December 27.

2022
JINKA UNIVERSITY COLLAGE OF AGRICULTURE AND NATURAL RESOURCE
DEP.OF VETERINARY MEDICINE

Course title: Veterinary Bacteriology and Mycology

Name ______________________ ID No _______________ course code: Vetm3062

Veterinary Bacteriology and Mycology test out of 10. Time allowed: 40min

Part I: write true for correct statement and false for incorrect statement. Reason out the false
statement. (0.5point each)

1. The difference between solid and semisolid media is their agar concentration content.
2. Gelatin can be used as solidifying agent in solid media.
3. Indicator/differential media are used to differentiate between two or more types of
organisms based on their colony characteristics and colour.
4. The most common media that is used to isolate anaerobic bacteria is robertson's cooked
meat medium.
5. Culture media are a mixture of substances that promotes and supports the growth and
differentiation of microorganisms.

Part II: fill the blank spaces with correct word (0.5 each)
1. ___________ is the process of insemination of bacteria in/on suitable culture media.
2. ___________ is inoculation techniques used to inoculate bacterial broth cultures or
fluid specimens on the surface of sterile agar plates.
3. ___________ is technique of inoculation into solid media using straight wire loop.

Part III: Choose the best answer and circle only an appropriate letter (0.5point each)

1. From the following which one is not the purposes of Culturing bacteria.

A. To isolate the bacteria from specimens in pure culture

B. To study the different properties of bacteria
C. To estimate none viable bacteria cell (colony count)
D. to prepare the antigen
 December 27.2022
JINKA UNIVERSITY COLLAGE OF AGRICULTURE AND NATURAL RESOURCE
DEP.OF VETERINARY MEDICINE

2. From the following which one is make agar the selective solidifying agent than others?

A. Agar has the ability to solidify at 42 – 45 OC and liquefy at 80-100 OC

B. Agar has nutritive value for bacterial growth
C. Agars contain growth promoter substances for bacteria.
D. All

3. From the following which one is selective media that are used in bacteriology?
A. MacConkey agar B. XLD C. Mannitol salt agar D. all

4. From the following factors which one is determine the methods and procedures that we must
follow while collecting specimens for bacteriological investigation.

A. epidemiology of the disease B. clinical signs C. necropsy findings D. all

Part IV: write briefly the correct answer for the following questions on provided answer
sheet.
1. Write two growth factors that can be released from RBCs at different temperature.
(1point)

2. List the most common methods used to create anaerobiosis in laboratories (1point)

3. List three agar plate streaking techniques (1point)

 December 27.2022
JINKA UNIVERSITY COLLAGE OF AGRICULTURE AND NATURAL RESOURCE
DEP.OF VETERINARY MEDICINE

4. List the Primary identification tests used to identify bacteria to genus level. (1point)