Enzyme Activity Lab

Objective: To study the effect of the enzyme lactase on milk

• An enzyme is a substance that acts as a catalyst

in living organisms, regulating the rate at which
chemical reactions proceed without itself being
altered in the process.
• The biological processes that occur within all
living organisms are chemical reactions, and
most are regulated by enzymes.
Carbohydrates
Specificity
Lactose Intolerance
Glucose Detection Strip
Principle of Glucose detection strip
Also called as GOD POD method (Glucose Oxidase – Peroxidase method)
Material & Methods
1. Enzyme solution – Add and dissolve the lactase tablet
to 200ml distilled water.
2. Skimmed milk – Milk from which cream has been
removed.
3. Sucrose solution – Add 5 gram of table sugar to 100 ml
of distilled water. Stir to dissolve the sugar.
4. Denatured Enzyme Solution –
i. Place 20ml of enzyme solution into a test tube.
ii. Add 200 ml of water to a 500 ml beaker.
iii. Place the test - tube in the beaker.
iv. Place the beaker along with test-tube on a hot-plate
or burner.
v. Boil the water in the beaker for 30 min. Cool it down
to room temperature
Experimental Plan & Result