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Manuel Tech Kenza Max - Service 17 11 2008
Manuel Tech Kenza Max - Service 17 11 2008
SERVICE MANUAL
V 2008/09
BIOLABO
Biolabo Service Manual
BIOLABO S.A.
Les hautes Rives
02160 MAIZY-FRANCE
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TABLE OF CONTENTS
1. INTRODUCTION ............................................................................................................................5
1.1 INSTRUMENT’S DEVICES DESCRIPTION ...................................................................7
1.2 KENZA MAX BIOCHEMISTRY - GENERAL DESCRIPTION and Block Diagram .........10
1.3 How to change the voltage according to the country ......................................................13
1.4 Power supply and mother board description ...................................................................13
2.TECHNICAL CHARACTERISTICS .................................................................................................14
3.TEST AND UTILITY PROGRAMS ..................................................................................................15
Printer Interface Test ................................................................................................17
ABS Mode.................................................................................................................18
Pump Calibration program ........................................................................................18
Host programming: ...................................................................................................21
Hardware Test ..........................................................................................................21
Setup program ..........................................................................................................23
4. REPLACEMENTS AND ADJUSTAMENT......................................................................................24
BATTERY REPLACEMENT .....................................................................................24
LAMP REPLACEMENT AND CENTERING .............................................................25
DARK CURRENT SETTING.....................................................................................27
FILTER CHECK ........................................................................................................28
PREAMPLIFIER BOARD GAIN ADJUSTMENT ......................................................29
FILTERS REPLACEMENT .......................................................................................31
CPU BOARD ADJUSTMENT ...................................................................................34
PERISTALTIC PUMP TUBE REPLACEMENT/MANTEINANCE .............................35
ADJUSTMENT OF TEMPERATURE MEASUREMENT ..........................................37
CHANGING HEATING ELEMENTS .........................................................................39
5. INSTRUMENT REPROGRAMMING ..............................................................................................41
REPROGRAMMING THE MASTER.........................................................................41
REPROGRAMMING METHODICS ..........................................................................41
REPROGRAMMING HEADER AND METHOD .......................................................41
6. LIST OF SPARE PARTS................................................................................................................43
APPENDIX 1: motherboard hardware CHECKPOINTS........................................................45
APPENDIX 2: TROUBLESHOOTING ...................................................................................49
APPENDIX 3: REDUCING CARRY-OVER AND WORKING VOLUME USING AIR-
GAP .......................................................................................................................................51
APPENDIX 4: READING IN STANDARD CUVETTE WITH LESS THAN 1500 L .............53
APPENDIX 5: HOW TO LOCK METHODS...........................................................................55
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Release History
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1. INTRODUCTION
ABSORBANCE
END-POINT
KINETICS
FIXED-TIME
MULTISTANDARD
The instrument is equipped with a two-way flow cell system; it ensures low carry-over
values even with limited sample volumes. Disposable macro and micro cuvettes
(glass or plastic), with an optical path of 1 cm, can be used by simply removing the
flow cell from the reading compartment and placing it on the right-side one. Seven
filters (and one additional empty position) are included in the instrument. The
selection of the interferential filter is automatic, with powered handling managed by a
microprocessor. This feature makes reading easier and eliminates filter selection
errors that may occur in instruments which have a manual selection.
The optical part is very sophisticated: it consists of a high-power halogen lamp (20
W) whose light beam is centered by a quartz lens, thus allowing a high accuracy in
measurements even when reduced-volume cuvettes are used.
A 10-position dry incubator which can contain both square and cylindrical cuvettes
allow the sample incubation before reading. The temperature of the incubator is
equal to the one of the reading cell and is selectable from 20°C to 40 °C.
The execution of the analyses and instrument programming are simple and
performed by means of a keyboard, following the instructions shown on the display.
This display also shows the status and error/fault messages. The analytical results
are directly displayed in the measuring units selected by the current program. The
language of instructions can be selected between English and other customizable
languages. The instrument is provided with a 24-columns thermal printer that can
print analytical results as well as program parameters (multistandard and kinetics
results are also available in a graphic plot). All printed information is sent to a serial
RS-232 standard output. The printer can be also completely disconnected as well as
can be disabled the graphic plot.
