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Picture palang nangangamoy baho na huhuhu men
1. RNA is easily damaged by enzymes?
Why does RNA hz0nm than DNA? ^ i also think so ? (2)
a. RNA is easily damaged by enzymes
b. RNA contains a lot of impurities
c. RNA is easily undergoes hydrolysis
d. RNA is a single strand nucleotide
2. No brick-red precipitate? Kasi non-reducing

What is the expected result if a sample sugar ang sucrose
containing sucrose is subjected to ^ agree
Benedict’s test?
A. Muddy brown solution
B. Presence of brick-red precipitate
C. Blue-green solution
D. No brick-red precipitate
3. Selwanoff’s test & benedict’s test?

A sample containing lactose is subjected to Benedict ba? Monosac lang benedict yata?
different qualitative tests. What is/are the A and B yata kasi mali rin D dapat may
expected result(s)? crystal?
A. Seliwanoff’s test: faint pink solution Hindi ba B lang?
B. Bial’s orcinol test: blue-green
solution
C. Benedict’s test: brick-red precipitate
D. Mucic acid test: no crystals
4. 4.25 M
The glucose content of samples can be
determined using Nelson’t test. The 0.38M akows
equation of the line for the standard curve y=3.0x + 0.2
prepared for varying concentrations of y=1.35
glucose is y= 3.0x + 0.2. Based from this 1.35=3.0x +2
information, determine the glucose 1.35-0.2 = 3.0x
concentration (in M) of a strawberry 1.15/3=3.0x/3
preserve having an absorbance reading of =0.38M
1.35.
A. 0.38 M
B. 3.27 M
C. 4.25 M
D. 2.18 M
A sample containing an unknown glucose?

carbohydrate is subjected to different
qualitative tests. What is the possible
carbohydarte present given the following
results?
Benedict's test: brick-red precipitate
Seliwanoff's test: slow reaction
Bial's orcinol test: muddy brown
solution
Mucic acid test: no crystals
1. Glucose
2.
3. Sucrose
4.
5. Xylose
6.
7. Fructose
Determine the carbohydrate described in
each number.
5. Glucose?
A sample containing an unknown
carbohydrate present given the following
results?
- Benedict's test: brick-red ppt.
- Seliwanoff’s test: slow reaction
- Bial’s orcinol test: muddy brown
sol’n
- Muric acid test: no crystals
A. Fructose
B. Glucose
C. Xylose
D. Sucrose
6. Orcinol test
Which of the following tests produces a âgree
furfural derivative?
A. Pyrimidine test
B. Dische test
C. Orcinol test
D. Murexide test
+,+,-,+,+,Higher? Hindi po ako sure sa

sagot ko
Halp
hAAAAAALP:<
7.
The above molecule was subjected to acid
hydrolysis then was analyzed thru qualitative
tests. Indicate wether the given molecule will
give a positive (+) or negative (-) result in
each given test. (All or nothing)
Identify wether (+/-)
Test for phosphate Blank 1

Dische Test Blank 2
Bials Test Blank 3
Murexide test Blank 4
Wheeler-Johnson Test Blank 5
Transitory temperature: Higher or lower

Blank 6
8. Which of the following reagents/method Help :<<<<

can be used to break the histone-DNA Sodium chloride? Huhu not sure din
Feel q yung sodium dodecyl sulfate bcos
complex? It breaks ionic interaction between protein
and nucleic acid and histone is protein?
1. Sodium dodecyl sulfate
2. Sodium chloride
3. EDTA
4. Heating
9. Homogenization?
Dissociation ~?
Step in DNA isolation that involves
precipitation of nucleic acid;
1. Homogenization
2. Purification
3. Dissociation
4. Preservation
------------------------------------
A.
Excess sulfuric acid in test for phosphate Heating

can be eliminated by:
B. Addition of saturated bicarbonate
solution
C. Addition of water
D. Addition of alkali solution
E. heating
10. Cytosine + Guanine?

