Table S2. Genotypic and phenotypic characterization of M.

tuberculosis H37Rv pyrazinamide

resistant transposon mutants

Agar Medium Liquid Medium

Strain
(transposon Relevant features
POA MICa POA MIC90b PZA MIC90 INH MIC90
insertion site)
(µg ml-1) (µg ml-1) (µg ml-1) (µg ml-1)

H37Rv Parental strain 200 200 50 0.0625

pckA::Tn Disruption of gene for

3200 400-800 100 ≤0.0625
(252157) PEP carboxykinase
Disruption of gene for
panD::Tn
L-aspartate alpha- 800 >1600 100 ≤0.0625
(4043886)
decarboxylase
gabP::Tn Disruption of gene for
800 800 100 ≤0.0625
(612955) GABA permease

Rv2705c::Tn Disruption of gene for

400 400-8001 1001 ≤0.06251
(3020165) hypothetical protein

Rv2706c::Tn.1c Disruption of gene for

200 400 100 ≤0.0625
(3020304) hypothetical protein

Rv2706c::Tn.2c Disruption of gene for

400 8001 1001 ≤0.06251
(3020355) hypothetical protein

Rv3256c::Tn Disruption of gene for

400 8001 50-1001 ≤0.06251
(3636565) hypothetical protein

PclpC1::Tn Insertion in promoter

NDd 400-800 50 ≤0.0625
(4040795) region of clpC1

Rv3727::Tn Disruption of gene for

800 1600 100 ≤0.0625
(4174408) hypothetical protein
Insertion in promoter
PRv3916c::Tn
region of gene for 400 8001 1001 ≤0.06251
(4405250)
hypothetical protein
a
MIC, defined as minimum amount of drug to inhibit visible growth within 14 days on 7H10 agar
pH 6.6; bMIC90, defined as minimum amount of drug to inhibit 90% of growth relative to no drug
control in 7H9 at pH 6.6 for POA and INH, and pH 5.8 for PZA; cdenotes distinct mutants isolated
with transposon insertions in different TA sites within the Rv2706c locus; ddenotes not
determined.

1
Reported in Modlin SJ, Elghraoui A, Gunasekaran D, Zlotnicki AM, Dillon N, Dhillon N, Kuo N,
Robinhold C, Chan CK, Baughn AD, Valafar F (2021) Structure-Aware Mycobacterium
tuberculosis Functional Annotation Uncloaks Resistance, Metabolic, and Virulence Genes.
mSystems 6:e0067321.