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Microbiology Urine-Culture
Microbiology Urine-Culture
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GRAM STAIN
⮚ Wet mount results should be available 1 hour after specimen
⮚ As many as 60% to 80% of all urine specimens received for culture report
by the acute care medical center laboratory may contain no ⮚ Isolation of a possible pathogen can be expected after 2-3 days
etiological agents of infection. Procedure developed to identify ⮚ Negative culture will be reported out 1-2 days after the receipt of
quickly those urine specimens that will be negative on culture, the specimen
thus to circumvent excessive use of media, technologist time, and
Additional Information
the overnight incubation period. The gram stain is the easiest,
least expensive, and probably the most sensitive and reliable
⮚ A single culture is about 80% accurate in the female, two
screening method for identifying urine specimen that contain
containing the same organism with count of 10’5 or more
greater that 105 CFU/ml
represents 95% chance of true bacteriuria., three such specimens
▪ A drop of well-mixed in urine is allowed to air dry
mean virtual certainty of true bacteriuria.
▪ The smear Is stained and examined under of
⮚ Single clean voided specimen from an adult made may be
immersion (1000X)
considered diagnostic with proper preparation and care in
▪ Presence of at least one organism per oil immersion
specimen collection
field (examining 20 fields) correlates with significant
⮚ UTI is significantly higher in woman who use diaphragm
bacteriuria (> 105 CFU/ml)
spermicide contraception, perhaps secondary to increased vaginal
pH and a higher frequency of vaginal colonization with E.coli
URINE
⮚ Most laboratories limit the number of organisms which will be
identified when recovered from urine to two.
⮚ If the patient is receiving antimicrobial therapy at the time the
BLOOD AGAR MACCONKEY AGAR NUTRIENT AGAR
specimen is collected, any level of bacteriuria may be significant
⮚ Failure to recover aerobic organisms from patients with pyuria or
Culturing Procedure positive Gram’s stains of urinary sediment may indicate the
presence of mycobacteria or anaerobes.
⮚ Mix the urine sample to re-suspend microorganism present.