27/2/23, 9:22 PhysioEx Exercise 1 Activity 1

PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability
Activity 1: Simulating Dialysis (Simple Diffusion)
Name: María Fernanda Reyes López
Date: 27 February 2023
Session ID: session-d51f369b-78b6-eb6a-cc3f-307809a5b32f

Pre-lab Quiz Results

You have not completed the Pre-lab Quiz.

Experiment Results
Predict Questions
1 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse
through the 20 MWCO membrane?

You did not answer this question.

2 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607
amino acids, and the average molecular weight of a single amino acid is 135 g/mol.

Which of the following will be able to diffuse through the 200 MWCO membrane?

Your answer: glucose only.

Stop & Think Questions

1 The reason sodium chloride didn't diffuse left to right is that

You did not answer this question.

Correct answer: the membrane pore size was too small.

2 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average
molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes
at the 20 MWCO because

You did not answer this question.

Correct answer: glucose and albumin are both too large to pass.

3 The rate of diffusion for urea

You did not answer this question.

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Correct answer: is slower than that for sodium because urea is a larger molecule.

Experiment Data
Solute MWCO Left Solute (mM) Right Solute Average
(mM) Diffusion
(mM/sec)
Na+ Cl- 20 9.00 0.00 0.0000
Urea 20 0.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na+ Cl- 20 0.00 0.00 0.0000
Urea 20 9.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na+ Cl- 50 9.00 0.00 0.0150
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na+ Cl- 50 18.00 0.00 0.0273
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na+ Cl- 100 9.00 0.00 0.0150
Urea 100 0.00 0.00 0.0000
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na+ Cl- 100 0.00 0.00 0.0000
Urea 100 9.00 0.00 0.0094
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na+ Cl- 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 0.00 0.00 0.0000
Glucose 200 9.00 0.00 0.0042
Na+ Cl- 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 9.00 0.00 0.0000
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Glucose 200 0.00 0.00 0.0000

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the

left beaker was to

You correctly answered: increase the rate of diffusion.

2 Describe the difference between the rate of diffusion seen for sodium and urea.

You correctly answered: Urea diffused more slowly because it is larger than sodium.

3 Which of the following solutes did not pass through any of the membranes?

You correctly answered: albumin.

4 When diffusion stops, we say the solution has reached

You correctly answered: equilibrium.

Review Sheet Results

1 Describe two variables that affect the rate of diffusion.

Your answer:

Las dos variables que afectan a la difusión es la agitación y el gradiente de difusión.

2 Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How
well did the results compare with your prediction?

Your answer:

Debido a que el peso de la urea es grande y la membrana pequeña.

3 Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO
membrane. How well did the results compare with your prediction?

Your answer:

solo glucosa.

4 Put the following in order from smallest to largest molecular weight: glucose, sodium
chloride, albumin, and urea.

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27/2/23, 9:22 PhysioEx Exercise 1 Activity 1

Your answer:

Cloruro de sodio, urea, glucosa y albúmina.

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