Collecting Good Quality NBS Samples - A Quick Guidebook

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COLLECTING GOOD QUALITY NEWBORN SCREENING SAMPLES ™ SP et hit tin, CHER | 7 Good, quality samples are IMMEDIATELY TESTED upon receipt at the NSC laboratory. 2 Newborns with POSITIVE results are urgently recalled, affording them the opportunity to avail of further testing, evaluation by a pediatric specialist, and provided with the appropriate treatment, all in a timely manner. 3 Newborns with confirmed disorders are thus SPARED from the harmful effects of their disorders, and are given the chance to live normal lives in spite of their conditions. SoMN gta lo|tlel2| [vii Jelrjolaly [flo] [ofa ln [aleiel@ieloly| s| in| 16 Jujele oie jeladsiay delenit LIL LL foils eisisy saa oar Site LO Coteau pean fic ‘res ans Crete Nan Sanaa Lt (I) All data fields in the filter card are filed out completely (fictitious data @ @ @ @ oe {AORN 9836672 ‘903™ 4 2018-07 on suzuatonviat ®@e@@e back GOOD QUALITY SAMPLE + Ensure that the baby is well-fed prior to collecting blood sample. « Prior to specimen collection, apply gentle massage on the baby’s leg or apply warm compress to the foot. + Make sure the two punctures made on the baby’s foot are close to one another to encourage pooling of blood. + When squeezing baby's foot to promote blood flow, do not forget to Telease baby’s foot from the squeeze to encourage capillary refill. + Only apply specimen onto the filter paper when sufficient blood has pooled on the puncture site (see picture above). + Collect at least four (4) large blood drops that fully saturates up wi) SCV the back of the filter paper. 3 e. @ & ak eee re ‘Ce eit =a 8 ¢ 20102 Bo zerbse / evoeo ? CONTAMINATED SAMPLE: 4 caused by: Applying several drops of blood to the same area on the filter paper. W, Tips to avoid it: NN y Ensure to apply one drop of blood at a time to one spot on the \ filter paper. If you feel the blood collected on the filter paper g C is inadequate, place additional drops on another spot on the ‘ filter paper, but make sure the blood drops do not overlap, ‘Aetna 14 ~\ -—~. (GE ) ‘ ) — oi 06-60-6102 eo we CONTAMINATED SAMPLE: Overlapping caused by: Applying excess blood to the filter paper, usually with a device. Applying blood to both sides of the filter paper. Tips to avoid it: Ensure to apply one drop of blood at a time to one spot on the filter paper. If you feel the blood collected on the filter paper is inadequate, place additional drops on another spot on the filter paper, but make sure the blood drops do not overlap. pastor Rndft 92 JOWIUPHOd CONTAMINATED SAMPLE: caused by: (1) Removing filter paper before blood has completely filled circle ot before blood has soaked through to the other side of the filter paper. y (2) Allowing filter paper to come in contact with gloved or ungloved hands or substances such as hand lotion or powder, either before or after blood specimen a collection. NS Tips to avoid it: (1) Allow blood to fully saturate the filter paper before removing it away fromthe baby's foot. (2) Make sure that baby’s blood does not come into contact with other objects laps orsubstances apart from the filter paper itself, PerkinElmer 226 EA seme ravens §3 2017-07 eo @ ey) o \wa _ one CONTAMINATED SAMPLE: caused by: Applying several drops of blood to the same area on the filter paper. Tips to avoid it: Ensure to apply one drop of blood at a time to one spot on the filter paper. If you feel the blood collected on the filter paper is inadequate, place additional drops on another spot on the filter paper, but make sure the blood drops do not overlap. CONTAMINATED SAMPLE: Serum Rings caused by: (1) Squeezing or “milking” of area surrounding the puncture site, (2) Venous blood collected with syringe and delayed dropping of blood in the filter paper. Tips to avoid it: \ Avold excessive squeezing of the baby’s foot during blood extraction. N Ensure to release the baby’s foot after each squeeze to promote ‘ean esas capilary efi CONTAMINATED SAMPLE: Diluted caused by: (1) Not wiping alcohol from puncture site before making skin puncture. (2)Allowing filter paper to come in contact with alcohol, formula, antiseptic solutions, water, etc., either before or after blood specimen collection. Tips to avoid its ( V Ensure that the alcohol placed on the puncture site is already dry before pricking. Keep all unnecessary liquids away from the sample collection and drying areas to avoid contamination due to spillage of liquids. CONTAMINATED SAMPLE: [nsec Marks caused by: Not safely securing the NBS sample during drying such that vectors such as flies or other insects become attracted to the blood on the filter paper and leave contaminating markings. Tips to avoid it: A Ay Dry NBS samples in a safe, secure place where they can be protected N C / from all possible contaminants such as animal vectors, fluids, dirt, lays and the like. CONTAMINATED SAMPLE: Redent Bites caused by: Rodents are highly attracted to the dried blood on the filter paper and will readily eat it when they can. Tips to avoid it: (1) During drying of sample, ensure to keep the sample in a safe, secure place. away fromall animal vectors that are attracted to the blood on the filter paper. (2) When endorsing the sample to the courier for send out, ensure that the sample is securely packed first with clean paper, then with a cellophane wrapper prior to being enclosed in the courier pouch. This will protect the sample from redent bites and other contaminants during storage and transit. -~y 77 t rie o) (a (oe @ Oc-e™! 707 MF LannRAnE / Z800LL MOT on 077 seuraunas oN “en gos -_\ es #F @ @ - ~—_- ~- _—- 60-eLoz ZZZL00L / LozozLo OT = gzz worsiuy UNFIT SAMPLE: caused by: Collecting specimen and applying it onto the filter card when too little blood is produced from the puncture site. a = ome \ \ 4 t ' ‘ . ‘@ r o' g i ‘@: ! ‘@ oees on pee mic 17annt rian MiAnenns Awe wns pom ar | s \ UNFIT SAMPLE: Insufficient Tips to avoid it: avoid it: Ensure that the baby is well-fed prior to collecting blood sample. Prior to specimen collection, apply gentle massage on the baby’s leg or apply warm compress to the foot. Make sure the two punctures made on the baby’s foot are close to one another to encourage pooling of blood. When squeezing baby’s foot to promote blood flow, do not forget to release baby’s foot from the squeeze to encourage capillary refill, Only apply specimen onto the filter paper when sufficient blood has pooled on the laps Puncture site, Let us all remember to strive to get GOOD QUALITY newborn screening samples the first time, EVERY time. Tegethier, we can werk té SAVE the (ives of cur newborns and give tiem the FIGHTING CHANCE te achieve theit

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