Figure-1 (Han) A O57BL/6J mice Light stimulation retrograding PRV-Cre AAV-DIO- ChR2-eYFP AAV-DIO-ChR2 — PRV-Cre (NAC) Subthveshold “Socal WTA) Femule (VTA) sess interaction t ¢ = \Dayi————— 14. ———- 17 ——— 8 d TH.cre mice bat stimulation retrOgrading AAV2I5-DIO- ChR2-eYFP A216 D10-ChR2 Subtweshold Socal (ac) Femulg (VTA) ages. interaction is is ¢ % Iay 1 _———— 4 ——— 17 Q Stress-naive mice ARV-DIO-ChR2 —_ PRV.Gre (NAC) Social (TA) Ferule (VTA) _ interaction + + ¥ Day1 ———— 14 ———— 18 J Stress-naive mice roa. Chronic AAV-DIO-ChR2 PRV.Cro(NAc) stimulation » Social (via) Ferrule (VTA) [z~=| interaction ¥ ¥ + + Day1 ——— 14——— 17021 —— 22 — 23 o ° evrp - phasic TCHR? - Tonic 8 2 Time in interaction zone (s) No target Target Time in interaction zone (s) = = ‘Time in interaction zone (s) No target Target 8 a in interaction zone (s) = Fold change (normalized to GAPDH) or GAPDH OP Fold change § (normalizedto GAPDH) Z GAPDH — — 8 5 Fold change (normalized to GAPDH) 88s a 8 \ ) ) GAPDH —— 15 1.0 as Fold change (normalized to GAPDH) 00 eONF GAPDH eeFigure-2 (Han) @ C57BL/6J mice Light stimulation retrograding PRV-Cre, AAY-DIO- ChR2-eYFP. PRV-Cre (NAc) ‘AAV.DIO-ChR2. Cannula (NAC) subthreshold ANA-12 "Social wy) Femrule (VIA) stress Hr PHOr interaction ss |Day1 ——— 14 ———-17. | 1% |——19| d MBDNF mice Light stimulation retrograding PRV-Cre AAV-DIO- ChR2-eYFP- ‘AAV-DIO-ChR2PRV-Cre(NAc} Subthreshold Social (VTA) Ferrule (VTA) stress. interaction + + + + IDay 1 14 7 8 Sac + 49] ‘Time in interaction zone (2) Time in interaction zone (2) aeVFP + ANA-12 ECHR? + Vehicle BEIChR2 + ANA-12 No target Target ENon-#n BDNF mf BDNF No target Target c Fold change (normalized to GAPDH) Fold change (normalized to GAPDH) 8 2 ¢ GAPDH ——Figure-3 (Han) ac BLGJ- st Light stimulation AAV-DIO- -ChR2-eYFP PRV-Cre (NAC) CRF ‘AAV-010-ChR2 Cannula (NAc) Subtiresheld antagonist Social ra) Ferrule(VTA) sess. tr rir interaction Sac wi te + [pay 4 1—| 8 1 d C57BU6J- stress-naive mice CRF infusion Light stimulation retrograding PRV.Cre PRV.Cre (NAc) AAUDIO-CHRE Camuta (NAC) onr (iA) Ferule (VIA) 4 hrrior interaction Sac vt - = Jay? ia —| i J C57BLI6J- stress-naive mice oRF infusion CRF Social ‘hr pir interaction Sac Feri (TA) ri sa [Day1 14 |———15] o ‘Time in interaction zone (s) Time in interaction zone (s) ‘Time in interaction zone (s) YEP + Vehicle hr? + Veniele EChR2 + CRF Antagonist 100: 75; 50 28 No target Target eVFP + Vehicle TE ChR2 + CRF ECHR? + Vehicle 1009 75 50. 25 No target Target venice Wt ug CRF $08 ug CRF 75 50 28: 0 Notarget Target Fold change (normalizes to GAPDH) 20. a 15. 10 Fold change as: Fold change (normalized te GAPDH)Supplemental Figure-1 (Han) a, A/P+1.10mm 2-4 -6 -8Supplemental Figure-2 (Han)Supplemental Figure-3 (Han) ae EeeSupplemental Figure-4 (Han) Naive & 100 7 mm Subthreshold Defeat b Fold change (normalized to GAPDH) 5 0.5: Time in interaction zone (s) 0.0. —< No target Target ee * GAPDHSupplemental Figure-5 (Han) 50 mV 200 msSupplemental Figure-7 (Han) a C57BL/6J - stress-naive mice b 100: i = Co eYFP - Vehicle z2 = 20 eYFP- CRF 2 5 I ChR2 - CRF/Tonic Qa 8 75) as 15 g S2 10 = 50 3B o SN € Po 05 2 = 5 0.0 c . E & Ee 0 NoTarget TargetSupplemental Figure-6 (Han) @ C57BL/6J - stressed mice b 100 2.07 CeYFP- Vehicle @@ eYFP - CRF Antagonist 75: 50: Fold change (normalized to GAPDH) 25: Time in interaction zone (s) 0. No Target TargetSupplemental Figure-8 (Han) b 33: BDNF °° CRF «» Dopamine TrkB CRF Dopamine RD pag 2 °0° oe receptor receptor receptor iring \ / > : ae Gating BDNF up-regulation NAc VTA Medium Spiny Neuron Dopamine Neuron