An advanced software guides and controls all operations carried out by the user. An
acoustic signal further helps the user, by emitting a sound of a different tone from the
usual one in case you pushed a wrong key.
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Keyboard. The keyboard (Figure 1) is used in a functional way, for moving inside the
Menu. Data entering is guided by the emission of a beep when a key is pressed. The
keyboard consists of 8 keys, 4 of which are arrow, for moving inside of the Menu and
4 functional. Two keys (ENTER/YES and STOP/NO) are both functional for two kind
of operation. The following table shows which functions can be entered by pressing
each of the functional keys:
Enter ↵
ENTER/YES:
Store the entered values. Confirm.
STOP/NO Interruption of the operations in progress. (always active
Esc
key).
WASH Washing the flow cell. Connects the peristaltic pump
----- continuously. This key functions only when the instrument is
on the main menu.
⇑ Up movement arrow
Be careful in the case you open the instrument for operation because of
possible electric shocks
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Between the reading compartment and the 10 housing for cuvettes there is the
temperature sensor IC, it is inserted in a whole fill with thermally-conductive epoxy to
ensure a good thermal contact.
The reading compartment is central between the lamp from a side and the filter
selector and preamplifier board on the other side.
In the side of the lamp there is a lens to agree the light in the optical path through the
reading compartment, from the other side there is a small glass to avoid that the dust
dirty the filter.
Filter selector and preamplifier board. Connected by a bracket with the incubator it
is constituted by the motor which makes the filter wheel turn around. On the wheel
there is also an Hall sensor for signaling of the filters position. Inside the same box
there is the preamplifier board where the two trimmers RV0 and RV1 are located,
RV0 is for the offset calibration and RV1 for adjustment of the amplification, on which
it is possible to act through the holes present on the right side of the bracket. (Refer
to chapter 4 for further details).
Aspiration spout. A teflon tube which protrudes from the front panel of the
instrument and intakes the sample into the flow cell.
Sample lever (Push-button). Situated under the aspiration spout. When pressed, it
turns on the peristaltic pump to intake the sample.
Flow cell. The instrument can read either in flow cell or in standard cuvette.
Instrument is equipped with 18 L flow cell. Pay attention to the polarity of the flow
cell when you insert it in the reading compartment. Be sure that the face with the
white arrow is towards the operator.
The inclination of the flow cell inside the reading compartment is optimal since it
ensures that no air bubbles are formed inside the cell itself.
To clean the cell inside, press WASH key aspirating sodium hypochlorite (10% diluited)
and air. In case this is not enough, you can use a deproteinzer such as Perchloric acid
(0.33 molar) in order to remove residual and incrostation.
A diluted solution of sodium hypochlorite (10 %) or a liquid detergent for glassware is
recommended to clean the flow cell outside.
WARNING Pay attention do not take off the flow cell by the tubes, you could break the cell
in the top.
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The instrument is connected to the main power supply (220 or 110, autosensing) by
a cable with two plugs, one side of the cable must be inserted into a main socket the
other side in the socket of the polysnap.
In the polysnap are located two fuses for each phase, with a switch for line and
neutral. The power supply is ten leaded to the autosensing switching power supply
for delivering the right tension in the connector J1 (refer to check points for the
tension values).
Note that the switching power supply needs the motherboard and a loads to work
properly (otherwise it will oscillates at the frequency of about 1 Hz).
The mother board of the KENZA MAX Biochemistry contains the circuits which carry
out most functions of the instrument, except the printer interface, that is handled by a
separate board.
The mother board supplies and controls all instrument’s devices and sensors.
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Power supply
Motherboard
RS-232 / PS-2
Optics:
Fans controlling
Filter motor
Lamp controller
Preamp board
Thermostate ctlr
Printer
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In the back side of the instrument you can find the following item:
❷ RS-232 D-type 9-pin male connector for connection with an external serial printer
or PC through the serial port. Refer to the Appendix C of this manual for the serial
transmission protocol used by the instrument to output results. You can use
BIOLABO software to connect the instrument to a PC: ask your dealer for further
details.
❷ Cooling fan.
