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    Crisper Design

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    kuskucum
    This document describes a vector for expressing a guide RNA and Cas9 for CRISPR genome editing. The vector contains an araBAD promoter upstream of the Cas9 gene and a separate promoter driving expression of a guide RNA. Unique restriction sites flank different components to allow modular assembly of the various DNA parts.

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    Crisper Design

    Uploaded by

    kuskucum
    5 views1 page
    This document describes a vector for expressing a guide RNA and Cas9 for CRISPR genome editing. The vector contains an araBAD promoter upstream of the Cas9 gene and a separate promoter driving expression of a guide RNA. Unique restriction sites flank different components to allow modular assembly of the various DNA parts.

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    CrisperDesign

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    This document describes a vector for expressing a guide RNA and Cas9 for CRISPR genome editing. The vector contains an araBAD promoter upstream of the Cas9 gene and a separate promoter driving expression of a guide RNA. Unique restriction sites flank different components to allow modular assembly of the various DNA parts.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    5 views1 page

    Crisper Design

    Uploaded by

    kuskucum
    This document describes a vector for expressing a guide RNA and Cas9 for CRISPR genome editing. The vector contains an araBAD promoter upstream of the Cas9 gene and a separate promoter driving expression of a guide RNA. Unique restriction sites flank different components to allow modular assembly of the various DNA parts.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    ori araBAD

    Promoter Gama Beta Exo L3


    Term Cas9 araBAD
    Promoter

    XbaI

    gRNA
    DWN HOM UP HOM zcnR araC

    N20_RC
    N20
    gRNA_Vector_R gRNA_F gRNA_R CRISPR_Vector_R

    AhdI XbaI
    XbaI

    BsaI
    AarI

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