Leica BOND‑MAX

Fully automated IHC & ISH

Quality. Efficiency. Agility.
 3

Difficult questions require accurate

and precise answers.
When a patient is waiting anxiously, fully automated immunohistochemistry (IHC) and
in situ hybridization (F IS H&C IS H) helps with quality staining for timely results and diagnostic
confidence. It’s how modern histology laboratories deliver better patient care.

›QUALITY
Increase diagnostic confidence

›EFFICIENCY
Help more patients and lower costs

›AGILITY
Quickly adapt to new challenges and opportunities

CONNECT SELECT REFINE

Reduce work and potential errors.
Leica BOND-MAX connects to common
LIS systems and the Leica CEREBRO
sample tracking system as well as an
unlimited number of BOND instruments.
› Leica BOND LIS-ip
› Leica CEREBRO
Choose the ideal reagents.
Access the full range of exceptional
Novocastra antibodies and probes or
TM
select third-party antibodies.
› Novocastra BOND RTU reagents
› Leica BOND Polymer Refine Detection
› Open primaries
Unrivalled service and support.
Leica Biosystems’ IHC & ISH scientific
experts help with applications
knowledge, LEAN workflow analysis,
preventative maintenance and
instrument support.
› Applications advice
› LEAN support
› Flexible maintenance contracts

Human gastrointestinal tract. Immunohistochemical

staining of gastrointestinal stromal tumor (GIST) for
DOG-1. Stained using Novocastra DOG-1 for BOND
(PA0219, Clone K9) with BOND Polymer Refine
Detection ™ (DS9800).
4

Quality

Leica BOND-MAX helps you create diagnostic confidence. With full automation
reducing repeats and standardizing staining, pathologists receive high-quality slides
that help ensure patients get accurate answers.

TOTAL TISSUE CARE CLARITY AND ACCURACY COMPLETE CONSISTENCY

The BOND Covertile™ system protects
patient tissue with very gentle reagent
application and incubation protection
to help you minimize tissue damage
and repeats.
Deliver high-quality staining with BOND
reagents. These pre-optimized reagents
deliver exceptional automated IHC & ISH
performance that consistently scores
highly on EQA schemes throughout the
world.
Leica BOND-MAX provides complete
on-board IHC & ISH automation that
removes process variation to help you
deliver the consistent staining
pathologists expect.

Left. Human lung. Immunohistochemical staining of

malignant pulmonary carcinoma cells for thyroid
transcription factor 1. Stained using Novocastra Thyroid
Transcription Factor-1 for BOND (PA0364, Clone SPT24)
with BOND Polymer Refine Detection ™ (DS9800).
Right. Human kidney cortex. Immunohistochemical
staining of proximal tubular brush border. Stained using
Novocastra Renal Cell Carcinoma Marker (NCL-RCC, Clone
66.4.C2) with BOND Polymer Refine Detection ™ (DS9800).
Increase diagnostic
confidence.
6

Efficiency

Increase efficiency, eliminate bottlenecks and reduce backlogs. Leica

BOND-MAX has the flexibility and organization to promote Lean workflows
so your laboratory can stain more slides in less time.

EASY TO RUN CONTINUOUS ALWAYS READY

Keep your work flowing by eliminating
complexity. Leica BOND-MAX runs are
quickly configured and ready-to-use
reagents reduce preparation work to
simply scan, load, press play and walk
away.
With three independent slide trays, each
Leica BOND-MAX is effectively three
stainers in one instrument. So you have
the flexibility of continuous processing
while keeping your slides together to
reduce sorting.
Quickly process urgent slides. Continuous
processing means a spare tray is never
far away, while ready-to-use reagents
eliminate preparation work – just load
and run.

Human breast. Immunohistochemical staining of carcinoma

for estrogen receptor. Stained using Novocastra Estrogen
Receptor for BOND (PA0151, Clone 6F11) with BOND
Polymer Refine Detection ™ (DS9800).
Help more patients and
lower costs.
8

Agility

Quickly adapt to new opportunities. It’s easy to introduce Leica BOND-MAX

into any laboratory environment. With a small foot print, lower costs and
widespread industry acceptance, Leica BOND-MAX helps you react quickly
when you have new opportunities or challenges.

COMPACT SIZE EFFICIENT RESOURCE ALLOCATION READY NOW

With a small footprint and benchtop
mounting, Leica BOND-MAX will fit
easily into most existing laboratory
layouts to negate the need for extensive
remodeling or large areas of dead space.
You don’t need a large throughput to Leica BOND-MAX is a proven industry
justify high-quality, fully automated performer. The availability of trained
IHC & ISH. By focusing on quality and operators plus Leica support and
efficiency, while eliminating functionality ready-to-use reagents means even a
you don’t need, Leica BOND-MAX frees laboratory new to fully automated
financial resources. IHC & ISH can consistently deliver
high-quality staining.