An increased melting temperature of DNA ^same
results from a high content of:
A. Thymine + Cytosine
B. Cytosine + Guanine
C. Cytosine + Adenine
D. Adenine + Guanine
Why does T gives a negative result for Due to the presence of Methyl group at C3
Wheeler-Johnson Test
????? not sure
1. None of the above

2.
3. Due to the presence of Methyl group
at C5
4.
5. Due to the presence of Methyl group
at C3
6.
7. It is an oxidizing agent
11. Adenine and Guanine

Name those purine bases which are
commonly found in DNA and RNA?
A. Cytosine and Thymine
B. Adenine and Thymine
C. Cytosine and Guanine
D. Adenine and Guanine
12. Molisch - H2SO4, ethanol, alpha naphthol

What are the reagent used in the following Benedict - CuSO4, Na2CO3, Na3C6H5O7
tests for carbohydrates Seliwanoff - HCL resorcinol
Muric - conc. HNO3
Prompts Nelson A - Rochelle salt, Na2CO3,
Molisch’s Test NaHCO3, Na2SO4
Benedict’s Test Kulang ba choices - sorry omg nadistract
Seliwanoff’s Test ako HAHAHAHA
Mucic Acid Test Izzokie!!!! :> <3
Nelson’s Test (Reagent A)
Choices:
COncentrated HNO3
CuSO4, Na2CO3, Na3C6H5O7
HCl, resorcinol
H2SO4, ethanol and alpha-napthol
Rochelle salt, Na2CO3, NaHCO3, Na2SO4
13. 2?
7.JPG ^same
Which of the following structures is a hexose?
14. Dehydration followed by condensation???

What is the principle involved in Molisch’s ^yes i think so too
Test?
A. Dehydration followed by condensation
B. Complexation
C. Precipitation
D. Redox
15. Uracil
Name the pyrimidine base which is found in
RNA but not in DNA?
A. Adenine
B. Thymine
C. Guanine
D. Uracil
16. 4
^same
Which of the following structures is a
ketose?
17. Ketopentose?
Chrue????
What is the classification of the Di ba aldopentose kasi nasa huli yung
monosaccharide? carbonyl??????
^yes i think bcos aldehyde is terminal?
2(1).JPG âgree sa aldopentose
1. aldopentose
2. aldohexose
3. ketopentose
4. ketohexose
18. Glucose?
Same?
A sample containing an unknown
^same
carbohydrate present given the following
results?
Seliwanoff's test: slow reaction
solution
1. Fructose
2. Glucose
3. Xylose
4. Sucrose
19.
What is the role of cupric sulfate in Nelson's
test?
Oxidation agent
Make sugar reactive
Dissolves cuprous oxide
Prevents precipitation of cupric hydroxide
20. Nucleotides
The monomeric unit of nucleic acid are called;
1. Nucleotides
2. Pyrimidines
3. Nucleosides
4. Purines
Which of the following reagents results to Amylase??

incomplete polysaccharide hydrolysis?
1. strong acid
2. amylase
3. strong base
4. water
What is the principle involved in Nelson's redox?

test?
1. Dehydration followed by condensation
2.
3. Complexation
4.
5. Precipitation
6.
7. Redox
Which of the following is FALSE about purine Help

At acidic or alkaline pH the bases become
and pyrimidine bases?
charged and their solubility in water
1. Purines have two rings in their increases?????????????
Noo that’s true according to google :(
structure, but pyrimidine bases have
Pati ung hydrophobic sila
only one ring
2.
3. At acidic or alkaline pH the bases
become charged and their solubility in
water increases
4.
5. They are hydrophobic and relatively
insoluble in water at the near-neutral
pH of the cell
6.
7. Purines have two rings in their
structure, but pyrimidine bases have
only one ring
21. 10% trichloroacetic acid?

