SBA # YY MM DD

1 2021 09 02
Bishakha Khatri

Title: Diffusion of substances through a membrane

Skill: ORR

Aim: To observe the process of diffusion

Hypothesis: If the starch solution changes color, then the iodine solution diffused
through the visking tubing.

Material/Apparatus:
 visking tubing
 thread
 pipette
 iodine solution
 starch solution
 beaker
 tweezers
 petri dish
 distilled water
 glass stirring rod

Procedure:
1. A piece of visking tubing was moistened and rubbed until it opened.
2. A knot was tied at one end of the tubing.
3. A pipette was used to fill the tubing with some starch solution.
4. A thread was used to tie the top of tubing tightly.
5. The tubing was rinsed with distilled water to ensure that there was no starch
solution on the outside of the tubing.
6. Iodine solution was placed into a beaker.
7. The visking tubing was gently placed into the iodine solution.
8. The apparatus was left for ten minutes.

FOR TEACHERS ONLY

SKILLS ASSESSED M/M ORR A/I P/D D CL/F
MARK OBTAINED
 SBA # YY MM DD
1 2021 09 02
Bishakha Khatri
Diagram

DIAGRAM SHOWING DIFFUSION OF IODINE SOLUTION INTO STARCH SOLUTION

THROUGH VISKING TUBING

Observations/Results:

Table showing color changes of solutions after diffusion

The color before ten minutes The color after ten minutes

The color of the starch solution white blue-black

in the tubing
The color of the iodine solution orange-brown orange-brown
in the beaker

FOR TEACHERS ONLY

SKILLS ASSESSED M/M ORR A/I P/D D CL/F
MARK OBTAINED
 SBA # YY MM DD
1 2021 09 02
Bishakha Khatri
Analysis:
Diffusion is the movement of molecules from a region of higher concentration to a
region of lower concentration. When the visking tubing filled with starch solution
was placed into the beaker with iodine solution, the starch solution was white.
After being placed in the iodine solution for ten minutes, the starch solution in the
visking tubing was a blue-black color. The starch solution changed color because
the iodine solution diffused through the visking tubing into the starch solution.
The starch solution turned blue-black in the presence of iodine solution due to
amylose, a component in starch, that is responsible for the formation of blue color
in the presence of iodine. The iodine solution was able to diffuse through to the
visking tubing due to it only allowing small molecules to pass through it whilst the
starch solution was unable to diffuse through the visking tubing because the starch
molecules were too large and too complex to pass through the membrane.

Conclusion:
In conclusion, diffusion was observed in this experiment. The iodine solution
diffused through the visking tubing causing the starch solution to turn blue-black.
The starch solution was unable to diffuse due to larger, more complex molecules.

Reflection;
Through this experiment, I learnt that not all substances are able to diffuse due to
the size of their molecules. Substances with larger, more complex molecules, like
starch, are unable to diffuse, whilst, substances with smaller molecules, like
iodine, are able to diffuse through membranes. Diffusion takes places many times
in our daily lives; when we breathe, oxygen diffuses from the air into our blood,
when we eat, calcium diffuses from the food to our cells in the small intestine.

FOR TEACHERS ONLY

SKILLS ASSESSED M/M ORR A/I P/D D CL/F
MARK OBTAINED