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The instrument is equipped with an auto sensing power supply; there is NO NEED
OF REGULATION between 110V and 220V, 50 and 60 Hz independently.
The master motherboard can operate with two 24K bytes EEPROM chips, allowing
QC data, result memory and methods setting to be stored inside.
In the incubator there are 2 Peltier cells, driven in parallel for thermoregulation by the
microprocessor. The Peltier cells work like a heater or cooler in accordance with the
direction of the current, so they are connected through a H-Bridge
(Q11,Q12,Q15,Q16) that can change the direction of the current that passes through
the Peltier in accordance with the MCU control signal.
There are 2 LEDs in the mother board for a visual signaling, if the voltage across the
Peltier cells is in positive or in negative ( when the cells are working as coolers the
green LED (LD4) is on, when they are working as heaters the red LED (LD5) comes
on).
When both the LED are off, this means no voltage is present in the Peltier cells.
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2.TECHNICAL CHARACTERISTICS
Photometric system:
Light source: long life halogen lamp 20 W
Spectral field: 340 nm - 690 nm
Filter wheel: automatic by motor
Filters: 340, 380, 405, 505, 546, 578, 630, plus one empty
position; pass-band 6 nm
Detector: at solid state
Thermostatic control:
Heating element: refrigerating and heating Peltier cell
Temperature: 20 to 40 °C
Temperature precision: + 0,1 °C
Stabilizing time: 10 minutes
Thermostatic block: at 10 positions per square or round test tubes, macro
and semimicro
Flow system:
Flow cell: 80 µL two ways
Typical working volume: 500 µL
Minimum working volume: 350 µL
Carry over: lower than 1%
Aspiration: peristaltic pump with programmable sipping volume
Measuring system:
Reset: automatic
Measuring range: -0.200 +2.500 OD
Photometric linearity: + 1 % from 0 to 2.000 OD
Photometric accuracy: + 1 % from 0 to 2.000 OD
Precision: + 1 digit
Drift: lower than 0.005 O. D. per hour
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Physical characteristics:
Power supply: 220 V - 110 V, 50/60Hz
Capacity: 160 VA
Weight: 11 Kg
Dimensions: H. 35 cm - W. 34 cm - D. 24 cm
Serial connection:
The output connector, standard 9 poles, is located on the rear of the instrument. The
connections are the following:
pin 2: input data.
pin 3 output data.
pin 5 reference signal.
The connection with serial input of a personal computer type I.B.M. or compatible
can be effected by a cable with direct connection of the three pins above described.
To verify the correct functioning of all mechanical and electrical parts, the instrument
has some utility programs.
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They can be selected interacting with user-friendly software of the instruments. The
following picture shows the basic layout of the display:
Date
Software Version
V 1-x.xx 18/10/04 12.35.56 Time
Temperature Window
Top Window:
shows the status of
the machine
Body Window:
shows results and the
parameter actually Menu window
selected
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The main service menu appears like this window (English language),
To enter the Service Menu, press MENU when you are in Main Menu, then select
“SERVICE” using DOWN arrow and press ENTER. The Body Window appears as
follows:
ABS Mode
Pump Calibration
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ABS Mode
340
Use arrows to select the filter, then press ENTER to confirm and go to the next
setting (Temperature and aspiration volume).
On the contrary press STOP to exit from ABS mode. When you completed the
settings, on the Body Window will appear the message “INSERT BLANK”. Insert the
blank cuvette into the reading cell and press ENTER. Then will appear the message
“INSERT SAMPLE”: insert the sample cuvette into the reading cell and press
ENTER. The absorbance value will continuously be shown until STOP key is
pressed. This method, applied to sample control solutions with known absorbance
value at a specific wavelength, can be used to verify the instrument accuracy.
The peristaltic pump is moved by a stepper motor which permit to intake the volume
of sample with high level of precision.
WARNING This calibration should be carry out periodically, because variation in the
elasticity of peristaltic silicon tube could vary the quantity actually sipped.
5 mL
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Procedure:
- Select the Pump calibration program.
- Put the aspiration spout into a cuvette with 5 ml accurate of distilled water.
- Press the push button. The peristaltic will begin to work.
