Trophozoite (Iron

hematoxylin SHAPE SIZE MOTILITY STRUCTURE FLAGELLA

preparation)
Giardia lamblia bilaterally
9.5-21
symmetrical, Cytoplasm- grayish violet, fairly granular ;
micrometre in
pear-shaped, slow, sucking disk @ ventral anterior ; 2 4 pairs : lateral crossed, lateral
length, 5-15
rounded tumbling, like nucleus, ovoid shaped @ the anterior uncrossed, central and posterior
micrometre, 2-4
anteriorly, a falling leaf portion with a central darkly stained flagella
micrometre in
pointed karyosome ; parallel axonemes
thickness
posteriorly
Chilomastix mesnili
Cytoplasm- grayish violet , spherical
6-20 micrometre nucleus is located medially @ anterior
Asymmetrical, in length, 3-10 Stiff and portion with small karyosome ; cytostome
3 anterior flagella
pear shaped micrometre in rotary @ one side of the nucleus & is rounded
width anteriorly and posteriorly ; spiral groove
runs obliquely along the ventral surface

Trichomonas 5-14 micrometre Cytoplasm-grayish violet, finely granular;

hominis in length anterior ovoidal nucleus with central
Lashing flagella; 3-5 anterior
(average: 7-8 karyosome; anterior cytostomal cleft;
Pyriform Rapid jerks flagella and one@ undulating
micrometre) , 7- undulating membrane is located @ the
membrane
10 micrometre in opposite side of cytostome; anterior
width protruding axostyle;
Trichomonas Cytoplasm-grayish violet, granular (
7-23 micrometre
vaginalis siderophil granules); anterior ovoidal 4 anterior flagella and 5th flagella
in length Cork screw,
Pyriform nucleus; anterior cytostome ; short along the margin of undulating
(average 13 slow
undulating membrane; extended membrane
micrometre)
posterior axostyle
Dientamoeba Granular cytoplasm with vacuoles
fragilis Actively
containing bacteria ; 2 nuclei with Flagella extends from the cell wall
Irregular 3-22 micrometre motile;
karyosome consisting of 4-8 chromatin like pseudopods
progressive
granules

Trichomonas hominis ,Trichomonas vaginalis Dientamoeba fragilis = all three have NO CYST FORM
Cyst (iron hematoxylin prep.) SHAPE SIZE STRUCTURE
Double wall; 1-4 spherical nuclei with central dark violet
8-12 micrometre, 7-10
Giardia lamblia Ovoidal, football-shaped karyosome; deep violet, crossed, parallel fibrils ; grayish violet,
micrometre in width
granular cytoplasm
Lemon-shaped, nipple like
Double wall ; granular and grayish violet cytoplasm and is clearly
Chilomastix mesnili protruberance @ anterior 7-10 micrometre
separated from wall, ; 1-2 nuclei
portion
Cyst (Normal Saline Soln.) STRUCTURE
Giardia lamblia Greenish cytoplasm; hollow, black dot nuclei; black crossed fibrils
Chilomastix mesnili Cytoplasm is colorless; nucleus as black dot (rarely seen)
Cyst (Iodine Soln.) STRUCTURE
Giardia lamblia Yellow cytoplasm; hollow, black dot nuclei; browncrossed fibrils
Chilomastix mesnili Yellow cytoplasm ; nucleus and cytostome ay be seen

Dientamoeba fragilis

Iron hematoxylin stain

 AMASTIGOTE PROMASTIGOTE EPIMASTIGOTE TRYPOMASTIGOTE
(Leishmania) (Leptomonas) (Crithidia) (Trypanosoma)
Intracellular in
In midgut, later
macrophages
in proboscis of
L. of skin & may
sand fly, transfer Lacking Lacking
braziliensis be carried to
stage to man
mucocutaneous
Culture
junctions
Intracellular in
In midgut, later
macrophages,
in proboscis of
predominantly
L.donovani sand fly, transfer Lacking Lacking
in liver, spleen,
stage to man
bone marrow &
Culture
lymph nodes
Intracellular in In midgut, later
macrophages in proboscis of
L. tropica of skin & sand fly, transfer Lacking Lacking
subcutaneous stage to man
tissue Culture

AMASTIGOTE PROMASTIGOTE EPIMASTIGOTE TRYPOMASTIGOTE

(Leishmania) (Leptomonas) (Crithidia) (Trypanosoma)
Intracellular in
macrophages
esp. in skin, In feces of triatomid
liver, smooth In mid gut of bug, transfer stage
Transitional
T. cruzi muscles with triatomid bug to man, present in
stage only
affinity for Culture blood stream only
cardiac during acute attacks
muscle
Cell cultures
In proboscis of
In salivary tsetse fly, transfer
st
glands & gut of stage to man, 1 in
T.gambiense Lacking Lacking
Tsetse fly blood stream then
Culture in lymph node, later
in CNS
In proboscis of
In salivary tsetse fly, transfer
glands & gut of st
T.rhodesiense Lacking Lacking stage to man, 1 in
Tsetse fly
Culture blood stream then
in lymph node