NSS Biology /Test / Chapter 22/P.

NSS Biology Test (Chapter 22: Respiration)

Section A: Multiple Choice (8 marks)
1. The graph below shows the rate of oxygen uptake due to respiration of isolated barley
embryos cultured in solutions containing 4% of glucose and different concentrations of
phosphate.

Which of the following factors most likely

limits the rate of respiration between B
and C?
A ATP
B ADP
C oxygen
D phosphate

2. During a marathon, aerobic respiration predominates over anaerobic respiration because

A more power is produced using aerobic respiration than anaerobic respiration.
B fat stores are consumed during the first few minutes of exercise.
C aerobic respiration uses glucose very efficiently compared to anaerobic respiration.
D anaerobic respiration produces lactic acid.
3. The cell organelle shown below is magnified ten thousand times.

What is the actual size of the organelle?

A 0.04 μm B 0.4 μm C 4 μm D 40 μm
4. Which of the following processes consume(s) energy from ATP?
(1) breakdown of glucose to triose phosphate in glycolysis
(2) regeneration of 4-C compound in the Krebs cycle
(3) regeneration of 5-C compound in the Calvin cycle
A (1) only B (2) only C (1) and (2) only D (1) and (3) only
5 Which of the following substances is/are produced when our skeletal muscle cells respire
anaerobically?
A lactic acid B ethanol
C lactic acid and carbon dioxide D ethanol and carbon dioxide
6. Which of the following is the importance of anaerobic respiration during exercise?
 NSS Biology /Test / Chapter 22/P.2

(1) It allows higher contraction rates of muscles.

(2) It produces lactic acid.
(3) It provides additional energy.
A (1) only B (1) and (3) only C (2) and (3) only D (1), (2) and (3)

7. The graph below shows the amount of oxygen a person breathed in before,during and
after doing exercise.

Which area in the graph represents oxygen debt?

A I B II C III D IV

8. Which of the following molecules contains the largest amount of chemical energy?
A ATP B ethanol C lactic acid D glucose

Section B: Questions (22 marks)

1. The diagram below shows an experiment, which was carried out to investigate aerobic
respiration of a grasshopper.

a What is the function of potassium hydroxide? (1 mark)

b Before the experiment started, the clip was opened for a while and then closed.
Why was this necessary? (2 marks)
 NSS Biology /Test / Chapter 22/P.3

c Explain the change in coloured liquid level in arms X and Y after two hours.(5 marks)

2. The diagram below shows glycolysis and the Krebs cycle in aerobic respiration.

a Name compound X. (1 mark)

b Where do glycolysis and the Krebs cycle occur precisely in a cell? (2 marks)
c i Indicate on the diagram by writing a letter ‘U’, where ATP is used in
respiration. (1 mark)
ii Indicate on the diagram by writing a letter ‘P’, where ATP is produced during
respiration. (2 marks)
d Describe the fate of the NADH and FADH produced. (3 marks)
e Describe what happens to pyruvate in anaerobic conditions in muscle cells.(2 marks)
f ATP is also produced in anaerobic conditions but the yield is much less than that in
aerobic respiration. Explain why. (3 marks)
 NSS Biology /Test / Chapter 22/P.4

NSS Biology Test (Chapter 22: Respiration) – Answer Sheet

Name: F.5 ( )
Section A: Multiple Choice (8 marks)
1. 2. 3. 4. 5. 6. 7. 8.

Section B: Questions (22 marks)

1a

2a

b
 NSS Biology /Test / Chapter 22/P.5

c (i),(ii)

f
 NSS Biology /Test / Chapter 22/P.6

NSS Biology Test (Chapter 22: Respiration) – Suggested Answer

Section A: Multiple Choice (8 marks)
1. 2. 3. 4. 5. 6. 7. 8.
B C C D A B C D
Section B: Questions (22 marks)
1(a) Potassium hydroxide is used to absorb carbon dioxide in the test tube. (1)
(b) It was to equalize the pressure on both sides of the U-shaped capillary (1)
tube.
This ensured that the liquid levels in the two arms of the capillary tube (1)
were the same before the experiment started.
(c) The grasshopper carried out aerobic respiration and took in oxygen in (1)
the test tube.
Carbon dioxide given out during respiration was absorbed by potassium (1)
hydroxide.
As the amount of gas inside the test tube decreased, the pressure (1)
dropped.
The atmospheric pressure was greater than the pressure inside the tube. (1)
Therefore the liquid level in arm X rose and the liquid level in arm Y (1)
fell.
2(a) Carbon dioxide (1)
(b) Glycolysis occurs in the cytoplasm. (1)
The Krebs cycle occurs in the mitochondrial matrix. (1)
(c) i Between glucose and triose phosphate (1)
ii Between triose phosphate and pyruvate (1)
Between 6-C compound and 4-C compound (1)
(d) NADH and FADH lose hydrogen to hydrogen carriers and the hydrogen (1)
passes from one carrier to another.
Energy is released during the process and ATP is formed. (1)
NAD and FAD are regenerated. (1)
(e) Pyruvate accepts hydrogen from NADH. (1)
Pyruvate is reduced to lactic acid. (1)
(f) In anaerobic conditions, there is no oxygen which acts as the final (1)
acceptor of hydrogen.
The cell cannot regenerate NAD and FAD, thus the Krebs cycle and (1)
oxidative phosphorylation cannot operate.
Only glycolysis occurs, thus only 2 ATP are produced. (1)