Which of the following reagents is used to
remove the proteins in liver by precipitation?
1. 10% trichloroacetic acid
2. sodium chloride
3. copper (II) sulfate
4. 95% ethanol
22. Absorption at UV light
âgree
Purity of nucleic acids can be analyzed
experimentally by;
1. Absorption at Visible light
2. Qualitative test tube test
3. Absorption at UV light
4. TLC
23. 1?
Âgree broad/flat crystals
What is the expected result if a sample
^sameee
containing galactose is subjected to osazone
test?
1. broad/flat crystals
2. broken glass-like crystals
3. needle-like crystals
4. fine needle crystals grouped in balls
24. 3, 2, 1?
The following carbohydrates were ÔH ^Polar
subjected to TLC analysis using n- butyl ^Polar lower rf value pacheck hehe
alcohol: acetic acid: ether: water (9:6:3:1
v/v) as mobile phase and 3,2,1 po???
para- anisaldehyde as visualizing agent.
Arrange the carbohydartes in increasing Rf
value.
1. 3, 2, 1
2. 2, 1, 3
3. 2, 3, 1
4. 1, 2, 3
25. 4.25 M
The glucose content of samples can be Hindi ba 0.38?
(1.35 - 0.2) / 3.0
determined using Nelson't test. The equation
absorbance = (slope)(concentration) +
of the line for the standard curve preapred for y-intercept
varying concentrations of glucose is y = 3.0x
+ 0.2. Based from this information, determine
the glucose concentration (in M) of a
strawberry preserve having an absorbance
reading of 1.35.
1. 0.38 M
2. 3.27 M
3. 4.25 M
4. 2.18 M
C?
26. Same kasi condensation with resorcinol
What is the principle involved in
Seliwanoff’s Test?
A. Precipitation
B. Redox
C. Dehydration followed by
condensation
D. Complexation
27. Dische -Diphenylamine in Conc. Sulfuric acid

Match the following test with the Murexide -Conc. Nitric Acid and Ammonia
chemical/reagents commonly employed for solution
the chemical characterization of Nucleic Pyrimidine -Bromine water
acids. Bial’s -Orcinol in Hydrochloric Acid
Prompts
Phosphate - Ammonium molybdate
Dische test
Murexide test
Pacheck po hehehehe
Pyrimidine test
Bial’s orcinol test
Phosphate test
Choices
Dinitrosalicylic acid
Conc. Nitric Acid and Ammonia solution
Diphenylamine in Conc. Sulfuric acid
Ammonium molybdate
Bromine water
Orcinol in Hydrochloric Acid
28. The bond between sugar and the

Which of these statements are TRUE for a
nitrogenous base
glycosidic bond in nucleic acid?

1. The bond between sugar and
phosphate
2. The bond between base and
phosphate
3. The bond between sugar and the
nitrogenous base
4. The bond between the two sugar
residues
29. 1.7-2.00 nakita ko pero close naman so

True??
Pure isolated DNA has a relative value of
I think false kasi hindi exact yung relative
1.8-2.0 value
F po kasi more than 1.8 may RNA
Tf
contamination na
30. 1?
In RNA molecule guanine content does not
necessarily equal its cytosine content nor
does its adenine content necessarily equal its
uracil content since it is a;
1. Single strand molecule
2.
3. Double stranded helical molecule
4.
5. Polymer of purine and pyrimidine
ribonucleotides
6.
7. Double stranded molecule
Blank 1 - 260 nm
31. Blank 2 - 280 nm
Nucleotide bases absorb light at Blank 1 nm,
while aromatic amino acids absorb light at
Blank 2 nm.
32-36.. Most abundant in our diet- Glucose

Sweetest of the carbohydrate- Fructose
Milk sugar-Lactose
each number. Major structural material of plants-Cellulose
Storage form of glucose- Glycogen
Feel ko tama na yann

Okii ty
Uu ty
37-42.
each number.
43. Hydrogen bond

Which bond is used to stabilize the double
helix of DNA?
1. Hydrogen bond
2. Hydrophobic bond
3. Covalent Bond
4. Ionic bond
44. increased; hyperchromic effect?

When analyzing heat-denatured DNA, it
exhibits Blank 1 UV absorbance as helpppp
compared to double stranded DNA. This
effect is called the Blank 2
45. Adenine?
^same
A purine with an amine (NH2) group on the 6th
carbon is
1. Guanine
2. Thymine
3. Adenine
4. Cytosine
46. c?
Why does RNA has higher UV absorption at
260nm than DNA?
a. RNA contains a lot of impurities
b. RNA is easily damaged by enzymes
c. RNA is a single strand nucleotide
d. RNA is easily undergoes hydrolysis
47. True
DNAse solution is viscous at alkaline pH
Tf
48. True? Cause yeast sya??

as higher
S. cerevisiae h
Yes nakalagay din sa handout na mababa
^???? Mababa alin? Higher kasi nakalagay
nuclear-to-cytoplasmic volume ratio. sksksks
Tf
49. False? Sabi kasi sa google ice cold ethanol