Human skin. Immunohistochemical staining of

malignant melanoma cells for melanoma marker.
Stained using Novocastra HMB45 (Melanoma
Marker) for BOND (PA0027, Clone HMB45) with
BOND Polymer Refine Detection ™ (DS9800).
Quickly adapt to new
challenges and
opportunities.
10

System Specifications

QUALITY EFFICIENCY AGILITY

Covertile technology Optimized BOND Continuous processing Leica BOND control Small footprint
reagents center
Unlike other systems, A full range of Leica BOND-MAX is A single source for With benchtop mounting
Leica BOND instruments Novocastra BOND effectively three operation and reporting and a small footprint,
are gentle on the tissue reagents provides the independent stainers in for an unlimited number Leica BOND-MAX easily
and ensure total tissue ideal match of one instrument – the of Leica BOND fits into tight laboratory
care with exceptionally consistent quality and ideal balance of instruments plus LIS spaces.
low dispense and waste effortless workflow. flexibility and and sample tracking
volumes. organization. connectivity.

SYSTEM SPECIFICATIONS

Dimensions 760 mm (W) x 703 mm (H) x 775 mm (D) Mains frequency 50/60 Hz
29.9 in (W) x 27.6 in (H) x 30.5 in (D)
Power consumption 1000 VA
Weight (dry) 120 kg
Maximum operating temperature 35 °C
265 lb.
95 °F
Slide capacity 30
Minimum operating temperature 5 °C
Finished trays (10 slides) may be
41 °F
replaced continuously
Temperature required to meet 18–26 °C
Reagent container capacity 7 mL and 30 mL
staining performance 64 –79 °F
Number of reagent containers 36 requirements

Bulk reagent container capacity Operating humidity 10 to 80% RH

1 L or 2 L
(noncondensing)
Hazardous waste container 2L
Operating altitude 0 to 1600 m (5250 ft.) above sea level
capacity

External Bulk waste container Sound pressure level output < 85 dBA maximum
9L
capacity (at 1 m) < 65 dBA normal operation

Operating voltage 103.4 V~ to 127.2 V~

(for nominal voltage 110 V~ to 120 V~)
or
206.8 V~ to 254 V~
(for nominal voltage 220 V~ to 240 V~)
www.LeicaBiosystems.com

TOTAL HISTOLOGY SOLUTIONS

High-quality IHC and ISH staining depends upon expert tissue preparation
and sectioning. With a broad range of histology instruments, consumables
and applications knowledge, Leica Biosystems offers research-specific
histology solutions that will help you efficiently prepare high-quality
sections for advanced staining.

· Tissue preparation
· Sample identification and tracking
· Tissue processors, cassettes and paraffin wax
· Embedding centers, accessories and paraffin wax
· Microtomes, blades and slides
· Coverslippers, mountants and coverslips
· Microscopy and imaging solutions
· Expert service and support
· Ongoing maintenance support

LEICA BIOSYSTEMS
Leica Biosystems is a global leader in workflow solutions bringing
Leica Biosystems brings
histopathology laboratories and researchers the highest quality, most together products, quality and
comprehensive product range in anatomical pathology. With complete support. Offering a complete
histology systems featuring innovative automation, NovocastraTM solution that helps you advance
reagents and Surgipath® consumables, Leica Biosystems offers the ideal
workflows, enhance diagnostic clarity and
product for each histology step and high-productivity workflow solutions
for the entire laboratory. deliver what really matters – better patient care.

Leica Biosystems – an international company with a strong network of

worldwide customer services:

North America Sales and Customer Support Scan this tag and use your smart phone
North America 800 248 0123
to find out more about Leica Biosystems
Asia/Pacific Sales and Customer Support
Australia 1800 625 286 Total IHC and ISH Solutions.
China +85 2 2564 6699
Japan +81 3 5421 2804
South Korea +82 2 514 65 43
New Zealand 0800 400 589
Singapore +65 6779 7823
Europe Sales and Customer Support 95.10629 Rev B - Order no. 1195.10629 · 09/2012 · Copyright © by Leica Biosystems
For detailed contact information about European sales offices or distributors please Melbourne Pty Ltd, Melbourne, Australia, 2012. Subject to modifications. LEICA and the
visit our website.
Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
Delivering What Really Matters

