GENE GUN

GENE GUN
CROWN GALL TUMOR
CROWN GALL TUMOR
Ti Plasmid
AGROBACTERIUM OPINES

OCTOPINE

AGROPINE

NOPALINE
OPINES
AUXIN BIOSYNTHESIS
AUXIN BIOSYNTHESIS
CYTOKININ BIOSYNTHESIS
PLANT PHENOLICS

ACETOSYRINGONE HYDROXYACETOSYRINGONE
Agrobacterium tumefaciens T-DNA
Transfer Process

➢ Bacterial colonisation
➢ Induction of bacterial virulence system
➢ Generation of T-DNA transfer complex
➢ T-DNA transfer
➢ Integration of T-DNA into plant genome
VirB proteins
 Proteins localized exocellularly forming the t-pilus

or other adhesive structures

 Mating-channel components

 Cytoplasmic membrane atpases

T DNA Transfer
CLONING PROCESS
RESTRICTION ENZYMES
RESTICTION ENZYME
Binary Vector System
Cointegrate Vector System
Cointegrate Vector System
CRY Crystal
 crystal inclusion ingestion and dissolution in the
alkaline midgut lumen
 protoxin (native protein) proteolytic activation
that turns the native Cry protein into smaller
protease-resistant toxic polypeptides;
 binding of toxin fragments to specific receptors
on the surface of midgut epithelial cells
 formation of non-selective pores permeable to
inorganic ions, amino acids and sugars