ASSIGNMENT 1

BT3040

Fathima Bensha M
BS20B015

 1. Since I’m a Mac user, I have used the online tool given in the link
(https://www.bioinformatics.nl/cgi-bin/emboss).
2. The complementary strand for the given DNA sequence can be found
by the following steps:

• Open EMBOSS via the link.

• Search for the revseq function on the left-hand side of the window and
click on it.

• Copy and paste the DNA sequence into the space where the DNA
sequence can be entered manually.

 4. (i) To ind the protein sequence, use the function transeq in EMBOSS.

(ii) From the above image, it is clear that the reading frame equivalent to
the given protein sequence is 4th one (the irst reverse reading frame).

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 7. Backtranseq function

• It can be used to get the DNA sequence from a given protein

sequence. It gives the nucleic acid sequence it is most likely to have come
from.

• For each amino acid in the input sequence, the corresponding most
frequently occurring codon is used in the output nucleic acid sequence.

Geecee function

• Geecee gives the fraction of G+C bases of the entered nucleic acid
sequence(s).

• It basically sums the number of G and C bases in the sequence(s) and

writes the result as the fraction in the interval 0.0 to 1.0.

8.
(i)

 (ii)

 We can see that the melting temperature of the irst sequence with only
A and T is 48 degrees and the melting temperature of the second sequence
is 108 degrees. This indicates the as the GC content of the sequence
increases, the melting temperature as well as the stability also increases.

9.
The AT_content of the given sequence is 58.333333% and GC_content is
41.666667%.