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LOaut PaDsi uolay ce umde .


hoSIs dunLtic0o opo SI&
ADoptosis a foxm ppaumtd t
clen Ox
cellylu Suci cle J
ollffacmt tronm mecw s i s Pm L e i d n
cCu die clueto mjurj
Apopiusis Cm oNe Cllwu n 9 a e
deulop me elimimutefhe
CAmeNOU)_umd Uis mttcieol cels
CAmd muintim bulunie Py the 6c

um Ou Hau Hae Lelly_IoetueeM


ou cltwelnpimg-
o die cLga AMile y0uwene staL
S0 wld lnuve webble hnd.
The_ C bEtWe em ycremahnyoni fng
diee) m u p2D(PASLulle al upo otoc is
Acoonm-am poumta Ce
dluHa CellA-m clY LellulaxadLlcd
Ohem hey Pute cem tun ues
Apophosis bamitiaiu foy hol
C umis m SUdh _u eNlo p mend_
APoPO SS

DNA
ATT

chf-bemt LIMed much moeOrdedy


Shnkclnd eleulape h e
LDCCSS:InC
hublesike blcb
Smull fA mtm+ _eiMed bCylc do wn.
he_ OnuSVMlike emcdopl.us m i C
LHCUlum uHh he uSe
ofeuse Kill Hhe
YMcet i l d 0 Cely md MuCeLue
dowY the udeLy sO
DAeU
l cell une domajt
Lhy Ce ap upo psis
Mumy humaM celu huNe ubilrt o
CeLyumdc2 9 upottosik Apop-to sls_
Le Haluc hae Shguld mo lmg
PLL Dgumis m:
Some CelLL_deluttc meeel dAMi4
olAelap meu
Sone Cel_Ue CLbnc i_elnd cou
a u t Of YIM SOYme LLA
q e m fcctcd o PA demug34
ApopHosis l the (enmtehous
Cc mfe.cteel
cels
CelL ill fsef so mfertN
OrCamcexn S CEL YMO4 sDtlol
he tthe Cell
nst
3
DAT

FUnttten paptosI
Apoptosscals plAyhe imp: zo]e
he deulop memt Cmd
heulthYimmm e SHSte m Wnem
B Cmc T cell wne fiKtp20 duLe
TheN ettstecd
ADOP osis_pluy mp le im louin
mumesSteMto tP
Pnogen iLhem putthoge
OmE puthogem_cleuneel faom tae bod
luge mumh Ofells Ue m0 lmge
Aedel-emd mus he emnoNed
Apeptasis mcui tein hulamte
HAe mmume yssem
dhc budezitul-msclucto
uie.tpplittuhioM
Atleritu 1atms ductieMJ
tupeO
Jeme m s - f t hem buttoni
Ps pVA
mnsfL
AnethU_btttiu usImg
Uimis US qveLto/u
BAcHOnlo phag
DO07 PNA
LLipid pyA
Tomsfen batniu eMt by g Phg
olscoyeeel byzmclen 2 tecleAher9
M115 Salmon eil oymunum
e m i ze d
TH TaymS du ciovL
Tnsdy ch
yspeciuized
Jhe hutttniuphuge. Lntunthe
Buctal pAA Dom.Cn cm msfa
KEpi _eeCipjet_cut umcclucgphust
Bacteniophae v i s Haut inftcE
Umd epli ccLte 1Y bucR cAL
CupsiC heue

Bucter 0 p h g
PNA
Lol
-Dst Plu
tuil en . ViieE
fiber
ast

VncilumA phuge
LOiclh
follow t i C
Mftot bctcni c md i
bu cHei c mel Self
Ty Phug
Temp mutphhtje Does o t c mfct M
mse thevn
Auplicuttd 15 fotlo o CIso miC (cL
p t oj aumclutio
) 9M tmulize ctoums.cluctt
SpeciL2ed +Ms out
Aasuer lmtunA Cm U2

*AppliLctidM
CO mOn IyYStd im qemeH
eminTm Yg ee
jnezimg bstm DuA TO h0st

bucdeud mseducthlen gemerlly msuse


Cemeic dNUNSiy P4
PhLALSS C eNoluho
SezNC u Ke t AutibidHE
esistu

-10 amd mcstq sumt HI


ADplicuh ecd lud 4 uMsdutiO
Ydetail gerelingememelitcelCIne
Ctiaizcotmsduc m.

gemtrtlizeel nmsductiarn

phuge infeH + h t
Do bIcHenice col

phug
DVA

phuse ppn umd poeHeln wre


mude umd btEnl u
hxokem d o w N
Chmo SOMmE i's
mhe Pite
-Bucenjuu
BDNA
the Phuge
cCusimauby olwzin9
phige
DNA
AssmbY Pieute o bactu
DID ne packed m phu4 c s d.
Tm pomr cell 14sis Cmd euse-
Phyg pptcle cmtjmimg bycteu DNA
BHStoc
A phgae (mn baeal ell
DUA mf cth the mtu host
cel the etpitt cel
pe

