project

Just looking at the project some initial things:

Taking pictures of hne slides for 12 month old with mutation and with controls
How to quantify the eyes every eye is a n 10-12 eyes in total
Pictures of themin field of view that is decided on 10x might be too big, 40x may be enough to cover the
area that is disorganized quantify the retinal layers
Count
Big disorganization neurons are all over the place
Take a bunch of pictures with those

Early crosses:
Have about 30 mice ages are close to 3 months look at gfap and nls those can be done
3 things:
When is gfap turned on within astrocycte development part
When do eyes develop
Astrocycte development

3 timelines and points to look at on the embryonic changes

Do the embryonic stuff pretty quickly within a month or so
When change look at expression of tdp
Tightness of whole story
Behavior changes worked 4 months before we chaned anything as well.
Eye is very organized when looking at histology of it
See when development happens and if theres any defect in eye development
Only difference in eyes that did and didn’t gen was turned off and others gfap was on the whole time.
Embryonic 9
To see if there are early changes between those three things because astrocytes differentiate
Driving with gfap should be in astrocytes but its not bunch of microgliosisgetting developmental art down
Jian Lee looks at developmental changes with smp mice might be able to use some e-phys
Cut sections and talk to them
Biochem and histology
Count the eyes to get the quantification from 12 month mice take pictures
- Theres any obvious effect there was an eye not showing blindness
Gfap odel is frozen embryonic model from Jackson
Histology with rat brains
Expect results
Potential pitfalls
Alternative methods / approach
2x feedback
Have rationale within aims bolded
How approach it thru databases and then outcome overarching hypothesis not dependent upon each other