Professional Documents
Culture Documents
Botany
Botany
"'ci"ia'ii,iiu
5.
V v:Do,,=t. ,t ' tv er rr- ercr r' *ttw v''r ,., ru, rFl? .tr44r sctrvent and traveled the farthest up tire
c
stn;--r
of paper? Why?
phase?
:9SNT-' the tast up the stationary slowly while others -anro ffin\/A clnrvlvffi6 .th"* traveled very fast h.Fracrrf an\t./.r<gf,trotl9 INP SOt
lx litq
-. -
"
7. @lnotnatograp",
" - lT ls
rJsr '2 ,p
'<r^' i*tn'tf
,L+
Indicator of Photosynthesis
the form of simpie
sr.rgar phosphates.
Photosyntiresis allovrs piant-s tc chlorophyll is an essential factor in photosynthesis' organic moiecules :;e
i'
These
LU einer
a.
Psrls'
..
pl*t
pa.fts
storage organs-such as roots and of the leaves. fn.y may also be detlcted in prirnary an enlarged stem modified for food storage contains stemS. The potato tuber, which is
strLrch'
b.utilizedasenergysourcetluoughcellularrespiratton'or use of the plant' c. converted into storage.o*po*id,like starch for future the- detection of starch in activity in plants is Hence, one indication of prt"t"rviirrriic in actively photcsynthesizing grncrl organs. The presen." o1starcir may be detected
durnbcane or kutsarita' test tube' test MAT&RIALS: , varip g?tadleave-s of Polyscias, bath, ta'p \'vater, 95% ethyl alcohol' beaker' raok, test tube holder, petri dishes, water
tube
PROCEDURE:
I l.
UUI rne iear Lq !;Hlraiati' Get a healthy variegated leaf of Poiyscias, ciumbcane or l(utsarlta. and white portions of the white portions from the green ones. Note the shape of the green into a test tube % the leaf. Boil the leaf biades in water for 15 to 20 minutes. Transfer gs % ethyl alcohol. Boil in a water bath until ihe leaves are completely
a petri dish' Use the iodine test decolorized. Rinse the leaf blades in tap urate:.. Piace in for the presence of starch. Note the color changes in the leaf' part of the potato tuber. Stain wi;lt z. prepare a fresh mount using a thin section of the inner thin slice of the potato' iodopotassium iodide; allow the stain to cover only half of the
filled with
uDserve uncel tne mrcroscope. unsmmeo poBio s&lrcn grams are sneil-snapeo wlm uun
concentric lines. Iodine changes in color from golden brown to violet, blue, then blueblack in the presence of starch. Fully stained potato starch grains are blue-black in color.
BEsv
yiu tT&
##
oF
" ONLY
&r''\
kI
@uUisn - PREscsrcP oF
srARc+r
- VAq.(ENP t gLl,ck)
Starch test for Variesated
|\)o
leaf
PaR<EN\NG
u
&gScENc-E 6F
. 9rlgg
(LPC' '
EN
ce of starch?
2.
of
PARKfN't^jF MErilr
3"
4"
What color change, if any, did you observe in the grsen portions of the isaf, lasxina aiifi: addition/ of iodopotassium iodide solution? What does this indicate? t/-. THl9 i^lpiC+i-t=S iNG LEAI- TO ftur0sT ecvl OF role of the green pigment in photosyn*resis? n\s GPFqa ?\G t tNT Whal
?\\,{tL N\\\c$
tLt\t\3
AIV
,\i\nt{Es\s,
9&vTt
ruE
0t!
tS
Sftsn-z What,Knd of plant organ is the potato fuber? Can the pgtato tuber manufacture its food by photosSrnthesis?
Why? tro.
tT- lS LL TA
B.E
pomto tuber.
"TU
Y,
What can you conclude f,rom this exercise? 'T\}E ?SECF-NCE OF sTA ecU
rooD
8.* S1[-AIN \
vq
49
Exsr*is* ?4 Liberation of Oxrygeu'in Fhotosynthesis water' A part of the The free oxygen prod.r".d i-o photosynthesis comes e-ntirely from although a' large oxygen that is produced in photosynthesis rnay be used in respiration, aarouat of it is liberated into the envitonment' match' digman MATERIALS: beaker (500 ml or bigger), funnel, test tube, water, splinter'
shoots.
PROCEDUR.E: funnel at the bottorn of a As shown in Figure, place the leaff shoots of digman inside a test tube with water and inve* it 500-m1 beaker about % fiilof water. completeiy fril a be covered with a piece of over the stem of the funnel. (The mo'tl of tne test tube may ,p*f*tou piastic while being invmted i.nto the beaker with water:) See to it that there is no stari or the exercise' fube air space in the test tube at the start of rne exerurss' , i rrr_: until '" ' 2. Expose the setup to iight. observe the bubbles coming from the shoot' wait completely displaces the water in the inverted test tube' tift the test tube carefirlly and immediately insert a owing splinter into the test tube' Observe the giowing sPlinter' OBSERVATTON A.ND DISCUSStrON: lo/ Dravs a:rd label the setup in this V
i.
i.
,i
-t'--'-'-*
'l i
5;f\c
tp
tt
1
Setup to demonstrate the liberation of ory Answer the fotlowing questions: Why was the setuP exPosed tc 1i.ght?
?ROD
ng photosynthesis
)o : 9e 2_aueuA-Eldidtu
'aes,s wry"
ty' erouuwS
is?
ry
ng:s^ 6PoW Jo
0
DGqs'
.,
ueniT
TosYlu
.
Lra\rT
r,s
l-l,tNPf
/'116t
+UP Cz+s
fuoCro,
3.
,ty,
t^ll:nJ.t"pp-**d t" ihe glow.ing splinter when inserted into indicare? rur SALrweB tGN\-TTe \N.J}E* trsr r
WhV is photosynthesis imp+rtant to meur? l\Es\ tg lM ? THL C
rtON \e,
\ oF,
9$o\
THE s \q
rube? What do*s this
J.
rS
'llJE
M)PT
a{vq r!eA-Llq-^t