In isolation of NA, unwinding of NA is
helps the NA precipitate
False
achieved thru the addition of ice-cold ethanol.
Tf
50. F???
âgree
DNAse contains Barium, a divalent cation.
Tf
51. true?
Isopropanol can be used to precipitate RNA
T or F
52. 3?
^yas
What is the principle involved in Seliwanoff's
test?
1. Precipitation
2. Redox
4. Complexation
53. 2?
âgree
The primary purpose of EDTA is to;
1. Precipitate DNA
2. Chelate Ca/Mg ions in the DNAse
3. Chelate Ca/Mg ions in the DNA
4. Isolate proteins
54. T??
Absorption of proteins in UV-region is caused
by Proline due to its alpha- imino structure.
Tf
55. dissolves cuprous oxide??

âgree
What is the role of arsenomolybdate in
Nelson's test?
1. oxidizing agent
2.
3. dissolves cuprous oxide
4.
5. prevents precipitation of cupric
hydroxide
6.
7. make sugars reactive
56. False
Cetyltrimethylammonium bromide
CTAB stands for
Cethylinetrimethylammonium bromide
T OR F
57. B?
What is the principle involved in Iodine âgree
Test?
A. Redox
B. Complexation
C. Precipitation
D. Dehydration followed by
condensation
What are the reagents used in the following

tests for carbohydrates?
3(1).JPG Alpha 1,6?

What is the glycosidic linkage at the indicated
part on the structure?
Which of the following statement(s) is/are C and D?

true about mucic acid test? SELECT ALL
THAT APPLY
A. secondary alcohol is oxidized to ketone
B. primary alcohol is oxidized to aldehyde
C. primary alcohol is oxidized to carboxylic
acid
D. formyl functional group is oxidized to
carboxylic acid
HELP PO
HELP POOOO
The above molecule was subjected to acid

hydrolysis then was analyzed thru
qualitative tests. Indicate wether the given
molecule will give a positive (+) or negative
(-) result in each given test. (All or nothing)
Identify wether (+/-)
Test for phosphate Blank 1

Dische Test Blank 2
Bials Test Blank 3
Murexide test Blank 4
Wheeler-Johnson Test Blank 5
Transitory temperature: Higher or lower

Blank 6
What is the glycosidic linkage at the indicated Alpha (1,4)?

part on the structure? ^
4(1).JPG
SDS causes salting-out phenomenon that can F

precipitate proteins during isolation of NA.
T or F
What is the principle involved in Benedict's Redox?

test?
a. Complexation
b. Precipitation
c. Dehydration followed by condensation
d. Redox
Which of the following reagent precipitates Acidified ethanol?

RNA from the solution?
a. Ice cold ethanol
b. Acidified ethanol
c. Chloroform
d. Glacial acetic acid
What is the role of carbonates in Nelson's Makes sugars reactive?

test?
a. Dissolves cuprous oxide
b. Oxidizing agent
c. Prevents precipitation of cupric
hydroxide
d. Make sugars reactive
What is the role of sodium potassium tartrate Prevents precipitation of cupric hydroxide
~?
in Nelson's test?
^true
1. make sugars reactive
2. oxidizing agent
3. prevents precipitation of cupric
hydroxide
4. dissolves cuprous oxide
Which of the following reagents results to Is it strong base?Strong base

epimerization of aldoses and ketoses upon
hydrolysis?
1. strong acid
2. water
3. strong base
4. amylase
In murexide test, the addition of KOH in the F?

reaction mixture will turn the reaction from red
Oo, naoh dapat?
to yellow.
1.
T OR F
A sample containing fructose is subjected to HELP :<<

different qualitative tests. What is/are the Seliwanoff's test: faint pink solution
expected result(s)? Osazone test: needle-shaped crystals

Seliwanoff's test: faint pink solution
Barfoed's test: no brick-red precipitate
Osazone test: needle-shaped crystals
What is the principle involved in Barfoed's 3?

test?
ôxidation so redox?
1. Complexation
2.
3. Redox
4.
5. Precipitation
6.
Which of the following reagents is used to 95% ethanol???

