BM

S5
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Biorisk 2B1 TEAM : 1
Management

Case Study 7 : Laboratory Inspection

LEADER :

Member :

Date : 2 JUNE 2022

 BMS533 PRAC# 7 : Laboratory Inspection

Objective

To conduct a self-inspection of a BSL-1 Laboratory

Outcomes

I. Know - the requirements for a BSL-1 lab

II. Know - how to improve the safety in a BSL-1 lab
III. Do - self-inspect a BSL-1 lab
IV. Feel - confident in using a checklist for self -inspection

Exercise

1. Using the checklist attached, conduct a self -inspection of the laboratory that you use
for this course.
2. Based on the outcomes, complete the report.
Laboratory Inspection Checklist : BSL1
Faculty of Applied Sciences, UiTM. Version 2017/1

Laboratory : BIORISK MANAGEMENT Inspector:

Premise: UiTM Kuala Pilah (Beting)
1. NUR EMILY WADINI SAIFUL AZMI
2. NURBATRISYIA HUDA KAMARUDDIN
3. NURUL ALLISYA NAJWA ABD NASIR
4. NOR SYAFIQAH ABDUL WAHID
Date: 2/6/2022

Principal Investigator: TS FAIKAH AWANG @ ISMAIL Time: 8:00 am

Check (✔ ) in the appropriate box that best describes the laboratory

A. Laboratory Facilities Yes No NA Details/Comment

1 Is a universal biohazard symbol for BSL 2 posted at the / Infectious agent is not
entrance of the laboratory? present
2 Do the sign include name, and phone number of the / Laboratory supervisor
laboratory supervisor or other responsible personnel? is responsible in
controlling the access
to laboratory
3 Is there a sink for hand washing in the laboratory? / Sink is vital for
washing hands and
apparatus
4 Is furniture in the laboratory capable of supporting /
anticipated loads and uses?
5 Are spaces between benches, cabinets, and equipment / The arrangement in
accessible for cleaning? the laboratory should
not be packed as it is
hazardous
6 Are bench tops impervious to water and resistant to /
heat, organic solvents, acids, alkalis, and other
chemicals?
7 Is furniture including bench tops covered with a non- /
porous material that can be easily cleaned?
8 Are laboratory windows that are open to the exterior /
fitted with screens?
9 Is an autoclave for pre-treatment of laboratory wastes /
is available?
10 Is an eyewash fountain available in the laboratory? / Eyewash fountain
must be located near
the exit door.
11 Is an effective integrated pest management /
programme in place and managed appropriately?
12 Is there a good housekeeping in the laboratory? / Laboratory personnel
must be well-trained.
B. Safety Equipment Yes No NA Details/Comment
13 Is there first aid kit available? /

14 Is there a fume hood for working with hazardous /

chemicals?
15 Are suitable PPE and used by laboratory personnel? / PPE is vital to prevent
contamination of
personal attire
16 Is there any storage equipment to keep biological /
materials? (e.g. refrigerator)
17 Is the equipment regularly maintained? /

C. Work Practices Yes No NA Details/Comment

18 Are there any biohazardous materials handled in the / Only involved non-
laboratory? infectious agent
19 Do personnel wash their hands before leaving the lab? /
20 Are mechanical pipetting devices used? /
21 Is there a signage available for prohibiting from eating, /
drinking, smoking, handling contact lenses, applying
cosmetics, and storing food for human consumption in
the lab?
22 Are work surfaces decontaminated with an effective / Disinfectants such as
disinfectant on completion of work especially after 70% ethanol 10%
spills or splashes of biohazardous materials? bleach are being used
to decontaminate the
spill of biohazardous
materials
23 Is a sharp bin available for disposal of and all syringes/ / It has characteristic
needles/sharps? such as thick wall and
impenetrable
24 Are re-usable sharps properly cleaned and disinfected? /
25 Is there a biological spill kit available? /
26 Are all wastes that are contaminated with bio / Generally, through the
hazardous materials autoclaved or decontaminated? use of an autoclave
27 Have all personnel been provided information about /
hazards and risk about their work activity?
28 Is laboratory waste segregated into dedicated waste / Laboratory waste
bins and labelled properly? segregated into
chemical, biological
wastes, and sharps
waste bins
29 Is there a Laboratory Biosafety Guidelines or Biosafety /
Manual in the laboratory?
 30 Is there an Emergency Response Plan? / It includes evacuation
and emergency
response information
D. Records and Documents For : Yes No NA Details/Comment
31 Procurement and transfers of biological agents /
32 Biological material /LMO inventory / It includes a
combination of facility
design, administrative
controls, and use of
Personal Protective
Equipment (PPE).
33 SOPs for contained use activity / It provides standard
operating procedures
(SOP) based on the
scope of LMO
activities
34 Staff training and competency / It provides an update
on the existing skills of
staff that enhances
their productivity
35 Equipment maintenance / It includes routine
maintenance to keep
equipment in good
working order
36 Decontamination and validation / Validation is approved
by management which
states the
decontamination
process is valid