- When the target quantity has been aspirated (5 mL) press immediately the push
button. The LCD display the value in ms of the time that occurred. This value is
stored and is used as reference for the instrument to intake any quantity of
sample. Optimal value goes from 8000 to 14000.
- The instrument will return automatically to main menu after few seconds.
Value:
less than 8000 abnormal motor running. Too fast or wrong tube.
8000- 14000 the rubber is efficent and in good condition.
Bigger than 14000 Obstruction on the aspiration spout or tube consumed.
You can reach “Diagnostic & Service” selecting “Diagnost. & Service” in the Service
Menu. The Body Window will appear as follows:
Quick Diagnostic
Service Only
If you select “QUICK DIAGNOSTIC” the analyser will print some self-diagnostic
parameters, which colud be helpful when you call service to solve any problem.
This section will explain the meaning of the parameters printed by “Quick diagnostic”
mode.
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Service only
Host programming
Hardware Test
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Host programming:
Host programming is useful for transferring data to the instrument and setting up the
method inside the 120 test memory (this is useful for customizing most of the method
parameters, like K-Factor, test name, standard concentration, number of decimal
point and so on).
You can use this program also to restore factory settings (in case of memory
corruption). You need a special program (called HM3000.exe), running in Microsoft
Windows (c), to transfer the method file and parameters to the instrument.
Refer to chapter 5 on how to use this program to connect it with the instrument.
This program is also used to upgrade the Slave MCU (in case it is needed), by the
mean of a special program (refer to BIOLABO website or contact BIOLABO service
engineer for further info).
This program is not suitable for upgrading the Master MCU (you MUST use, in this
case, the Bootloader, so you have to start with the instrument OFF).
Hardware Test
NOTE:
In order to work with this program you have to connect EXTERNAL PS-2 keyboard.
Otherwise you will never access to the basic hardware function of the instrument.
Be aware that matrix keyboard do not produce any command (except double click
when STOP is pressed) and WASH (to verify instrument washing), in order to make it
possible to diagnostic it.
Only PS2 code is allowed in this program.
To check if the PS2 keyboard is working it is enough to press NUM LOCK key and verify the
corresponding LED status (ON and OFF).
NOTE:
PS2 key are KEY sensitive. So it MAKES different from command capital and small
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KEY FUNCTION
b Buzzer test
T Temperature calibration (Warning: special device is needed!!) DO NOT PERFORM WITHOUT
SPECIAL TOOLS
R Execute automatic and continuous reading on the selected filter
1-8 Makes filter selection:
1 = 340
2 = 405
3 = 380
4 = 505
5 = 546
6 = 578
7 = 630
8 = Empty
H Execute filter wheel homing and realignment
p Printer test
0 Test Serial port to PC
V Burn in mode for instrument (term cycle) (TEST PROGRAM)
L Set linearity compensation values. (Warning: special device is needed!!) DO NOT PERFORM
WITHOUT SPECIAL TOOLS
C Execute instrument calibration
D Test/Set RTC (Real Time Clock): date and time
f Test ON/OFF fan
v test ON /OFF lamp
B Execute automatic bichromatic filter cross check. (TEST PROGRAM)
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1= Modify ‘AMAX’.
2= Restore of the EEPROM and HEADER factory setting (in case of corruption of memory)
3= Temperature offset adjustment (q).
NOTE: To decrease temperature, increase the offset, to increase the temperature, decrease
the offset.
Refer to chapter 4, section ADJUSMENT OF TEMPERATURE MESUREMENT
Setup program
In this program it is possible to set the some print characteristic, the language
regarding the display message and to active the air gap choosing the quantity of air
and the delay time between the sample aspiration and air aspiration, and so on.
The air gap could be introduced to save a small quantity of sample, actually to
reduce the carry-over effect it must be aspirated a volume of sample enough to fill
the flow cell and all the way from the aspiration spout, so is possible to intake the first
part with the sample to fill the flow cell and following part with air to fill the tube.
WARNING Pay attention to set the quantity of air not too big otherwise the air enter
in the flow cell.
This will lead to incorrect result and reproducibility problems.
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PRELIMINARY PROCEDURE
Before performing the following operations, make sure that the instrument is
switched off, open the instrument unscrewing the four screws from the bottom that fix
the cover on the basis and place the cover on the right side without turning it over in
order to be able to operate on the keyboard.