remove the proteins in liver by 10% trichloroacetic acid
precipitation?
A. 10% trichloroacetic acid
B. sodium chloride
C. copper (II) sulfate
D. 95% ethanol
A good supernatant solution and separation ^help

of larger materials after homogenization is Centrif?
achieved through which of the following
laboratory technique? helpppp
A. Suction Filtration
B. Gravity Filtration
C. Centrifugation
D. Decantation
Denaturation of proteins in isolation of DNA D?
from plant samples can achieved by; Shore buh?
A. Adding lysing buffer
B. Maintaining the procedure in an ice
bath
C. Adding proteolytic enzyme
D. Adding homogenizing solution
What would be the positive result of purified It will form a blue complex?
nucleic acid isolated from yeast when treated
with Diphenylamine in conc. sulfuric acid
1. It will not give a positive result
2. It will form a blue complex
3. None of the above
4. It can be quantified at 595 nm
Which of the following reagent precipitates Acidified ethanol?

pacheck
RNA from the solution?
1. Glacial acetic acid
2.
3. Acidified ethanol
4.
5. Ice-cold ethanol
6.
7. Chloroform
A sample containing an unknown Xylose?

âgree

carbohydarte present given the following
results?
Seliwanoff's test: faint pink solution
Osazone test: fine needle-shaped
crystals
A. Glycogen
B. Xylose
C. Fructose
D. Starch
Which of the following statement(s) is/are true 2? And 4?

^sameeeee
about phenylhydrazone test?
1. primary alcohol is oxidized to
carboxylic acid
2. formyl functional group is oxidized to
carboxylic acid
3. primary alcohol is oxidized to
aldehyde
4. secondary alcohol is oxidized to
ketone
Why does T gives a negative result for Due to the presence of Methyl group at C5
Wheeler-Johnson Test?
Due to the presence of Methyl group at C5

It is an oxidizing agent
Due to the presence of Methyl group at C3
None of the above
In UV analysis of isolated DNA samples, 1?

^sameee
samples can be measured under 230 nm
because;
1. Impurities such as carbohydrates and
salts absorbs strongly at this
wavelength
2. Impurities such as proteins absorbs
strongly at this wavelength
3. Pyrimidine bases absorbs strongly at
this wavelength
4. Purine bases absorbs strongly at this
wavelength
Prompts Test for Deoxyribose- Blue complx

Test for Phosphates- yellow crystalline
Test for Deoxyribose Test for Ribose- blue green sol’n
Test for Phosphates Test for Purines - red residue
Test for Ribose Test for Pyrimidines - Purple coloration
Test for Purines Pacheck hehe
Test for Pyrimidines
Choices:
Blue-green solution
Yellow crystalline precipitate
Purple coloration
Purplish red-yellow solution
Blue complex solution
Red residue
Thymidine; 1 and 2?
I. is equal to the adenosine concentration in
double-stranded DNA.
II. is replaced by Uracil in RNA.
III. normally forms 3 hydrogen bonds with
adenosine.
IV. can participate in hydrophobic interactions
due to its methyl group.
1. I and II
2. I, II and IV
3. II, III and IV
4. I, II and III
Isopropanol can be used to precipitate RNA. true?

T or F
Which of the following reagents is used to C? NaCl?

âgree
precipitate glycogen by salting-out?
a. Cooper (II) sulfate
b. 10% trichloroacetic acid
c. Sodium chloride
d. 95% ethanol
Amylose can be isolated from the source by 3?

immersing in water. What is the mechanism
involved in this extraction technique?
1. precipitation
2. salting-out
3. selective dissolution
4. homogenization
What is the principle involved in Bial's orcinol B
test?
a. Precipitation
b. Dehydration followed by condensation
c. Complexation
d. redox
What is the principle involved in Mucic acid 3?

test?
ôxidation, so redox nga?
1. Precipitation
2. Complexation
3. Redox
Which of the following reagents is capable of D?

