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SOP of Histamine Chamber
SOP of Histamine Chamber
SOP of Histamine Chamber
1. Cleaning: Clean the outer and inner surface of histamine chamber with a clean cotton
clath carefully.
2. Operating procedure:
Fill the nibulizer with histamine.
Fit the nibulizer which is connected to monometer and the compressor to main
chamber.
Open the door and keep the animal in the histamine chamber.
Close the sliding cover.
Expose the aerosol to animal by pumping the nibulizer.
Record the time when animals (Controled and drug treated) shows preconvulsion
dyspnea.
Open the sliding cover and remove the histamine vapour.
Remove histamine from nibulizer and animal from the histamine chamber.
Clean the histamine chamber properly with a neet and clean cotton clath.
3. Precautions:
The histamine chamber must be cleaned.
Leackage of histamine vapour must be checked.
The aerosol spray must be discontinued as soon as animal develops sign of
bronchospasm, convulsions and head drop.