BATTERY REPLACEMENT
You need to replace the battery when you have a malfunctioning on date and time.
The nominal voltage value at battery pins cannot be less of 3V.
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Reading
aperture of the
photometer
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5. It is possible that after having centered the lamp, there is too much energy on
the preamplifier board. You need thus to perform PREAMPLIFIER BOARD GAIN
ADJUSTMENT (see the related section in this chapter).
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The preamp board has a bias current due to the offset of its operational amplifier.
This current has to be compensated in order to have correct results.
First of all, perform preliminary procedure to open the instrument. Then connect a
multi meter to test points TP2 and TP5 on the mother board (see Appendix 1). These
test points are directly connected to preamp output: you have to connect the red tip
of the multi meter to TP2 (OFFSET value) and the black tip of the multi meter to TP5
(GND).
Then switch on the instrument and insert the cuvette into the reading hole. Rotate the
cuvette for 90°, in order to have the dark face of the cuvette in front of the
photometer. Select the best resolution you have on your multimeter (you need at
least 0.1 mV for resolution). Act on trimmer RV0 of preamp board (see following
picture) until you reach a value between 0.5 mV and 1mV.
NORMAL ROTATED
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FILTER CHECK
1200
SAFE RANGE
8000
ADC
OUTPUT
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If filters’ transmittance values are not all in the safe area, you should adjust the gain
of the preamp board (ADC converter). Enter “Hardware Test” and press “R” in PS2
keyboard to execute a continuous reading of the transmittance values of the filters.
Press “1” in PS2 keyboard to select filter one (340 nm) and check the value if it is not
Trimmer RV1:
GAIN
in the safe area (1200-8000), you have to act on trimmer RV1 of preamplifier board
until the value is not in the correct range.
Repeat the operation for all the seven filters (you can select a filter pressing the
correspondent number on the PS2 keyboard).
If for one or more filter is not possible to reach Safe Area, you need to add or remove
the gelatin coating or to replace the filter (see next pictures as examples and next
paragraph for description).
1 2 3 4 5 6 7
1200
ALL in SAFE AREA
8000
340
630
ADC Output
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1 2 3 4 5 6 7
1200
Filter 3 is not in safe area:
remove some gelatin
coating to increase energy
value
8000
340
630
ADC Output
1 2 3 4 5 6 7
ADC Output
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FILTERS REPLACEMENT
If you need to replace some filter, first of all you have to perform preliminary
procedure.
Then unscrew the four screws which tie the two stirrups of dissipater block to the
basement.
Disconnect all the cable of the entire block from the motherboard and remove the
entire block (dissipater + incubator) from the instrument.
Then unscrew the four screws shown in the following picture to separate filter wheel
block from the incubator.
Then rotate filter wheel by hand, until the hole on the bracket coincides with the one
on the filter wheel.
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Use a 1,5 mm spanner to loosen the grain and liberate the filter wheel. Be sure that
the little washer is coupled with the stepping motor shaft.
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With a screwdriver remove the fixing washer and replace the filter paying attention
that the mirror face is turned downwards, furthermore pay attention to the gelatins
under the filter (for 340 nm filter no gelatin is present). AVOID touching the 2 optical
surfaces of the filter and the gelatins by hands not to soil them.
After the replacement, remount all the parts and perform preamplifier board gain
adjustment.
1 340 Nothing
7 630 2 gel of 3
8 free -
Equalization to be used as starting point for filter coating. May changes according with
filter transimattance, age, lamp emission photodetector spectral response.
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The motherboard doesn’t need any hardware calibration, because they have been
carried out by the constructor. Please do not act anyway on trimmer RV1 and RV2 on
motherboard: these trimmers have been factory pre-setted and hardware calibration
during the motherboard quality control process.
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Perform the preliminary procedure, in order to access to the peristaltic pump tube.
You can replace peristaltic pump tube by simply detaching it from the plastic tip
connected to the TEFLON tube.
See the figure below for a schematic view of the pump:
PERISTALTIC PUMP
OUT
WARP DIRECTION
TEFLON
IN
PLATE
To change the tube disconnect the elastic tube from its 2 adapters, by simply pulling
them out.
