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  • Group 8 Flowchart Methodology

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    Alexandra Tajan
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    The document describes the methodology used to test the xanthine oxidase inhibitory activity of ethanol extracts of Mimosa pudica leaves. The methodology involved collecting and authenticating M. pudica plants, preparing plant extracts by macerating leaves in ethanol and using a rotary evaporator, performing phytochemical screening tests for flavonoids and phenols, preparing plant extract concentrations from 0.5-100 ppm, preparing a positive control of allopurinol, and conducting an in vitro xanthine oxidase inhibition assay by incubating extracts and control with the enzyme and measuring inhibition.

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    GROUP-8-FLOWCHART-METHODOLOGY

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    The document describes the methodology used to test the xanthine oxidase inhibitory activity of ethanol extracts of Mimosa pudica leaves. The methodology involved collecting and authenticating M. pudica plants, preparing plant extracts by macerating leaves in ethanol and using a rotary evaporator, performing phytochemical screening tests for flavonoids and phenols, preparing plant extract concentrations from 0.5-100 ppm, preparing a positive control of allopurinol, and conducting an in vitro xanthine oxidase inhibition assay by incubating extracts and control with the enzyme and measuring inhibition.

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    8 views1 page

    Group 8 Flowchart Methodology

    Uploaded by

    Alexandra Tajan
    The document describes the methodology used to test the xanthine oxidase inhibitory activity of ethanol extracts of Mimosa pudica leaves. The methodology involved collecting and authenticating M. pudica plants, preparing plant extracts by macerating leaves in ethanol and using a rotary evaporator, performing phytochemical screening tests for flavonoids and phenols, preparing plant extract concentrations from 0.5-100 ppm, preparing a positive control of allopurinol, and conducting an in vitro xanthine oxidase inhibition assay by incubating extracts and control with the enzyme and measuring inhibition.

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    GROUP 8 Signar, Tajan, Tan and Valencia

    XANTHINE OXIDASE INHIBITORY ACTIVITY OF THE ETHANOLIC EXTRACTS OF


    MAKAHIYA (Mimosa pudica) LEAVES
    METHODOLOGY

    Plant Collection and Authentication

    Preparation of Plant Material


    Remove soil particles, air dry, grind into powder, sieve

    Preparation of Plant Extract


    Macerate in ethanol 80% and use rotary evaporator

    Phytochemical Screening

    Test for Flavonoids Test for Phenols


    • Total flavonoids • Total phenolic content
    contents (TFC) (TPC)
    • Quercetin: standard • Gallic acid: standard
    • Aluminum chloride • Folin–Ciocalteu
    colorimetric assay colorimetric method

    Preparation of Plant Extract in Various Concentrations

    Concentrations (0.5 ppm, 25 ppm, 50 ppm, 75 ppm, and 100 ppm ug/mL) will be added to
    phosphate buffer and XO enzyme → incubate → stopped by 1N hydrochloric acid

    Preparation of Positive Control


    Allopurinol with phosphate buffer

    In Vitro Xanthine Oxidase Inhibition Assay

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