Estimation Dissolved Oxygen

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 Estimation Dissolved Oxygen

The Winkler’s Method

• The most famous method
• usually most accurate for relatively pure waters,
prone to errors due to interference from things
like oxidizing and reducing agents

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Estimation Dissolved Oxygen
Principle
2 MnSO4 + 4 KOH 2Mn(OH)2 + 2 K2SO4
Manganous Sulphate Manganous Hydroxide

2Mn(OH)2 + O2 2MnO(OH)2
Manganous Hydroxide Basic Manganic oxide

2MnO(OH)2 + 2H2SO4 2 MnSO4 + 4H2O + 2(O)

Manganic Hydroxide Nascent Oxygen

4KI + 2H2SO4 + 2(O) 2 K2SO4 + 2H2O+ 2 I2

4 KOH + 4KI + 4 H2SO4 + O2 4 K2SO4 + 2 I2 + 6 H2O

2 I2 + Starch Starchiodide (blue)

Starchiodide (blue) + Na2S2O3 Na2S4O6 + 2 NaI + starch

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Estimation Dissolved Oxygen
Calculations:
Concentration of hypo solution = N1
Volume of hypo solution =burette reading = V1
Volume of water sample =20 mL
Equivalent weight of oxygen =8

Volume of hypo solution of hypo solution X

ppm
Volume of water sample

1X ppm
Volume of water sample

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