Then you can replace the tube and reconnect the 2 adapters.
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Before closing the cover again, be sure that the peristaltic pump elastic tube has a
suitable strength. You can easily check for this by aspirating some distilled water and
see if anything drop form the intake tip outside the instrument. No drops must be
present.
PUS
No drops present!
If you have still some drops, despite having already changed the tube, you must
insert some plastic ring (shipped with the tube) in order to increase the strength of
the elastic tube.
Usually inserting one on the top and one on the bottom it is enough for adding the
missed power to the peristaltic pump. Refer to the figure below for the correct way of
placing this 2 rings:
Remember that if you don't have the need to add this ring, don't add them to the
tube, because they increase the strength, reducing the lifetime of the peristaltic pump
tube.
NOTE: You must recalibrate the peristaltic pump aspiration volume after having
changed on of the part above.
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To perform this operation you don’t need to open the instrument, so you don ‘t have
to perform preliminary procedure: the operation will be carried out via software. Insert
a thermocouple thermometer into reading cell and swicth it on. Next picture shows
the thermocouple thermometer.
CAP 37.0
THERMOCOUPLE
CUVETTE
1000 uL WATER
THERMOMETER
NOTE: Be sure that your thermocouple thermometer is calibrated and with 0.1 °C
of accuracy in measurements. If you set temperature at a wrong value Kinetic
analysis results will be mistaken.
NOTE: If you use mercury thermometer you should insert it in a cuvette filled with
water NOT DIRECTLY IN THE INCUBATOR. You need 0.1 °C of accuracy in
measurements. You need more time to reach a stable temperature with a mercury
thermometer, since it affects the measurement greater than a thermocouple (typically
the waiting time are doubled, i.e. 1h.)
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Connect a PS-2 keyboard to the instrument and switch it on. Enter “Service Only”
Menu as explained in chapter 3 and select “Hardware Test”. Press “Q” key on PS-2
keyboard to enter hidden command program. The display will show only the script
“Press ENTER to return!”. Don’t press ENTER but press “3” key to perform
temperature offset adjustment. Press ENTER as the instrument ask you to confirm.
Increase or
Decrease?
Yes=Inc. NO=Dec
You have to press “YES” or “ENTER” to increase the offset value or “NO” or “ESC” to
decrease it.
Increasing offset will decrease the temperature value, decreasing offset will
increase temperature value.
Depending on your choice, the display will show one of this window:
- 0.00 + 0.00
With arrows and number keys, choose the value to increase or decrease offset value
and press ENTER. The display will show the script “>>>>> OK! <<<<<”: press
ENTER again.
Wait until the temperature value on your thermocouple thermometer become stable.
Repeat the operation until the temperature value reachs 37 °C. Then exit from
“Hardware Test” and press ENTER as the instrument ask if you want to save the
changes.
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Heating elements (Peltier Cells) are located under the incubator and fixed to the heat
dissipator in two proper lodging.
Perform the preliminary procedure to open the instrument. Then extract dissipator-
incubator block as explained in “Filters Replacement” section.
Now unscrew the four black screw which tie incubator to the dissipator. The following
picture shows two of these screw, on the opposite side you will find the others.
Then pull up the incubator: next picture will show the two blocks separated (in this
picture also the lamp has been removed)
Thermal
conductive
PAD
Peltier
Cell
Peltier
Cell
Replace Peltier cells according to the picture above. Remount incubator on the
dissipator: you can remove one of the two lateral stirrup to perform the operation
easier. Be sure to screw the four black screw of the incubator so that pulling peltier
cables doesn’t make the cell to move.
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The Peltier are coupled to the incubator and the heatsinker with a thermic conductive
PAD. This PAD ensure good heating transmission and increase heating and cooling
efficency.
Assemble the Peltier cells in this way:
Incubator
Pink thermic
conductive
PELTIER CELL PAD
Important:
Avoid thermal short circuit to increase heating efficiency. In case there is not heating
or cooling efficiency a parasitic thermal path may be present.