RrombimdionCm occu
o ducin ombimumt
Gm botth dmoy cmd eespiet
Rerombiyufion
mHas umsdu cti oM fixs
butuiophy
Imlecd Hae domy ceu. cmdb timsy11 (1l
LwhemUms emte me ( l vius hijuck H
ost Cell uy d
sum-Hhesi2td vIYS Compomumt
Such mt Cup1e tul htud
emViul emzy me hucolYSs Vo st
LeL m o_ ShmyI DNA frigmmt

C'n o n l uia CAUu

Specicli2ed tamsduction1
P3pecli.zr d umsdu chcmbucttno plhuge
faumsfr omlfeoDNA g m m foM
clom cleni to Ae ecip eat buctnj dA.
Spe ctnliz e stuns cluchoN S ClULi te

eloaate
whem uclo ysogemi (UL y esposed
CeztiM chemicul _r UU liuK H
Cuuse 1Mduttim V i s Jtmam fwn
host Ll gomon nod mY ltic CYIe

phuge PNA ITHign it


h ga tChoe msm
detaut cmc pict tep
Piecice oburseat
Chomosom

Repttcactot Uts
DUA hd bu CGEL UDPA
ViS cugid smhesiS

NSS j b4 cn ceu Phuse eltey e


Cm d Mft cted ntw bucen celu

Umd SHubre em
cOSSoNeR
msfn in to
( wmoSome
gERI PIU
MIBrhuge inicceliuo4ph.Luti
Suticio phuge BACiCJLi Ctulers
SlplestutL
DNA RNA PeCsemt
ACp4 ltn qs tule fi DNA
Ty (5-255)
phu
MI3
P
Lymdy

M3 phug
S:S s t l uar DNA p eeut, 6yDb br lane
Rep litutid
pill S

S u n c t t e hy

M3 pnJe M3 emier Hau


Fpitls
Fpillus no PsejeLct
m Buc-enttu M13 phuse
Not ecte

Surbemd

RF Replicuhve onm
fast

2-mANA PUA+0

Photei

unuanotny_Help
9e
(
Relcse MB ALL Aeplicett

ph3

>OvA emdomucletAe

-DMA JLeplicect

13emot SS bindin sote


6:4 h
Cupsrd o t y n

fechex

+Shemcdi ctuch mepilus eteo the Lnost


leu
gene V111)
mimo
S1uey opavra0-HJ
Lovrde Jaeclieu tumsCmftiM
touSKafPh temim
imduton
USne he eno o geme V
C) 1 V
en lo gemey peotMed im Sghmeikecti o
rhu tmsfex mulle bialuiuay (rpctas

upt*e PA(ether plus 02


DA fu me):fom Hhe mtelum
by a (el emd nuin-teins P4
C heutuble -fcr
Ncttb msfr muheL
DNA_COmeA om_donr buetenjy

i n vi Uifici mir iom


PHA_(ome fo meclium m uuh

Compe-emiCeL celL thie i uble to

umserymlel_
ums fenufm f eeuantyDf d g0d c
tUwpituudfeapeled tll- 1o ohem eyess
pA ueel
emt+rsfer us methodOcg_
N_soilCnd cuguufiVE_£tosysie
mechomi sm cTeums feamutin hus
htenmie siNley gud
S. Pne ymmiq
DNA gmethinol to
Cesuafae xecesto
An cyeeLl4l1 Cmddn
Cul me puA 1Y to

One sunmd iy depuded snl a


Single smd y m spo 1 e

PNA Smd iug1 H Sel with


hovnelogo ugton on he
Racteicd cho mesm

DN stnd y ma ponj eel iuuto the

Homelegos clorbiuetra
2uM sfmec

AiCuAHscicd lempekut (eu Cuh e


muele by elechmc shocc o
CCl2 metod,
Normu hatnil

piusmicum spoo1Ec
Ht sho ck
Toumstemmee cl
3Mismuth epui CAn sosStpu
Mismudth puu
useem2Yme cine pautiL
DNA Po
DVA ligaL
Mut L Mud. SMut
SHpsl Mut s
Reco gmi2 Hhe msm utth sit
Cno bino
Mut LBimd with Mut 5

Mut H y ' dent tbetwemimeY


SmthesIs Sumd qoen &M d
u t H _cleuNe mHhe m mmtludtd

mthyluted st

eomutltcy 5 aemone nismuduM Smn_d

DNA Pol ydd mucleo He

digust seLthe up
fe oclen Conolit1 o

ActiNtd m DNA damcg


ohen DNA Modamcu 4e Ruc AJ
inctlN
whem DA domuge Rae A fottY-
uGN CLd Mhib Ley A poti
h luvim
w s Lex A motbincd te peomoten There
imdue
RNAPO LA

lwhe

whem DUA domug


Rc A teveMe

so RNA PO
Lu
s O S e A LR P A

R A AP o l
favt

hd plusmiel tpoPltsmial
E x clnemosomul,smull ulur
Supci toil tc Doublesttmlcol NA
mne rc
kclCmA m mostly cllspeicil
propeety
Cy chedmo Sommu DIUA
Replicccte mdepemdemtly
Cmttim gemesthat e non esemsicu
Ahu

usmic cCu 2 menomis


Reconbinutm pluls.mlelh Cimecommbiim
w n cheum.os0me Stntn pls mic
bhronm osmeshut amiomse1
TrumsposHia1 plusmi'e cum msenA itself|
hto Cerd m osome bY daumsposom
s.

