0V (button pushed) 5V (button not pushed)
Pin 3: 0V (button pushed) 5V (button not pushed)
Pin 4: 0V (button pushed) 5V (button not pushed)
Pin 5: 0V (button pushed) 5V (button not pushed)
Pin 6: 0V (button pushed) 5V (button not pushed)

J16: Peristaltic Pump Motor

Pin 1: +12V
Pin 2: +12V / -12V

J17: FILTER WHEEL MOTOR

Pin 1: 0V / 5V
Pin 2: 0V / 5V
Pin 3: 0V / 5V
Pin 4: 0V / 5V

J18: HOME SENSOR FILTER WHEEL

Pin 1: +5V
Pin 2: 0V / +5V
Pin 3: GND FLOAT
Pin 4: NC

J19: PELTIER

Pin 1: 0V / +5V
Pin 2: 0V / +5V
Pin 3: 0V / +5V
Pin 4: 0V / +5V

J20: PELTIER POWER SUPPLY

Pin 1: +5V
Pin 2: GND

TP2: PREAMP OFFSET TEST POINT

TP5: PREAMP GROUND TEST POINT

Pag 48 of 55
Biolabo Service Manual

APPENDIX 2: TROUBLESHOOTING

This appendix shows the error messages related to issues that the user can normally
solve by himself: if the problem persists, or a problem not listed below arise, contact
your dealer.
Excluding the main plug fuses, the instrument has no user serviceable parts:
only trained technicians are allowed to service the instrument. An
unauthorized action on the instrument may invalidate its safety and features,
beside void the warranty.
In case of suspect maflunctioning of the instrument, we recommend to check the
instrument with coloured solution or control serum of known value.
In the following table we describe messages and flags which appear on the Body
Window or in a window which appears in the middle of the display:

MESSAGE DESCRIPTION POSSIBLE OPERATIONS

ERROR -1 The sample absorbance is too high or the Press ENTER to repeat the reading otherwise
reading is impossible, due to faulty instrument dilute the sample. If message persists call our
service technicians.
Printer error Error when printing Press STOP to disconnect the printer, or any
other key to try again.

Temperature The thermostat is already regulated at the


Window is fixed required temperature.

Temperature The thermostat has not yet reached the required Wait.
Window is temperature.
blinking
Temperature The temperature is disenabled
Window shows
----
Const ERROR Critical error in EEPROM Call service.

EEP0 ERROR Critical error in EEPROM Call service


EEP1 ERROR Critical error in EEPROM Call service
EEP2 ERROR Critical error in EEPROM Call service
Delay XXX XXXX = time (in seconds) required to complete Wait or press STOP to interrupt the analysis
the analysis.

--- In a KIN or FXT analysis, the absorbance- Dilute the sample.


variation of the sample is too high during the first
time-interval (Delay).

LLLL In an MSD analysis, the sample concentration is The sample concentration is lower than the
less than that of the lowest standard. minimum range of the method.

HHHH In an MSD analysis, the sample concentration is Dilute the sample.


greater than that of the highest standard

CALIBRATE It appears when you select an EP analysis with Press ENTER if a new calibration is required.
(Y/N) standard or FXT with standard. It allows the The instrument will be ready to read the
possibility to select a new calibration with the standard: INSERT STANDARD. Press STOP if
standard or read directly the sample cuvette a new calibration is not necessary. The
instrument is ready to read the sample:
INSERT SAMPLE.

Pag 49 of 55
Biolabo Service Manual

D The analytical result exceeds the linearity limit of Dilute the sample
the reagents.
* The thermostat has not reached the right Repeat the analysis when the thermostat and
temperature when the analysis was performed the reagents have reached the right
(blinking temperature window). The result temperature.
therefore, is not correct.
L The analytical result is less than the lower limit
value of the method.
H The analytical result is more than the higher limit
value of the method.

In the following table, we describe Error Messages which appear in the Error Message Area

MESSAGE DESCRIPTION POSSIBLE OPERATION


TA ERROR Error reading internal temperature Call service
TW ERROR Error reading incubator temperature Call service
PRN PAPER The roll of printing paper is over Insert a new roll of printing paper
PRINT KO The printer is disabled or Disable printer or call service
malfuncioning
PRN TEMP Overheating of LPT Wait
OVERHEATING Instrument overheating. Turn off and wait few minutes
H.FIL KO Malfunctioning of filter positioning Call service
system

The instrument is provided with 2 fans. One fan is automatically controlled by


microprocessor and it is switched on and off as required, according to the internal
temperature of the instrument. Internal fan is always on and cool the incubation
group.