GOOD NO GOOD
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5. INSTRUMENT REPROGRAMMING
This section will explain how you can reprogram the instrument
REPROGRAMMING METHODICS
It is possible to reprogram all the methods via PC, if you need to personalize analysis
parameters or if you need to restart with default parameters.
For further details contact BIOLABO:
With Header Programmer software it is possible to modify via PC the name of the
instrument printed when it is turned on and to personalize the report printed with test
results in “Result Printout” operation. You can reach “Result Printout” from “Print”
Menu.
Connect the instrument to the PC through a serial cable (RS-232). Switch on the
instrument and enter “Host Programming” as explained in chapter 3.
Run Header Programmer from your PC: on your display will appear the software’s
window as follows:
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For spare parts list, visit section “SPARE PARTS LIST” in our website www.biolabo.fr
and follow the link spare parts.
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TP2 J16
TP
5
J5
J6
J9
J11
J15
J8 J3
J17
J2
J12
J18
J10 J14
J7
J4
J20 Pag 45 of 55
J1
Biolabo Service Manual
This section will show all voltage values and checkpoints for KENZA MAX
Biochemistry motherboard:
Pin 1: +15 V
Pin 2 : GND
Pin 3: -15 V
Pin 4: +5V
Pin 5: GND
Pin 6: NC
Pin 7: +21
Pin 8: GND
J4: LAMP
Pin 1: 10V
Pin 2: GND
Pin 1: +12V
Pin 2: GND
Pin 3: -12V
Pin 4: 0V to 10V
J6: TEMPERATURE
Pin 1: +12V
Pin 2: GND
Pin 3: NC
Pin 4: Temperature (°C) = Vout (mV)/10
Pin 1: 3V to 5V
Pin 2: GND
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Pin 1: GND
Pin 2: +5V
Pin 3: NC
Pin 4 – Pin 16: LOGIC SIGNALS
Pin 17- Pin 18: NC
Pin 19:LOGIC SIGNAL
Pin 20-Pin 21: NC
Pin 22: -17V to –10V (Contrast setting voltage)
Pin 23: GND
Pin 24: NC
J9: LED
Connector absent
J11: BUZZER
Pin 1: +5V
Pin 2: +5V normally, 0V when buzzer is on
Pin 1: NC
Pin 2: Host_TX (Standard RS-232 voltage values)
Pin 3: Host_RX (Standard RS-232 voltage values)
Pin 4: GND Float
J13: PRINTER
Pin 1: +5V
Pin 2: +5V
Pin 3: PRN_TX (Standard RS232 voltage values)
Pin 4: PRN_RX (Standard RS232 voltage values)
Pin 1: +5V
Pin 2: +5V
Pin 3: GND
Pin 4: Standard PS-2 voltage values
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J15: KEYBOARD
Pin 1: +12V
Pin 2: +12V / -12V
Pin 1: 0V / 5V
Pin 2: 0V / 5V
Pin 3: 0V / 5V
Pin 4: 0V / 5V
Pin 1: +5V
Pin 2: 0V / +5V
Pin 3: GND FLOAT
Pin 4: NC
J19: PELTIER
Pin 1: 0V / +5V
Pin 2: 0V / +5V
Pin 3: 0V / +5V
Pin 4: 0V / +5V
Pin 1: +5V
Pin 2: GND
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APPENDIX 2: TROUBLESHOOTING
This appendix shows the error messages related to issues that the user can normally
solve by himself: if the problem persists, or a problem not listed below arise, contact
your dealer.
Excluding the main plug fuses, the instrument has no user serviceable parts:
only trained technicians are allowed to service the instrument. An
unauthorized action on the instrument may invalidate its safety and features,
beside void the warranty.
In case of suspect maflunctioning of the instrument, we recommend to check the
instrument with coloured solution or control serum of known value.
In the following table we describe messages and flags which appear on the Body
Window or in a window which appears in the middle of the display:
ERROR -1 The sample absorbance is too high or the Press ENTER to repeat the reading otherwise
reading is impossible, due to faulty instrument dilute the sample. If message persists call our
service technicians.
Printer error Error when printing Press STOP to disconnect the printer, or any
other key to try again.
Temperature The thermostat has not yet reached the required Wait.
Window is temperature.
blinking
Temperature The temperature is disenabled
Window shows
----
Const ERROR Critical error in EEPROM Call service.
