Lab # 9

Determination of pulse
rate recovery after physical
exercise in response to

1st year- Physiology Lab

autonomic control

Muhammad Amir Mustufa

M.Sc., M.Phil (Neurophysiology)
Lecturer- Physiology- UQU
BLS Instructor – UQU
Electro-neurophysiologist 1
 learning objectives 2

By the end of the practical students should be

able to:

 Identify Radial, Carotid, and brachial pulses in a

subject.
 Record Radial pulse in resting state and after physical
exercise.
 Describe division of nervous system.
 Describe origin, types and functions of the autonomic
nervous system.
 List differences between sympathetic and
parasympathetic nervous system.
1st year- Physiology Lab 3/27/2021
 3
Practical Outline

• Composition of Nervous system

• Differences between sympathetic

and parasympathetic nervous
system.

• Regulatory effects of autonomic

nervous system.

• Procedure of radial pulse recording.

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 Introduction 4

YA we m
Nervous system regulates body activities rapidly
f through nerve
Miimpulses (action potentials). own
Nervous system is divided into two main parts:
 A. Central Nervous System (CNS)
É
0
É IT
Brain and spinal cord.

15
 B. Peripheral Nervous System (PNS)

22
Composed of all nerves coming out or into the CNS.
These include spinal and cranial nerves. (Somatic &
autonomic nervous system)

35
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1 NS

CNS PNS

Somatic
Spinal ANS
NS a
Brain (sensory&
Cord (sensory&
a motor) motor)
3

Symp.NS Paras.NS

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J 3
s
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PNS Division

Peripheral
NS
I
I Sensory & motor
neurons

Somatic NS – Autonomic NS --
Voluntary Involuntary
Foot
Input from sense organ Input from internal
receptor
Output to skeletal As I
Output to heart, smooth
muscles e
muscles and glands

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 7
ANS Division
It
Autonomic nervous
system
N Involuntary
E Input from internal receptor
E
Output to heart, smooth muscles &
glands

4
Sympathetic Motor Parasympathetic Motor
system System

IE
‘Fight or flight’ responses
Neurotransmitter : noradrenaline
IE Relaxing response
Neuroransmitter : acytylcholine

Bo
‘Adrenergic System’ ‘Cholinergic System’

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 OF
8
g

I
s By

I pp t

t
I
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 9

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Comparison of the effect of sympatheetic 10
and parasympathetic nervous system on
different systems & glands

In d

É
1
of a

it

I
3
D
A 27/03/2021
 Recording radial & carotid pulse 11
is I
t Radial
autry
tas
ji
I
I

9
carotid
autry

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Procedure
 The subject should be in a resting state
(physically and mentally) before recording
the pulse. Make the subject to lie down/sit
down comfortably at the examination
table/chair for some time.
 Place fingertips (ring, middle and index
finger) over the radial artery in the
ventrolateral region near the subject's wrist
joint.
 Feel and count the number of pulses for
30Sec. and note them down by multiplying
with 2 to convert it into the number of heart
beats per minute. 27/03/2021
 Procedure 13

 Repeat the pulse counting twice and note down the

reading in pulses per min.
 Calculate the average of these three values and
note down in the table.
 Repeat the whole above procedure after proper
physical exercise. For exercise purposes, run and
take three to five rounds in the lab corridor or if
treadmill is available do exercise at least 2-3
minutes.
 Measure the radial pulse and record your
observation in the given table.
 Note the time of the radial pulse come to the
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normal resting state after exercise.
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Observation
Observed values of pulse rate obtained before and after exercise
After exercise (one observation only)
Before exercise
obs.
repeat 3 times Immediately 1 min 2 min 3 min 4 min 5 min
PAIN I
60 90 B
1

2
7
31T yo yo yo 100
85
3 150
67
Average É
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 Parasympathetic
Sympathetic NS 15
NS

E
E
o
3
s
f j

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Identify the artery used in above picture for pulse

recording: a
A. Brachail artery.
B. Carotid artery.
C. Radial artery. s
D. Femoral artery.
E. Popliteal artery.
Radio's
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O
Parasympathetic nervous system increases
the activity of:
 A. Cardiovascular system.
 B. Digestive system. EEK
range
 C. Respiratory system.
 D.
 E.
Sweat glands.
Piloerector muscles
15
É
X 2
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 References 18

 Guyton Text book of medical Physiology 13th

edition.
 Hutchisons clinical methods 20th edition.

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thank you

1st year- Physiology Lab 3/27/2021