âcid hydrolysis ang complete hydrolysis so
complete polysaccharide hydrolysis?
strong acid
a. Amylase
b. Strong base
c. Water
d. Strong acid
What is the classification of the Aldohexose??

monosaccharide? ???????
1(1).JPG Seim?
Same too pero not sure if correct
A. Aldopentose
B. Ketohexose
C. Ketopentose
D. Aldohexose
The glucose content of samples can be Halp

determined using Nelson't test. The equation 2.87M
of the line for the standard curve preapred for
varying concentrations of glucose is y = 3.0x
+ 0.2. Based from this information, determine
the glucose concentration (in M) of a mango
preserve having an absorbance reading of
0.891.
a. 0.23M
b. 1.95M
c. 2.87M
d. 3.18M
What is the principle involved in Osazone

test?
1. Precipitation
2.
3. Dehydration followed by
condensation
4.
5. Complexation
6.
7. Redox
Lysozymes are used in plant DNA isolation T?

for the disruption of cell membrane. Di ba bacterial sha?
t/f
The following carbohydrates were subjected 2, 1, 3 ~?

same^
to TLC analysis using n- butyl alcohol: acetic
acid: ether: water (9:6:3:1 v/v) as mobile
phase and para-anisaldehyde as visualizing
agent. Arrange the carbohydartes in
increasing Rf value.
A. 2,1,3
B. 2,3,1
C. 1,2,3
D. 3,2,1
MISS LIITAN MO NAMAN PICTURE GRABE

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HOUSE RULE/S:​ BAWAL PO MAG CTRL-A,​ ​CTRL-F LANG PO DITO

HAHAHAHAHAHAHAH GAGO KAYO HAHHAHAHHAA

SA TASK :>>​ ​please

TANGINA NAKA NAPKIN SI KUYA HAHAHAHAHAHA

2. No brick-red precipitate? Kasi non-reducing

3. Selwanoff’s test & benedict’s test?

A sample containing an unknown glucose?

+,+,-,+,+,Higher? Hindi po ako sure sa

Identify wether (+/-)

Test for phosphate ​Blank 1

Transitory temperature: Higher or lower

8. Which of the following reagents/method Help :<<<<

Excess sulfuric acid in test for phosphate Heating

10. Cytosine + Guanine?

1. None of the above

11. Adenine and Guanine

12. Molisch - H2SO4, ethanol, alpha naphthol

14. Dehydration followed by condensation???

Which of the following reagents results to Amylase??

What is the principle involved in Nelson's redox?

Which of the following is FALSE about purine Help

21. 10% trichloroacetic acid?

27. Dische ​-​Diphenylamine in Conc. Sulfuric acid

28. The bond between sugar and the

glycosidic bond in nucleic acid?

29. 1.7-2.00 nakita ko pero close naman so

32-36.. Most abundant in our diet- Glucose

Feel ko tama na yann

43. Hydrogen bond

44. increased; hyperchromic effect?

48. True? Cause yeast sya??

49. False? Sabi kasi sa google ice cold ethanol

55. dissolves cuprous oxide??

What are the reagents used in the following

3(1).JPG Alpha 1,6?

Which of the following statement(s) is/are C and D?

The above molecule was subjected to acid

Identify wether (+/-)

Test for phosphate ​Blank 1

Transitory temperature: Higher or lower

What is the glycosidic linkage at the indicated Alpha (1,4)?

SDS causes salting-out phenomenon that can F

What is the principle involved in Benedict's Redox?

Which of the following reagent precipitates Acidified ethanol?

What is the role of carbonates in Nelson's Makes sugars reactive?

Which of the following reagents results to Is it strong base?Strong base

In murexide test, the addition of KOH in the F?

A sample containing fructose is subjected to HELP :<<

Bial's orcinol test: muddy brown

What is the principle involved in Barfoed's 3?

Which of the following reagents is used to 95% ethanol???

A good supernatant solution and separation ^help

Which of the following reagent precipitates Acidified ethanol?

A sample containing an unknown Xylose?

qualitative tests. What is the possible

Which of the following statement(s) is/are true 2? And 4?

Due to the presence of Methyl group at C5

In UV analysis of isolated DNA samples, 1?

Prompts Test for Deoxyribose- Blue complx

Isopropanol can be used to precipitate RNA. true?

Which of the following reagents is used to C? NaCl?

Amylose can be isolated from the source by 3?

HOUSE RULE/S: BAWAL PO MAG CTRL-A, CTRL-F LANG PO DITO

SA TASK :>> please

Test for phosphate Blank 1

27. Dische -Diphenylamine in Conc. Sulfuric acid

Test for phosphate Blank 1