Science,

Republic of the Philippines

Technology and
Department of Education
Engineering
WORKSHEETS IN BIOTECHNOLOGY
Quarter 3: Week 5

RECOMBINANT DNA TECHNOLOGY

Biotechnology

- Grade 8

Alternative

Delivery Mode

Quarter 3:

Week 5

RECOMBINANT DNA TECHNOLOGY

First Edition, 2019

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Published by the Department of Education

Secretary:

Undersecretary:

Assistant Secretary:

Development Team of the Worksheets

Author: CORAZON V. WONG

1
Editors: IMELDA CONCEPCION B. MANUNTAG

Reviewer: ANABEL B. MEJIA (RMCHS)

Language Editor: MA. GINA A. ANAMA (RMCHS)

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Biotechnology 8 Worksheet No.: 1

_____________________________________________________________

Name: ____________________________ Section: ______________

Date: ____________________________ Score: ______________

Recombinant DNA Technology

MELC: Q3_W5_Biotechnology 8_ Outline the main steps in recombinant DNA Technology

Introduction:

Recombinant DNA technology or genetic engineering is a method that allows scientists to study, improve, or manipulate the genetic material of a cell to create
genes of interest or transgenes. The technology allows the transfer of a specific gene from different organisms to produce a new trait in an organism. The DNA
combined from different cells is called recombinant DNA and the organisms produced by the technology are called GMO’s or transgenic organisms. Genetic
engineers can now employ the molecular tools and techniques that allow manipulation of DNA sequences in the laboratory to identify genes that control a
desired trait, extract them, copy them, and insert them into another organism.

ACTIVITY 1 Genetic Engineering of Microorganisms

Direction: The steps involved in the process of genetic engineering of microorganism are
shown in the figure below to illustrate the introduction of the human insulin
gene into bacteria. Analyze and interpret the figure below and answer the
following questions:

1. What are plasmids and tell how they are used in genetic

engineering? _______________

2. What is the function of restriction enzyme?

____________________________________

3. What is the role of bacterial cell in genetic engineering? __________________________

4. Read different reference materials to gain more information about recombinant DNA

technology. From your readings, supply the missing word to complete the basic steps

involved in genetic engineering of microorganisms.

Step 1. The _____ of interest which is human insulin, is isolated from the human DNA
and cloned. The _____ of DNA means making multiple exact copies of DNA or
gene.

Step 2. The human insulin gene is inserted into a _____, a plasmid at specific sites cut by

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 an enzyme called restriction enzyme. A plasmid which can carry a DNA fragment
contains the promoter elements and terminator sequence that will work for the
bacterial cells.

Step 3. The recombinant plasmid is introduced into bacterial cell by physical means. The
_____ are subjected to heat and then put an ice and this will result into
formation of pores in the cell membrane that allow the DNA to get inside the cell.
This process is called transfection or transformation. The _____ plasmid is
multiplied into many copies in the cell.

Step 4. This process results in the production of many copies of the inserted human insulin
gene. This is called _____ of the human insulin gene by bacterial cells.

ACTIVITY 2 Be A Plant Genetic Engineer!

Direction: Select the steps involved in the genetic engineering of corn with the Bt gene to
produce insect-protected corn in the proper order.

Genetic Engineering Maize to Produce Bt Corn

1. 2. 3. 4. 5.

ACTIVITY 3 Welcome to the World of Transgenic Organisms

Direction: Transgenic organisms are organisms that have been transformed by the insertion of one or more foreign gene(s) obtained from another organisms.
“Foreign” means the donor organisms belong to different species. Here are some of the genetically modified organisms, also called transgenic organisms.
Write the peculiar traits they acquired through recombinant DNA technology.

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 Tomato with Antarctic fish antifreeze gene

____________________________________________________________________________________

____________

Supersalmon with growth genes

____________________________________________________________________________________

____________

Bt Talong
Bt Corn

____________________________________________________________________________________
____________________________________________________________________________________

____________
____________
GM Bacteria through the insertion of a human insulin gene

____________________________________________________________________________________

GM Pigs with human genes ____________

____________________________________________________________________________________

Glow in the dark mice ____________

____________________________________________________________________________________

____________ Golden Rice

____________________________________________________________________________________

____________

Glow in the dark tobacco plant

____________________________________________________________________________________

____________

ACTIVITY 4 Find TEN or more words related to Recombinant DNA technology

in the grid below. Look up, down, forward, backward, and diagonally.

T R A N S F O R M A T O N O E N E G

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 R A B C D E F G H I C J K L M M N E

A O P Q R S T U V W U X Y Z O A B N

N O I T C E F S N A R T X N Y Z X E

S C D E F G H I J K T L E M N O P T

G Q R S T U V W X Y S G Z A B C D I

E E F H Y B R I D D N A G H I J K C

N L M N O P V Q I S O L A T I O N E

I R S T U E X V M W C X Y Z A B C N

C D E F C G Y H S I E Y J K L M N G

O L P T X Q R S A T N Z U V W X Y I

S T O U Y V W X L R E T O M O R P N

S R T N Z U V W P X G Y Z A B C D E

E F G H I J K L M N O P Q R T U V E

W X Y R A N D T N A N I B M O C E R

Z A B C D E G E N E G U N F G H I I

R E S T R I C T I O N E N Z Y M E N

X Y Z J K L M R O T A N I M R E T G

What new words did you come across in this activity? ______________________________

_________________________________________________________________________

REFERENCES:

Printed Books

th
Herren, Ray V. (2015). Introduction to Biotechnology. 4 edition CENGAGE
Learning Asia Pte Ltd
Tecson-Mendoza, E.M. (Ed) Issues and Concerns of Modern Biotechnology. NAST. Philippine Science Heritage Foundation Inc., Manila.

nd
Tecson-Mendoza, E.M. Understanding Biotechnology. 2 edition
NAST. Philippine Science Heritage Foundation Inc., Manila

Online References

HYPERLINK "http://www.bio.org" http://www.bio.org

HYPERLINK "http://www.isaaa.org" www.isaaa.org
HYPERLINK "http://www.aphis.usda.gov" http://www.aphis.usda.gov

MELC: Quarter 3_Week 5_Biotechnology 8_ Outline the main steps in Recombinant DNA Technology Prepared by: CORAZON V. WONG / Ramon

Magsaysay Cubao High School