Pag 50 of 55
Biolabo Service Manual

APPENDIX 3: REDUCING CARRY-OVER AND WORKING VOLUME USING AIR-


GAP

Carry-over is the residual solution inside the flow cell that affects the final result of a
photometric measurement. This quantity depends by several factors, but the most
important for our purpose are:
• The density of the solution used
• The absorbance value of the solution
• The internal cleanliness of the flow cell
• The quantity of aspirated volume (sample volume)
• The flow cell volume. The instrument can work either with 80 uL flow cell (that is
the standard flow cell) or with the 18 uL flow cell (upon request).
The instrument is shipped with its carry-over value indicated in the documentation
that refers to its internal Quality Control. Instrument carry-over must be less than 1%
to pass the Quality Control.

To reduce instrument carry-over several measures can be taken:


• Use 18 uL flow cell instead of 80 uL flow cell.
• Wash the instrument with sodium hypoclorite to clean internally the flow cell.
• Use a greater quantity of reading solution.
• Use air-gap.

Air-gap can reduce dramatically carry-over effects, but requires a little of experience
in working with it. Air-gap is an instrument features that allows the User to set up a
Sample volume, a Delay time and an Air anspiration volume. Basically the instrument
performs a DOUBLE ASPIRATION (the first for the sample, the second for the air).
In fact, after each sample the peristaltic pump is turned OFF for a programmable
amount of time (User should remove the reading solution from the aspiration
spout during this delay) and is turned ON again for a certain amount of time in
order to aspirated a programmable volume of air.

This parameters are General Setup Parameters (not test parameters) so will remain
the same for all the test.
DELAY = 00 s
ANSP. VOL. = 00000 uL
The instrument is shipped with this 2 parameters disabled (their values are set to
0000).

Recommended value for beginner are:


DELAY = 03 s
ANSP. VOL. = 00150 uL
In this way the instrument (when a sample in flow cell is executed during a test)
makes a double aspiration described in these 3 steps:
1. Aspirate the sample volume programmed for that specified test.
2. Wait for the programmed Delay time
3. Aspirate the programmed quantity of air.

Pag 51 of 55
Biolabo Service Manual

You just have to enter “Setting” in “Setup” Menu to set this 2 parameters (They are
called “Air aspiration” and “Delay”).

According to the test you are executing and to the test manufacturer you can change
these parameters in order to fit their best values.

Pag 52 of 55
Biolabo Service Manual

APPENDIX 4: READING IN STANDARD CUVETTE WITH LESS THAN 1500 L

The analyzer is shipped with the reading height that exactly matches the reading
aperture in the flow cell (the same height for 18 or 80 uL flow cell). In this condition
the analyzer can work correctly in the flow cell and with a minimum reading volume of
1500 uL in standard plastic cuvette (10 mm optical path), like the ones supplied with
the instrument.
Some kits however have a reading volume of less than 1500 uL (for example 1000
uL). With this kit is no possible to work with the instrument unless:
1) Use reduce volume cuvette (still 10 mm optical path). Contact your nearest
distributor for more information.
2) Adjust the PVC screw in the incubator chamber in order to increase the reading
height. In this way you can reduce the volume according to your needs, but
remember that the light beam does not match anymore the flow cell reading
aperture and you have to setup it again if you want to work again with flow cell.

The figure below explains how to adjust the reading height (regulating the height of
the PVC screw) in order to work with flow cell or reduced volume. Refer to the
following table for more information:

PVC Screw HEIGHTFLOW CELL REDUCE VOLUME CUVETTE STANDARD CUVETTE


(h)
0* -Figure A - OK OK (Minimum Volume 1500
uL)
2.5 turn * -Figure B- NO OK OK

PVC SCREW

*: Note that the screw height is measured according to the turn from the down
position.

Pag 53 of 55
Biolabo Service Manual

NOTE:
Use maximum care when performing this operation, since an excessive strength may
damage permanently the PVC screw inside the reading incubator chamber.

Pag 54 of 55
Biolabo Service Manual

APPENDIX 5: HOW TO LOCK METHODS

This procedure allows to lock Methods in KENZA MAX Biochemistry.


Be sure that a PS2 keyboard is connected to the instrument.
In Main Menu, press MENU on the little keyboard on the instrument (or TAB on PS2
Keyboard) and select “Service” using arrows, then press ENTER.
V 1-x.xx 18/10/04 12.35.56

MAKE A SELECTION 37°C

001- OPEN -EP Setup


002- OPEN -KIN
003- OPEN -EP Print
004- OPEN -EP
005- OPEN -KIN Service

In the SERVICE menu, select “Diagnostic & Service” and press ENTER.

ABS Mode

Pump Calibration

Diagnostic & Service

Than select “Service Only” and press ENTER. Finally select “Hardware Test” and
press ENTER.
Press “P” in the PS2 keyboard (be sure to press “P” because “p” would start printer
test), the instrument ask you if you want to lock methods: press ENTER to confirm.
The display will show four digit to insert a password:

____

Write your password using PS2 keyboard and press ENTER.


Press ESC to exit from “Hardware Test”: the instrument will ask you if you want to
save changes: press ENTER to save.
Press ESC several times to return Main menu.

Following the same procedure and inserting the correct password will allow you to
unlock Methods.

Pag 55 of 55

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