pe o plusm'd
Conagutive 9nm Caniugztye
CPotmte 0 geme) bieme_q Tru gnd
Cm heix
6
fnt intu papperny hene C
muimcltuss
U) F lusmid
2 Cfexailty)
(ole plucmic
) DegauoletiYe
(S Vylem
) CypHC 1
u) E lusmicd
Aso Cmtum
num o other gemeseq -s plkte
R plusmio cuy tb asidumce gemes nn|
0ne ucenic 4o Anone Dapud Dat+LcH
c13ilst humuM meel) cim und b.
AnegeSi2e
( ) CoL_ plusmic
cAls oKmo un CS
Colitems avtim spmtheai1t
buGuocn0g¢mlC.
pltus m o_Hhey mcluce bcteniacm S:-
Tus_p20 jeis k)ed Closl 2luta pycBeri u
Deg uciM gaip o iusmie u c h
Jis pusmiopanicle gemeHaüt ullo uo

Vulous Ymusu metolrtes


5) pul mce Ce 4eme V oU
SYDHHaent Mpe osCLOmbiAutim

Deftmuti when-two molteajles PMA


exclhumq picue h i

Eombimudim

Homoj20u) SE0mbimuiel.
mon- Humole A
SAA Speo phC
Paplicie
fA exclhumege
gemeh mudenicu_bttweem mum-
s i s t e clomutids C HOmaldg -

n sis
C y d u h o

SISeX
ChoUmDches

Homojo q selombimior
The p0cEK e
ven
cullt css
6

OSsino ONC olui vg plhaseT


O meosis

AP NOYM Homolog6u
EAnuqe tMetie mutericubluw
Aom hondeloy cDimUsam_ueM
toatnen Quniu
terphuse tupeo tel liuisicm
Le_speLÀphic shot

seq PpA mseA_d


sit wh u emmes
Replttti ve l P occLuas by H
ACtiV _ xeplicetiY
UYSpUSOMS
(Ccnjjatiom by (olE FcastoL
SistevexC ol by Ledacr bezA Tutm
uctCzi cu to cmothc by Ccvijuaat
tubeS knaw).cs Cnugutfem
Pltismld jemeti mutei
SCo genm plonlf0

piusmic

SHep 02 FFsnephez
gemophkHO)

RepliccH
0s plusmid \
njugn tube

FT) (ACLpteN_ (elw

BucHniu c h onsistplusmld ELe


do mot huveplusmd9 F_ cell
Ruceni uhieh ConSi t bucHaju cell
melen o lpli cudi e m_(py qh
hui copy seMe s F cel Hha
Conyugadim tulb
Cmugutem tuhe Cuise otuetpreAnt

tub A cHcucla cLith laut enitl cel.

Sicp
9mohenl
phez
O F o s ptuSnate
DspTus mid

AAHCR2eplicNON_B0_ce beLome mu R
CIlal fm Ds plusnte
He Corpl ucd penimut £oli

LGuch contins plusmid F


Me talltel F done Cel

(Seplli uhseut)
F (Se pillk Dseseat)
fast

9 Lbthd is ptasmld Eplu)k plusmid


plusm d pusmidwn ea chtonasom
D NA h
CeSmull tMlur CAne
hut
bulity to_p3u due utoso an usly
They tye mosaHY fermd M
Plusmlc &e Usteo prtpyeRecormbi mmt
DMA wi+holCsrsul geme Ms fULe

gemeheneeA
T plusmic
ASD ollei amo moluig plumd
Agro bucteriumhmifuiems
size_ 200> h
MW :2x10 Y D a
t youcducegaDun Apld disEUS fmHhe
Aaau buHen ym

Vys-no Fes umst mi


TPMA CHHucn +o host DN A Z form
Pe combinumd pNA

pNA
T P l u s m e

Let RiYut
borele

boroeR

o p i n e

CCuho lis
Viluce

pltcuhon CoRi)
plusmicl
TDouble Sumd
Clstlu
Caniuquhe upt
OpineSHMHhtss

AgDuLHtajym tubifieme hus h e cibil


aumsfrm +hemmul (e O
Mmansicellhy Msti ny DUA p i U L
Of DNA P stu mclun ohemicus

AHOn nseh19 clesatel gemeIn_


1oses bilty ofpuhogemic b u t

Plunt

TDA fo plLmt (e cmd


pllt emorae o

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