Additional comments/observations and recommendation: (use extra paper if required)

Report

1. List two areas that will require immediate attention to improve the safety performance of this
Lab.

Area that needs immediate attention What needs to be done

1 There are no instructions or procedures for The procedure and instruction for using
using machinery apparatus such as an machinery apparatus such as an autoclave
autoclave. should be displayed on the machines.
 2 Biohazard warning signage is not present at More biohazard signage, such as corrosive,
all access points, such as autoclaves and explosive, and flammable signs, should be
laminar flow. displayed both inside and outside the
laboratory, as well as on machinery.

Example: There is no signage on the door. Signage with the necessary information was
installed.

2. If you are asked to upgrade this Lab to BSL-2, what will be the three most important areas to
upgrade?

Area to upgrade What action to take

1 Biological safety cabinet The centrifugation processes need to be

performed in a biosafety cabinet with an
aerosol-tight top, and the rotors should be
loaded and unloaded inside the cabinet.

2 Safety equipment such as sink, eyewash The station should be readily available and
station, safety shower, fire blanket, and the instructions on how to use each of the
extinguisher equipment should be displayed, so people
could use it easily during an emergency.

3 Autoclave In order to dispose of some types of

biohazardous waste in an appropriate way,
make sure to use either an autoclave or
another means of decontamination.

Example: No signage at door Attached signage with required information

Inspector’s Signature: Date: 2/6/2022

 Case study Rubric

Exceeds Mark
Attribute Meet Expectations Below Expectations
Expectations s
Marks 4-5 2-3 0 -1
1. Punctuality Submitted early Submitted on time Submitted late
Was the report submitted on
time?
2. Organization of report Well-presented and Pleasant and might Not organized
Is the prescribed format used? attractive get people to read

3. Originality of report Original, with creative Mostly written in own More than 50% of
Is the report original, or are insights words, attempts to report was
most parts duplicated from the summarise duplicated verbatim
manual?
Biological spills

4. Were the important point Yes. Points were Missed a point Only one or less
correctly identified? correctly identified. points

5. Are the recommendations for Logical and workable. Logical, but not Not very useful and
improvement logical and Will lead to entirely feasible, or little improvement.
workable? improvements not many
improvements
expected
6. Are the recommendations Full details for easy Details included, but Not enough details
sufficiently detailed? compliance still some ambiguity

Autoclave Validation

7. Form design - Is the language Clear and easy to Clear, but still a bit of Not clear.
and design clear? follow gray areas

8. Are the recommendations for Logical and workable. Logical, but not Not very useful and
improvement logical and Will lead to entirely feasible, or little improvement.
workable? improvements not many
improvements
expected
9. Are the recommendations Full details for easy Details included, but Not enough details
sufficiently detailed? compliance still some ambiguity

Laboratory Inspection Clear and easy to Clear, but still a bit of Not clear.
follow. grey areas.
10.Evaluation of the checklist.

11. Report for Improvement. Full details for easy Details included, but Not enough details
compliance still some ambiguity

12. Grammars, spellings Few, less than 3 Three to 10 mistakes More than 10
(per page) mistakes mistakes
SUM(60)

Evaluated by : Date :