Lactase Enzyme Simulation

By: Sophie Marin

Variables

Hypothesis: If the pH of the soil is above or below 7 then the Glucose production from
Lactase hydrolysis will decrease, in addition to the rate of reaction.
Independent variable: The pH of each solution.
Dependent variable: The rate of reaction for the Lactase enzyme will change according
the pH of the solution.
Baseline Data

Initial Graph
pH Glucose pH Glucose
concentration concentration
mg/dL mg/dL

1 0 8 500

2 0 9 459

3 60.4 10 167

4 262 11 15.5

5 485 12 0

6 494 13 7.87

7 498 14 0
Procedure

Constants:
● The type of enzyme
● Temperature at 37 C
● Initial amount of Lactase in mg/dL
● Time limit
Modified variables:
● pH of solution
Data Analysis

Mean: 210.63

Standard Deviation: 226.57

Standard error of mean: 46.25

95% confidence interval: -242.51 and 663.77

Glucose Production from Lactose Hydrolysis Graph
Graph of pH data
Explanation

The enzyme Lactase breaks down Lactose into useable sugar like Glucose; when
conditions are desirable the enzyme is able to make 500 mg/dL of Glucose. The
desirable conditions include a temperature of 37 C and a pH range of 4.9-8. As the pH
moves away from the desired range, the activity of the enzyme, Lactase, decreases.
According to the graphs the Glucose concentration is the greatest at a pH of 8, which
means the Lactase activity is greatest at this point.