Gent ds) Kenya Agricultural & Livestock Research Organization Food Crops Research Centre - Kabete P.O Box 14733-00800; Tel 020-2464435, Email: cd.narl@kalro.org Ref: KALRO/KAB/SOILS/BAC/5S69/2023 Date: 3 March, 2023 SEVEN SEEDS LTD FARM. 0723465165, RE: SOIL SAMPLE The soil samples brought for pathological analysis refers. Diagnosis Analysis was carried out specifically for Ralstonia solanacearum but this bacterium was not detected. This bacterium causes bacterial wilt disease which leads to wilting and eventual death of plants. Management Strategies: Since the land from which the sample was taken appears free of the bacterium, infestation should be prevented by adopting the following precautions: 1. Disinfect farm implements (e.g. pangas, jembes, ploughs, gumboots, shoes, pegs and sprinkler stands) before use. A jik solution (dilution of 500 ml in 10 litres of water) can be used for washing the implements. This will prevent introduction of the bacterium from potentially affected fields. 2, Control run-off water which may be coming from affected areas to avoid introduction of the bacterium. You should also ensure that workers start working from the disease-free areas before proceeding to the affected parts of the field. 3. Start-off seedlings/seed should be clean [certified from reliable sources. Please do not hesitate to contact us for any further assistance and/or feedback. r. Ruth Amata Head of Plant Pathology Section, KALRO-Kabete KALRO KABETE P.O.BOX 14733-00800 Nairobi KENYA. Tel:254-0202464435 Email cd.narl kairo.or Website:www.kalro.org CAUTION: THE RESULTS ABOVE SHOULD ONLY BE USED FOR ADVISORY PURPOSES IN THE MANAGEMENT OF THE IDENTIFIED PATHOGEN.KALRO ena naneotoal venice Reseach v/ Kenya Agricultural & Livestock Research Organisation Food Crops Research Centre - Kabete P.O Box 14733-00800; Tel 020-2464435 Email:cd.narl@kalro.org Ref: KALRO/KAB/SOILS/BAC/569/2023 Date: 3 March, 2023 SEVEN SEEDS LTD FARM. 0723465165 RE: SOIL SAMPLE FOR NEMATODE ANALYSIS The soil samples brought to the Plant Pathology Section for nematode analysis refer. Results: The following nematodes were extracted from the soil sample. | Nematodes Identified | No. of nematodes in 200cc of soil ] Plant-parasitic nematodes None Free-living (non-parasitic nematodes) none No plant parasitic nematodes were found in the soil sample. Only free-living nonparasitic) nematodes which are not harmful to plants were found present Management recommendations: Although no plant parasitic nematodes were identified from the soil sample, care should be taken to avoid introduction of nematodes through infected seedlings, manure and water for irrigation. Please do not hesitate to contact us for any further assistance and/or feedback. Dr. Ruth Amata Head Plant Pathology Section/KARLO-Kabete CAUTION: THE RESULTS ABOVE SHOULD ONLY BE USED FOR ADVISORY PURPOSES IN THE MANAGEMENT OF THE IDENTIFIED PATHOGEN hasten: POL 2 P.O.BOX 14733-00800, Nairobi, KENYATel:254-0202464435 Email ed.nari@ kal(Pav &\\ KALRO (Kenya Rei! Etech ee Kenya Agricultural & Livestock Research Organisation Food Crops Research Centre - Kabete P.O Box 14733-00800; Tel 020-2464435 Email:cd.narl@kalro.org Ref: KALRO/KAB/SOILS/BAC/569/2023 Date: 3 March, 2023 SEVEN SEEDS LTD FARM. 0723465165 RE: SOIL SAMPLE The soil samples brought for pathological analysis refers. Results: ‘A fungus, Fusarium sp, was isolated (detected through laboratory analysis) in the soil samples. Strains of Fusarium may cause diseases in a wide range of crops. In particular, strains of Fusarium oxysporum may cause root rots and wilt diseases in horticultural crops including beans, tomato, capsicum, sweet pepper, cucurbits, etc. Fusarium solani may cause stem cankers in tree crops and most vegetable crops. Management Strategi In order to reduce the risk of Fusarium-caused diseases the following measures should be taken: 1. Always plant certified, disease-free seeds or materials to avoid introduction of additional pathogens (disease-causing organisms) on your farm. 2. Scout fields/crops for early disease detection and management. 3. Ensure proper drainage in your soils as waterlogged conditions favor some soil borne diseases. 4. Disinfect farm tools to prevent spreading the pathogen from one area of the farm to another. 5 Apply bio-pesticides containing Trichoderma (e.g. Rootgard, Trianum-P, Eco-T or Trichotech) either as a root-dip before planting or as a soil drench after planting. KALRO KABETE DE ron aad 76 Drench the soil with fungicides such as Previcur N before planting or during plant growth to control soil borne pathogens including Fusarium spp. Always follow the manufacturers’ recommendations. 7 Itis advisable to integrate (combine) as many control measures as possible. Please do not hesitate to contact us for any further assistance and/or feedback. Dr. Ruth Amata Head Plant Pathology Section/KALRO-Kabete. CAUTION. THE RESULTS ABOVE SHOULD ONLY BE USED FOR ADVISORY PURPOSES IN THE MANAGEMENT OF THE IDENTIFIED PATHOGEN Pehcuaig