Bond-III Fully Automated IHC and ISH

TM

Total Performance for Faster, Better, More Efficient Slide Staining


UP TO 50% FASTER
Three additional robots for
bulk fluid dispensing provide
stained slides to pathologists
more quickly.
2 3
CONTINUOUS PROCESSING
Three independent trays of
10 slides each allow continuous
parallel processing.
Bond-III
TM
S P E E D really matters
With class-leading speed for all routine IHC and ISH tasks, including the ability to stain
more slides in less time, Bond-III provides critically important advantages that benefit
histologists, managers and pathologists. That’s the power of Leica Total Histology™ —
delivering a full range of histology systems that really matter.
SIMULTANEOUS DETECTION FASTER IS BETTER
Space for 36 reagents on Bond-III’s superior speed
four trays provides the flexibility significantly improves productivity,
to perform IHC and ISH with reduces turnaround time and, most
multiple detection systems importantly, helps you deliver
at the same time. better patient care.

LEAN HISTOLOGY™ WORKFLOWS
Designed to optimize Lean
Histology workflows, Bond-III
increases uptime and reduces
waste, while also providing
LIS connectivity, eliminating
the need for data entry.
4 5
EASY CONTAINER MANAGEMENT
High-capacity containers require
less reagent and waste management,
and can be filled without removal
from the instrument.
Bond-III
TM
E F F I C I E N C Y really matters
With Bond-III, less is more, including less reagent use and hazardous waste than any
other fully automated system. This delivers less maintenance, waste disposal costs and
environmental impact. A full range of high-efficiency management features means less
staff intervention, providing more time for what matters most.
LESS REAGENT MANAGEMENT STREAMLINED MADE SIMPLE
Real-time fluid status allows more Enhanced, easy-to-use software
efficient reagent management. interface requires minimal staff time to
learn and manage, and flexible
modularity enables seamless addition
of any member of the Bond family for
expanded capacity as your lab grows.

COVERTILE™ TECHNOLOGY
Leica Microsystems’ patented
Covertile ensures gentle tissue care
and low volume, uniform reagent
coverage throughout the staining
process, preserving tissue integrity
and minimizing repeats.
6 7
STAINING REAGENTS
A full range of Bond
ready-to-use antibodies
designed with Novocastra™
Science is preoptimized for
Bond to ensure consistently
high-quality staining.
Bond-III
TM
Q U A L I T Y really matters
At Leica Microsystems, quality is at the heart of Total Histology.™ With three generations
of superior Bond staining consistency, Bond-III assures delivery of clear and accurate
staining for unsurpassed diagnostic confidence. Quality matters to us because it matters
to you, and your patients.
COMPACT POLYMER™ DETECTION QUALITY THAT’S CUSTOMIZED
High concentration of enzyme The flexibility within our advanced
for each antibody assures software allows simple customization
stains are crisp and well-defined, and control of staining quality,
providing highly sensitive matching the protocol to the needs
detection and specificity without of your lab and your pathologists.
background staining.
 Bond-III
TM

SYSTEM SPECIFICATIONS

Processing module dimensions 775 mm x 785 mm x 1,359 mm (30.51 in x 30.91 in x 53.50 in)

© Leica Microsystems GmbH • HRB 5187 • 09/2009 • 95.8385 Rev B

Weight (dry) 246 kg (243 lb)
Processing temperature Ambient to 100°C
Slide capacity 3 independent trays of 10 slides 30 slides total capacity
Reagent container capacity 7 mL or 30 mL
Number of reagent containers 36 (4 trays of 9)
Dispense volume 150 µL
TM

Modularity 5 processing modules per controlling PC. Any combination of Bond-x ,

TM

Bond-max and Bond-III

Waste disposal Separation of hazardous and nonhazardous waste
System mounting Freestanding
Database Full tracking and reporting of all slides
Full tracking and traceability of reagent lot numbers, expiration dates and usage
Laboratory information system (LIS) integration Optional upgrade package, compatible with HL7 v2.4/2.5
and LIS02-A2 (ASTM 1394)

SYSTEM COMPARISON Bond-max Bond-III

High-Quality Staining ••• •••

Efficiency •• •••

Ease of Use •• •••

Speed of Processing •• •••

Leica Microsystems – an international company with

a strong network of customer services
The Bond system is available through the following offices:

North America
Sales orders/Customer support 800 248 0123

Asia/Pacific
Sales orders/Customer support Aus 1800 246 797
Sales orders/Customer support NZ 0800 400 589

Europe/Middle East/Africa
Sales orders/Customer support UK 0800 298 2344
Sales orders/Customer support ROI 1800 242 612
Sales orders/Customer support EU +44 1908 246 246

www.leica-microsystems.com