LLLL In an MSD analysis, the sample concentration is The sample concentration is lower than the
less than that of the lowest standard. minimum range of the method.
CALIBRATE It appears when you select an EP analysis with Press ENTER if a new calibration is required.
(Y/N) standard or FXT with standard. It allows the The instrument will be ready to read the
possibility to select a new calibration with the standard: INSERT STANDARD. Press STOP if
standard or read directly the sample cuvette a new calibration is not necessary. The
instrument is ready to read the sample:
INSERT SAMPLE.
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D The analytical result exceeds the linearity limit of Dilute the sample
the reagents.
* The thermostat has not reached the right Repeat the analysis when the thermostat and
temperature when the analysis was performed the reagents have reached the right
(blinking temperature window). The result temperature.
therefore, is not correct.
L The analytical result is less than the lower limit
value of the method.
H The analytical result is more than the higher limit
value of the method.
In the following table, we describe Error Messages which appear in the Error Message Area
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Carry-over is the residual solution inside the flow cell that affects the final result of a
photometric measurement. This quantity depends by several factors, but the most
important for our purpose are:
• The density of the solution used
• The absorbance value of the solution
• The internal cleanliness of the flow cell
• The quantity of aspirated volume (sample volume)
• The flow cell volume. The instrument can work either with 80 uL flow cell (that is
the standard flow cell) or with the 18 uL flow cell (upon request).
The instrument is shipped with its carry-over value indicated in the documentation
that refers to its internal Quality Control. Instrument carry-over must be less than 1%
to pass the Quality Control.
Air-gap can reduce dramatically carry-over effects, but requires a little of experience
in working with it. Air-gap is an instrument features that allows the User to set up a
Sample volume, a Delay time and an Air anspiration volume. Basically the instrument
performs a DOUBLE ASPIRATION (the first for the sample, the second for the air).
In fact, after each sample the peristaltic pump is turned OFF for a programmable
amount of time (User should remove the reading solution from the aspiration
spout during this delay) and is turned ON again for a certain amount of time in
order to aspirated a programmable volume of air.
This parameters are General Setup Parameters (not test parameters) so will remain
the same for all the test.
DELAY = 00 s
ANSP. VOL. = 00000 uL
The instrument is shipped with this 2 parameters disabled (their values are set to
0000).
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You just have to enter “Setting” in “Setup” Menu to set this 2 parameters (They are
called “Air aspiration” and “Delay”).
According to the test you are executing and to the test manufacturer you can change
these parameters in order to fit their best values.
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The analyzer is shipped with the reading height that exactly matches the reading
aperture in the flow cell (the same height for 18 or 80 uL flow cell). In this condition
the analyzer can work correctly in the flow cell and with a minimum reading volume of
1500 uL in standard plastic cuvette (10 mm optical path), like the ones supplied with
the instrument.
Some kits however have a reading volume of less than 1500 uL (for example 1000
uL). With this kit is no possible to work with the instrument unless:
1) Use reduce volume cuvette (still 10 mm optical path). Contact your nearest
distributor for more information.
2) Adjust the PVC screw in the incubator chamber in order to increase the reading
height. In this way you can reduce the volume according to your needs, but
remember that the light beam does not match anymore the flow cell reading
aperture and you have to setup it again if you want to work again with flow cell.
The figure below explains how to adjust the reading height (regulating the height of
the PVC screw) in order to work with flow cell or reduced volume. Refer to the
following table for more information:
PVC SCREW
*: Note that the screw height is measured according to the turn from the down
position.
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NOTE:
Use maximum care when performing this operation, since an excessive strength may
damage permanently the PVC screw inside the reading incubator chamber.
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In the SERVICE menu, select “Diagnostic & Service” and press ENTER.
ABS Mode
Pump Calibration
Than select “Service Only” and press ENTER. Finally select “Hardware Test” and
press ENTER.
Press “P” in the PS2 keyboard (be sure to press “P” because “p” would start printer
test), the instrument ask you if you want to lock methods: press ENTER to confirm.
The display will show four digit to insert a password:
____
Following the same procedure and inserting the correct password will allow you to
unlock Methods.
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