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Enterococcus faecium 129 BIO 3B is classified as

Enterococcus lactis 129 BIO 3B Request full-text PDF

January 2023 · Bioscience of Microbiota Food and Health To read the full-text of this
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DOI:10.12938/bmfh.2022-088
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Authors:

Kiyofumi OHKUSU

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References (22)

Abstract

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Enterococcus faecium 129 BIO 3B is a lactic acid bacterium that has been safely used as a
probiotic product for over 100 years. Recently, concerns about its safety have arisen because
research
some species of E. faecium belong to the vancomycin-resistant enterococci. The groups of E.
20+ million members
faecium with less pathogenic potential have been split into a separate species (Enterococcus
lactis). In this study, I investigated the phylogenetic classification and safety of E. faecium 129 135+ million publication pages
BIO 3B as well as E. faecium 129 BIO 3B-R, which is naturally resistant to ampicillin. Mass
spectrometry and basic local alignment search tool analysis using specific gene regions failed
2.3+ billion citations
to differentiate 3B and 3B-R into E. faecium or E. lactis. However, multilocus sequence typing
successfully identified 3B and 3B-R as the same sequence types as E. lactis. Overall genome
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relatedness indices showed that 3B and 3B-R have high degrees of homology with E. lactis.
Gene amplification was confirmed for 3B and 3B-R with E. lactis species-specific primers. The
minimum inhibitory concentration of ampicillin was confirmed to be 2 µg/mL for 3B, which is
within the safety standard for E. faecium set by the European Food Safety Authority. Based on
the above results, E. faecium 129 BIO 3B and E. faecium 129 BIO 3B-R were classified as E.
lactis. The absence of pathogenic genes except for fms21 in this study demonstrates that
these bacteria are safe for use as probiotics.

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Citations (0) References (22)

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